Delivery of functional polyelectrolytes from complexes induced by salt addition: Impact of the initial binding strength

Rondon, Céline; Argillier, Jean-François; Leal-Calderón, Fernando

doi:10.1016/j.jcis.2014.08.073

Cited by 4 publications

(2 citation statements)

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“…This is consistent with earlier reports that show that PAH/PSS PECs may even gain stability in the presence of up to 3 or 4 m NaCl. [ 41,42 ] PDADMAC/PSS PECs or multilayers have demonstrated the ability to form stable PECs in the presence of up to 2 m NaCl. [ 38,39 ] Lysozyme release from PDADMAC/PSS PECs is then more likely a result of reduced attraction between the PEC and lysozyme, which has a much lower charge density than the polyelectrolytes.…”

Section: Resultsmentioning

confidence: 99%

Extraction of Lysozyme from Chicken Albumen Using Polyelectrolyte Complexes

Lente

Lindhoud

2021

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Cells use droplet‐like membrane‐less organelles (MLOs) to compartmentalize and selectively take‐up molecules, such as proteins, from their internal environment. These membraneless organelles can be mimicked by polyelectrolyte complexes (PECs) consisting of oppositely charged polyelectrolytes. Previous research has demonstrated that protein uptake strongly depends on the PEC composition. This suggests that PECs can be used to selectively extract proteins from a multi‐protein mixture. With this in mind, the partitioning of the protein lysozyme in four PEC systems consisting of different weak and strong polyelectrolyte combinations is investigated. All systems show similar trends in lysozyme partitioning as a function of the complex composition. The release of lysozyme from complexes at their optimal lysozyme uptake composition is investigated by increasing the salt concentration to 500 mm NaCl or lowering the pH from 7 to 4. Complexes of poly(allylamine hydrochloride) and poly(acrylic acid) have the best uptake and release properties. These are used for selective extraction of lysozyme from a hen‐egg white protein matrix. The (back)‐extracted lysozyme retains its enzymatic activity, showing the capability of PECs to function as extraction media for proteins.

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Section: Resultsmentioning

confidence: 99%

Extraction of Lysozyme from Chicken Albumen Using Polyelectrolyte Complexes

Lente

Lindhoud

2021

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“…The ability to attain control over the electrostatic and hydrophobic interactions that dictate the formation of liquid crystalline phases in these systems is advantageous in manipulation of structure and rendering them responsive to external stimuli. Variables that have impacted on the phase behavior, stability, and/or size of nanostructures formed in these systems include temperature, − salt concentration, − and pH . The system consisting of the industrially relevant anionic surfactant, sodium dodecyl sulfate, and the cationic polymer, poly(diallyldimethylammonium chloride), has been shown to form hexagonal phase-structured capsules, whereas the biorelevant anionic bile salt, sodium taurodeoxycholate, and the cationic polymer, chitosan, formed lamellar mesophase within the capsule shell .…”

Section: Introductionmentioning

confidence: 99%

Controlling the Mesostructure Formation within the Shell of Novel Cubic/Hexagonal Phase Cetyltrimethylammonium Bromide–Poly(acrylamide-acrylic acid) Capsules for pH Stimulated Release

Tangso

Patel

Lindberg

et al. 2015

ACS Appl. Mater. Interfaces

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The self-assembly of ordered structures in mixtures of oppositely charged surfactant and polymer systems has been exploited in various cleaning and pharmaceutical applications and continue to attract much interest since their discovery in the late twentieth century. The ability to control the electrostatic and hydrophobic interactions that dictate the formation of liquid crystalline phases in these systems is advantageous in manipulation of structure and rendering them responsive to external stimuli. Nanostructured capsules comprised of the cationic surfactant, cetyltrimethylammonium bromide (CTAB), and the diblock copolymer poly(acrylamide-acrylic acid) (PAAm-AA) were prepared to assess their potential as pH responsive nanomaterials. Crossed-polarizing light microscopy (CPLM) and small-angle X-ray scattering (SAXS) identified coexisting Pm3n cubic and hexagonal phases at the surfactant-polymer interface. The hydrophobic and electrostatic interactions between the oppositely charged components were studied by varying temperature and solution pH, respectively, and were found to influence the liquid crystalline nanostructure formed. The lattice parameter of the mesophases and the fraction of cubic phase in the system decreased upon heating. Acidic conditions resulted in the loss of the highly ordered structures due to protonation of the carboxylic acid group, and subsequent reduction of attractive forces previously present between the oppositely charged molecules. The rate of release of the model hydrophilic drug, Rhodamine B (RhB), from nanostructured macro-sized capsules significantly increased when the pH of the solution was adjusted from pH 7 to pH 2. This allowed for immediate release of the compound of interest "on demand", opening new options for structured materials with increased functionality over typical layer-by-layer capsules.

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