Delivery of gadolinium-labeled nanoparticles to the sentinel lymph node: Comparison of the sentinel node visualization and estimations of intra-nodal gadolinium concentration by the magnetic resonance imaging

Kobayashi, Hisataka; Kawamoto, Satomi; Bernardo, Marcelino; Brechbiel, Martin W.; Knopp, Michael V.; Choyke, Peter L.

doi:10.1016/j.jconrel.2005.12.019

Cited by 142 publications

(108 citation statements)

References 51 publications

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“…Recently, new MR imaging agents consisting of polyamidoamine dendrimers have been developed for preoperative characterization of lymphatic drainage and lymph node metastases from mammary tumors (8,9). These studies demonstrated that the superior temporal and spatial resolution of micro-MR imaging facilitates the identification of lymphatic metastasis in experimental animals.…”

Section: Discussionmentioning

confidence: 99%

“…4A-4D). At 24 h time point, the fluorescence intensity of the resected lymph nodes were 7.73×10 9 and 16.2×10 9 photons/ sec at the injected dose of 0.002 mmol Gd/kg and 0.02 mmol Gd/kg, respectively (p = 0.005). On MR imaging, at the higher dose level, both axillary and brachial nodes were already identifiable at 3 min after contrast injection (Fig.…”

Section: Dual Mr-optical Imaging Detection Of Axillary and Brachial Lmentioning

confidence: 96%

“…To date, several imaging techniques, including ultrasound (4), computed tomography (5), and MR imaging (6)(7)(8)(9), have been tested to minimize some of the limitations associated with the standard approach to SLN evaluation. In particular, MR imaging has been used not only to localize SLNs but also to detect lymph node abnormalities.…”

Section: Introductionmentioning

confidence: 99%

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Development of a Macromolecular Dual-Modality MR-Optical Imaging for Sentinel Lymph Node Mapping

Melancon¹,

Wang²,

Wen³

et al. 2007

Investigative Radiology

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Objective-To evaluate the effectiveness of a dual magnetic resonance-near infrared fluorescence optical imaging agent, poly(L-glutamic acid)-DTPA-Gd-NIR813, for both preoperative and intraoperative visualization and characterization of sentinel lymph nodes (SLN) in mice.Materials and Methods-Poly(L-glutamic acid) was conjugated with DTPA-Gd and NIR813 dye to obtain PG-DTPA-Gd-NIR813. To confirm drainage into the SLNs, this agent was injected subcutaneously into the front paw of nude mice followed by isosulfan blue (n = 6). Furthermore, PG-DTPA-Gd-NIR813 was injected subcutaneously at doses of 0.002 mmol Gd/kg (4.8 nmol eq. NIR813) and 0.02 mmol Gd/kg (48 nmol eq. NIR813) (n = 3/dose). To differentiate metastatic from non-metastatic lymph nodes, nude mice bearing human oral squamous cell carcinoma (DM14) were injected intralingually with 0.02 mmol Gd/kg PG-DTPA-Gd-NIR813 (n=3). Pre-and post-contrast images were taken using 4.7T Bruker MRI scanner and Xenogen optical imaging system. The status of lymph nodes resected under the guidance of optical imaging was confirmed by histologic examinations.Results-PG-DTPA-Gd-NIR813 co-localized with isosulfan blue, indicating drainage to the SLN. After subcutaneous injection, axiliary and branchial lymph nodes were clearly visualized with both T1-weighted MR and optical imaging within 3 min of contrast injection, even at the lowest dose tested (0.002 mmol Gd/kg). After intralingual injection in tumor-bearing mice, MR imaging identified 4 of the 6 superficial cervical lymph nodes, whereas NIRF optical imaging identified all 6 cervical nodes. The pattern of contrast enhancement of SLN visualized in MR images showed a characteristic ring-shaped appearance with a central filling defect, possibly resulting from nodal infiltration of metastatic lesions. Histopathologic examination of the SLNs resected under NIRF imaging guidance revealed micrometastases in all 6 SLNs identified by NIRF imaging.

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Section: Discussionmentioning

confidence: 99%

Section: Dual Mr-optical Imaging Detection Of Axillary and Brachial Lmentioning

confidence: 96%

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Development of a Macromolecular Dual-Modality MR-Optical Imaging for Sentinel Lymph Node Mapping

Melancon¹,

Wang²,

Wen³

et al. 2007

Investigative Radiology

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“…59 As well as UPIO the gadolinium chelates have a small particle size which leads to poor retention in the lymphatic vessels and diffusion out of the vessels leads to such difficulties in interpretation as the background noise. 60 A meta-analysis of the investigation employed in different body regions has shown the sensitivity and specificity of 90% and 96%, respectively. 61 The method of MRI lymphography is regarded to be an attractive option for SLN investigation as it is non-invasive and provides anatomic and functional information but the presence of an experienced radiologist is essential.…”

Section: 54mentioning

confidence: 99%

A Review of Techniques for Sentinel Lymph Node Mapping with Special Reference to the Head and Neck Region

Sun¹

2016

ICPJL

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Sentinel lymph node biopsy is an extensively explored diagnostic mean for reducing surgical risks while improving staging in different human malignancies. Several different techniques have been introduced to clinical use during last two decades. Methods as ink lymphography and lymphoscintigraphy have already established their position in sentinel nodes mapping. Novel approaches as CT, MRI-lymphography, contrast enhanced ultrasound and near-infrared fluorescence imaging possesses prominent potential advantages and demand further experience gaining. A review of the clinically utilized techniques is presented a special attention is drawn to their usefulness in the head and neck carcinomas work-up. Keywords

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“…Gadolinium-labeled dendrimers with a coreshell structure have also been used successfully to visualize lymph node metastases. [12][13][14] Further, indocyanine green, a near-infrared fluorescence (NIRF) probe approved by the US Food and Drug Administration, has exhibited potential in SLN mapping applications for breast cancer and gastric cancer. [15][16][17][18] However, the very short half-life of indocyanine green in the blood (approximately 2-4 minutes) is its primary drawback as a molecular imaging probe.…”

mentioning

confidence: 99%

Accurate sequential detection of primary tumor and metastatic lymphatics using a temperature-induced phase transition nanoparticulate system

Oh¹,

Yhee²,

Lee³

et al. 2014

IJN

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Primary tumor and tumor-associated metastatic lymphatics have emerged as new targets for anticancer therapy, given that these are difficult to treat using traditional chemotherapy. In this study, docetaxel-loaded Pluronic nanoparticles with Flamma™ (FPR-675, fluorescence molecular imaging dye; DTX/FPR-675 Pluronic NPs) were prepared using a temperature-induced phase transition for accurate detection of metastatic lymphatics. Significant accumulation was seen at the primary tumor and in metastatic lymph nodes within a short time. Particle size, maximum drug loading capacity, and drug encapsulation efficiency of the docetaxel-loaded Pluronic NPs were approximately 10.34±4.28 nm, 3.84 wt%, and 94±2.67 wt%, respectively. Lymphatic tracking after local and systemic delivery showed that DTX/FPR-675 Pluronic NPs were more potent in tumor-bearing mice than in normal mice, and excised mouse lymphatics showed stronger near-infrared fluorescence intensity on the tumor-bearing side than on the non-tumor-bearing side at 60 minutes post-injection. In vivo cytotoxicity and efficacy data for the NPs demonstrated that the systemically administered NPs caused little tissue damage and had minimal side effects in terms of slow renal excretion and prolonged circulation in tumor-bearing mice, and rapid renal excretion in non-tumor-bearing mice using an in vivo real-time near-infrared fluorescence imaging system. These results clearly indicate that docetaxel-loaded Pluronic NPs could provide a strategy to achieve effective cancer therapy by simultaneous delivery to primary tumors, tumor lymphatics, and tumor-associated metastatic lymphatics.

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Delivery of gadolinium-labeled nanoparticles to the sentinel lymph node: Comparison of the sentinel node visualization and estimations of intra-nodal gadolinium concentration by the magnetic resonance imaging

Cited by 142 publications

References 51 publications

Development of a Macromolecular Dual-Modality MR-Optical Imaging for Sentinel Lymph Node Mapping

Development of a Macromolecular Dual-Modality MR-Optical Imaging for Sentinel Lymph Node Mapping

A Review of Techniques for Sentinel Lymph Node Mapping with Special Reference to the Head and Neck Region

Accurate sequential detection of primary tumor and metastatic lymphatics using a temperature-induced phase transition nanoparticulate system

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