Delta-aminolevulinate dehydratase activity and oxidative stress markers in preeclampsia

Lucca, Leidiane de; Rodrigues, Fabiane; Jantsch, Letícia Bigolin; Kober, Helena; Neme, Walter Santos; Gallarreta, Francisco Maximiliano Pancich; Gonçalves, Thissiane L.

doi:10.1016/j.biopha.2016.09.033

Cited by 30 publications

(23 citation statements)

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“…Kirbas et al [36] observed significant decrease in the total antioxidant capacity in the blood of preeclamptic women. Similar associations concerning reduced SOD and catalase activities, and their mRNA levels have also been demonstrated in several other studies [26], [37], [38], [39], [40]. Although direct measurements of ROS in myometrial biopsies were not performed in our study due to its short lifespan, increases in PGC-1α and PGC-1β levels were observed, and may indicate oxidative stress [41].…”

Section: Discussionsupporting

confidence: 90%

Alterations in antioxidant system, mitochondrial biogenesis and autophagy in preeclamptic myometrium

et al. 2017

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Preeclampsia is a pregnancy complication which causes significant maternal and fetal morbidity and mortality worldwide. Although intensive research has been performed in the last 40 years, the pathology of preeclampsia is still poorly understood. The present work is a comparative study of the myometrium of women with normal pregnancy, and those with late- and early-onset preeclampsia (n = 10 for each group). We observed significant changes in the levels of antioxidant enzymes, markers of mitochondrial biogenesis and autophagy proteins in preeclamptic myometrium. Levels of superoxide dismutase 1 and catalase were lower in both preeclamptic groups than the control group. In late-onset preeclampsia, expression levels of essential mitochondria-related proteins VDAC1, TFAM, hexokinase 1, PGC-1α and PGC-1β, and autophagy marker LC3A, were significantly elevated. In the myometrium of the early-onset preeclampsia group OPA1 and Bcl-2 were up-regulated compared to those of the control (p < 0.05). These findings suggest that crucial molecular changes in the maternal myometrium occur with the development of preeclampsia.

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Section: Discussionsupporting

confidence: 90%

Alterations in antioxidant system, mitochondrial biogenesis and autophagy in preeclamptic myometrium

et al. 2017

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“…[ 4 ] Furthermore, placental vasoconstriction leads to a reduction of uteroplacental circulation that trigger the further release of inflammatory cytokines and anti-angiogenic factors which participate in the generation of a vicious cycle in the development of vascular endothelial dysfunction. [ 5 ]…”

Section: Introductionmentioning

confidence: 99%

Concept and connotation of oxidative stress in preeclampsia

2018

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BACKGROUND:Preeclampsia (PE) is a systemic pregnancy-related disorder characterized by hypertension, proteinuria, and edema. Free radicals seem to play an important role in the induction of endothelial dysfunction in PE.AIM:The aim of the present study was to investigate serum levels of nitric oxide (NO), peroxynitrite (ONOO−), paraoxonase (PON-1), malondialdehyde (MDA), and lipid profile in preeclamptic patients compared to the women with normal pregnancy.MATERIALS AND METHODS:A total of 68 pregnant women were recruited. They were divided into two groups - Group A, 40 women were a newly diagnosed with PE and Group B, 28 women with normal pregnancy. Anthropometric measurements including body mass index and blood pressure in accordance with biochemical measurements including NO, ONOO−, PON-1, MDA, and lipid profile were done for preeclamptic pregnant women compared to the controls.RESULTS:Pregnant women with pre-eclampsia illustrated insignificant differences in the age (31.22±2.87) compared to the age of control P > 0.05. There were significant changes in the body mass index (BMI), type of delivery and smoking status of pregnant women with pre-eclampsia compared to the control P < 0.05. Both systolic and diastolic blood pressures were high in pregnant women with pre-eclampsia compared to the control P < 0.01. PON-1 and NO serum levels were significantly decreased (P < 0.01) while ONOO− and MDA serum levels were significantly increased in PE compared to the women with normal pregnancy.CONCLUSIONS:This study concluded that PE is associated with the augmentation of oxidative stress and reduction of endogenous antioxidant capacity regarding PON-1.

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“…Como já se sabe, o mecanismo de dano do Pq ocorre pela formação de radicais livres e espécies reativas de oxigênio, demonstrando a importância da ação de antioxidantes na proteção do organismo em casos de intoxicação. A avaliação de biomarcadores relacionados ao estresse oxidativo, tais como TBARS, níveis de NPSH e atividade da enzima δ-ALA-D, é de extrema Tatiane Julia HAHN; Josiane Woutheres BORTOLOTTO; Thiago de Souza CLAUDINO; Gabriela BONFANTI-AZZOLIN importância para a determinação das características protetoras do flavonoide em estudo (ALMEIDA et al, 2016;DE LUCCA et al, 2016;KOROVILA et al, 2017;ANTUNES NETO et al, 2008).…”

Section: Resultsunclassified

“…A atividade da enzima δ-ALA-D pode ser dosada e utilizada como marcador indireto em situações de estresse oxidativo, sendo inibida por ter em sua estrutura grupamentos sulfidrila, os quais são sensíveis aos agentes oxidantes (DE LUCCA et al, 2016).…”

Section: Figura 1 -Níveis De Lipoperoxidaçãounclassified

Quercetina protege contra dano oxidativo causado por paraquat em fígado e rim de ratos

Hahn

Bortolotto

Claudino

et al. 2019

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As intoxicações por paraquat (Pq) são frequentes no país e seu mecanismo de ação tóxica envolve a geração de estresse oxidativo, que pode ser combatida com antioxidantes exógenos como a quercetina (Q). Assim, ao avaliar o papel desse flavonoide em dano causado por Pq em modelo experimental animal, in vitro, observou-se uma redução no nível de lipoperoxidação hepático, sugerindo maior sensibilidade desse tecido do que o renal. Também, quando em associação, Pq + Q foram capazes de provocar a inibição da enzima δ-aminolevulinato desidratase (δ-ALA-D), possivelmente por mecanismo de quelação, não relacionado aos grupos sulfidrílicos da enzima. In silico, a Q apresentou boa absorção intestinal, alta taxa de ligação às proteínas plasmáticas, potencial mutagênico, carcinogênico e inibitório sobre enzimas do metabolismo. Assim, acredita-se que a dieta alimentar rica em Q ou sua suplementação pode trazer benefícios à saúde da população, especialmente àqueles expostos a herbicidas, como o Pq.

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Delta-aminolevulinate dehydratase activity and oxidative stress markers in preeclampsia

Cited by 30 publications

References 36 publications

Alterations in antioxidant system, mitochondrial biogenesis and autophagy in preeclamptic myometrium

Alterations in antioxidant system, mitochondrial biogenesis and autophagy in preeclamptic myometrium

Concept and connotation of oxidative stress in preeclampsia

Quercetina protege contra dano oxidativo causado por paraquat em fígado e rim de ratos

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