Delivery of antisense oligonucleotides using multi-layer coated gold nanoparticles to methicillin-resistant S. aureus for combinatorial treatment

Beha, Marcel Janis; Ryu, Jea Sung; Kim, Yang Soo; Chung, Hyun Jung

doi:10.1016/j.msec.2021.112167

Cited by 24 publications

(25 citation statements)

References 48 publications

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“…The target DNA hybridized with the probes binds with anti-FAM antibody coated on gold nanoparticles (AuNP-aFAM), resulting in a colorimetric 'red signal' on the test line. We demonstrate that the current RCA-LFA method can be used to detect mecA, which is known as the antibiotic resistance gene for methicillin-resistant Staphylococcus aureus (MRSA) [34]. We anticipate that the current assay, with advantages in enabling amplification of nucleic Fig.…”

Section: Introductionmentioning

confidence: 90%

A Lateral Flow Assay for Nucleic Acid Detection Based on Rolling Circle Amplification Using Capture Ligand-Modified Oligonucleotides

et al. 2022

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We introduce a lateral flow assay (LFA) integrated with a modified isothermal nucleic acid amplification procedure for rapid and simple genetic testing. Padlock probes specific for the target DNA were designed for ligation, followed by rolling circle amplification (RCA) using capture ligand-modified oligonucleotides as primers. After hybridization with detection linker probes, the amplified target DNA is flowed through an LFA membrane strip for binding of gold nanoparticles as the substrate for colorimetric detection. We established and validated the “RCA-LFA” method for detection of mecA, the antibiotic resistance gene for methicillin-resistant Staphylococcus aureus (MRSA). The assay was optimized using various concentrations of primers and probes for RCA and LFA, respectively. The sensitivity was determined by performing RCA-LFA using various amounts of mecA target DNA, showing a detection limit of ~ 1.3 fmol. The specificity of the assay was examined using target DNAs for other resistance genes as the controls, which demonstrated positive detection signals only for mecA DNA, when added either individually or in combinations with the control targets. Furthermore, applying the RCA-LFA method using specifically designed probes for RNA-dependent RNA polymerase (RdRp) and receptor binding domain (RBD) gene for SARS-CoV-2, which demonstrated feasibility of the method for viral gene targets. The current method suggests a useful platform which can be universally applied for various nucleic acid targets, allowing rapid and sensitive diagnosis at point-of-care. Supplementary Information The online version contains supplementary material available at 10.1007/s13206-022-00080-1.

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Section: Introductionmentioning

confidence: 90%

A Lateral Flow Assay for Nucleic Acid Detection Based on Rolling Circle Amplification Using Capture Ligand-Modified Oligonucleotides

et al. 2022

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“…This nanoformulation showed superior antibacterial activity against MRSA and was able to remove the biofilm within 48 h [ 286 ]. Multi-layer coated AuNPs fabricated by surface immobilization of AuNPs with polyethylenimine and loaded with antisense oligonucleotides were found to be internalized into MRSA, S. epidermidis , and Bacillus subtilis cells; MRSA caused silencing of the mecA gene in a dose-dependent manner up to 74% with high selectivity, and in the presence of a β-lactam antibiotic oxacillin bacterial growth, inhibition was ca 71%, suggesting recovery of antibacterial sensitivity [ 287 ]. Nanocargos of AuNPs conjugated to ε-polylysine and octadecanethiol (C18) inhibited carbapenem-resistant Acinetobacter baumannii and MRSA with 15–20-fold higher efficiency than free ε-polylysine (MIC ranging from 8 to 15 μg/mL) and can be applied for the effective prevention of biofilm formation in both resistant bacterial strains [ 288 ].…”

Section: Applied Nanomaterialsmentioning

confidence: 99%

Advances in Nanostructures for Antimicrobial Therapy

Jampílek

Kráľová

2022

Materials

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Microbial infections caused by a variety of drug-resistant microorganisms are more common, but there are fewer and fewer approved new antimicrobial chemotherapeutics for systemic administration capable of acting against these resistant infectious pathogens. Formulation innovations of existing drugs are gaining prominence, while the application of nanotechnologies is a useful alternative for improving/increasing the effect of existing antimicrobial drugs. Nanomaterials represent one of the possible strategies to address this unfortunate situation. This review aims to summarize the most current results of nanoformulations of antibiotics and antibacterial active nanomaterials. Nanoformulations of antimicrobial peptides, synergistic combinations of antimicrobial-active agents with nitric oxide donors or combinations of small organic molecules or polymers with metals, metal oxides or metalloids are discussed as well. The mechanisms of actions of selected nanoformulations, including systems with magnetic, photothermal or photodynamic effects, are briefly described.

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“…Due to their extrinsic positive charge, ASO nanocarriers based on electrostatic adsorption are usually prone to nucleic acid leakage through the formation of polyelectrolyte aggregates and induce excessive positive charge-related cytotoxicity and non-specific interactions with serum or plasma proteins, but most of them have been used successively to deliver siRNA and mRNA with good results, however, only a few of which have been used to attempt the delivery of ASOs. Marcel developed a nanoparticle-based delivery system for ASOs targeting the antibiotic resistance of methicillin-resistant Staphylococcus aureus (MRSA): the system was prepared by the sequential modification of gold nanoparticles with PEI and maintained antibacterial ability with reduced low cytotoxicity [107]. Yoshida succeeded in solving the problem of poor intracellular uptake by target cells by using superparamagnetic iron oxide (SPIO) nanoparticles coated with PEI as a delivery vehicle for ASOs [108].…”

Section: Dendrimersmentioning

confidence: 99%

“…8A). A multi-layer coated gold nanoparticles (MLGNPs) delivering antisense oligonucleotides (ASOs) were shown to be efficiently internalized into various types of Gram-positive bacteria and may use with conventional antibiotics [107] (Fig. 8B).…”

Section: Metallic Nanoparticles Systemsmentioning

confidence: 99%

Nonviral delivery systems for antisense oligonucleotide therapeutics

Huang

Hao

et al. 2022

Biomater Res

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Antisense oligonucleotides (ASOs) are an important tool for the treatment of many genetic disorders. However, similar to other gene drugs, vectors are often required to protect them from degradation and clearance, and to accomplish their transport in vivo. Compared with viral vectors, artificial nonviral nanoparticles have a variety of design, synthesis, and formulation possibilities that can be selected to accomplish protection and delivery for specific applications, and they have served critical therapeutic purposes in animal model research and clinical applications, allowing safe and efficient gene delivery processes into the target cells. We believe that as new ASO drugs develop, the exploration for corresponding nonviral vectors is inevitable. Intensive development of nonviral vectors with improved delivery strategies based on specific targets can continue to expand the value of ASO therapeutic approaches. Here, we provide an overview of current nonviral delivery strategies, including ASOs modifications, action mechanisms, and multi-carrier methods, which aim to address the irreplaceable role of nonviral vectors in the progressive development of ASOs delivery.

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Delivery of antisense oligonucleotides using multi-layer coated gold nanoparticles to methicillin-resistant S. aureus for combinatorial treatment

Cited by 24 publications

References 48 publications

A Lateral Flow Assay for Nucleic Acid Detection Based on Rolling Circle Amplification Using Capture Ligand-Modified Oligonucleotides

A Lateral Flow Assay for Nucleic Acid Detection Based on Rolling Circle Amplification Using Capture Ligand-Modified Oligonucleotides

Advances in Nanostructures for Antimicrobial Therapy

Nonviral delivery systems for antisense oligonucleotide therapeutics

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