Delpinium uncinatum mediated green synthesis of AgNPs and its antioxidant, enzyme inhibitory, cytotoxic and antimicrobial potentials

Abstract

Green synthesis of nanoparticles is becoming a method of choice for biological research due to its environmentally benign outcomes, stability and ease of synthesis. In this study, silver nanoparticles (AgNPs) were synthesized using stem (S-AgNPs), root (R-AgNPs) and mixture of stem and root (RS-AgNPs) of Delphinium uncinatum. The synthesized nanoparticles were characterized by standardized techniques and evaluated for their antioxidant, enzyme inhibition, cytotoxic and antimicrobial potentials. The AgNPs exhibited efficient antioxidant activities and considerable enzyme inhibition potential against alpha amylase, acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes. S-AgNPs showed strong cytotoxicity against human hepato-cellular carcinoma cells (HepG2) and high enzyme inhibitory effect (IC50 values 27.5μg/ml for AChE and 22.60 μg/ml for BChE) compared to R-AgNPs and RS-AgNPs. RS-AgNPs showed significant inhibition of Klebsiella pneumoniae and Aspergillus flavus and exhibited higher biocompatibility (<2% hemolysis) in human red blood cells hemolytic assays. The present study showed that biologically synthesized AgNPs using the extract of various parts of D. uncinatum have strong antioxidant and cytotoxic potentials.

α‐Fe2O3@Ag and Fe3O4@Ag Core‐Shell Nanoparticles: Green Synthesis, Magnetic Properties and Cytotoxic Performance

“…Silver nanoparticles can increase oxidative stress through the reduction of glutathione (GSH) and superoxide dismutase (SOD) and enlargement of lipid peroxidation for reaching the ultimate objective of apoptosis by intensifying caspase‐3 activity and DNA fragmentation. Ag nanoparticles and unbounded Ag ions have a tendency for forming interactions with thiol groups on the surface of GSH as well as reduced proteins similar to SOD, thioredoxin, and thioredoxin peroxidase [45] . Being comparable to the data of other reports, the present study denoted an extension in the rate of toxicity after amplifying the exerted concentration.…”

“…Ag nanoparticles and unbounded Ag ions have a tendency for forming interactions with thiol groups on the surface of GSH as well as reduced proteins similar to SOD, thioredoxin, and thioredoxin peroxidase. [45] Being comparable to the data of other reports, the present study denoted an extension in the rate of toxicity after amplifying the exerted concentration. Nonetheless, the performance of rigorous assessments can aid to achieve a sufficient conception on the true nature of the cytotoxicity of the synthesized nanoparticles.…”

Green synthesis of silver nanoparticles using Hebeloma excedens mushroom extract as a new source: Anti-lipid peroxidation, bioaccessibility and antidiabetic properties

“…For this reason, in recent increase in thermal resistance. Similar results have been demonstrated in many previous studies [56,57].…”

Medical Applications of Silver and Gold Nanoparticles and Core‐Shell Nanostructures Based on Silver or Gold Core: Recent Progress and Innovations

“…NPs obtained using a mixture of plant stem and root were characterized by the highest antifungal activity and significant inhibition of Aspergillus flavus. [118] In turn, Scroccarello et al tested the antifungal activity against Aspergillus niger of Ag NPs synthesized using various phenolic compounds, it turned out that all the compounds obtained had an inhibitory effect on mycelial growth, and the most effective were catechin-terminated NPs. [119] Research done by İpek et al confirmed the antipathogenic properties of Au NPs, in which an aqueous extract from the red skin of Allium cepa was used in the synthesis.…”