Delta opioid receptor localization in the rat cerebellum

Abeyta, Antoinette; Dettmer, Todd; Barnes, Alicia; Vega, D.; Carta, Mario; Gallegos, Nalda Ludvina Cortés; Raymond-Stintz, Mary Ann; Savage, Daniel D.; Valenzuela, C. Fernando; Saland, L.C.

doi:10.1016/s0006-8993(02)02248-5

Cited by 22 publications

(9 citation statements)

References 28 publications

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“…Recently, the existence of significant expression of the DOR in the rat cerebellum was established (Abeyta et al, 2002). The levels of DOR proteins in cerebellum of normal rat were low but were significantly higher in Hx rats.…”

Section: Discussionmentioning

confidence: 99%

“…This finding correlates well with the location of DOR mRNA (Jenab et al, 1995; Mansour et al, 1995). Recently, it was shown that stimulation of DORs in frozen sections of rat brain increased binding of [ 35 S]GTPγS in the cerebellar stratum moleculare (Abeyta et al, 2002). An immunoreactive 48 kDa DOR protein band was found in all investigated brain regions, including Purkinje cells and the granular cell layer of the cerebellum (Abeyta et al, 2002).…”

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confidence: 99%

“…By using normal, hypophysectomized (Hx) rats receiving cortisol (C; 400 μg/kg/day) and L‐thyroxine (T 4 ; 10 μg/kg/day; T 4 /C), and Hx rats also given bovine GH (bGH; 1 mg/kg body weight; T 4 /C/GH) for 19 days as daily subcutaneous injections, we wanted to determine whether GH regulates the DOR expression. The cerebral cortex and the cerebellum were investigated, because these areas may possess differences in expression of DOR subtypes and because the content of DOR protein and DOR mRNA has been shown to be significantly different in these areas (Mansour et al, 1995; Vathy et al, 2000; Abeyta et al, 2002).…”

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confidence: 99%

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Expression of delta opioid receptor mRNA and protein in the rat cerebral cortex and cerebellum is decreased by growth hormone

Persson

Åberg

Oscarsson

et al. 2002

J of Neuroscience Research

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Hormones released from the pituitary have been shown to regulate the expression of different proteins in the central nervous system. We wanted to examine whether peripheral administration of bovine growth hormone (bGH) regulates the expression of delta-opioid receptor (DOR) in the cerebral cortex and cerebellum. Expression of the DOR protein was quantified using Western blot densitometry. DOR mRNA was quantified with a solution hybridization RNase protection assay. Hypophysectomized (Hx) and untreated normal female rats were included in the study. All Hx rats were hormonally treated with cortisol (400 microg/kg/day) and L-thyroxine (10 microg/kg/day) for 19 days. Hypophysectomy resulted in a threefold increase in cerebral cortex and a twofold increase in cerebellum of the DOR protein compared with normal rats. One subgroup of Hx rats received bGH (1 mg/kg body weight) as a daily subcutaneous injection for 19 days. This treatment normalized the levels of DOR protein in the cerebral cortex and cerebellum. Immunohistochemical experiments showed that GH decreased DOR expression especially in layers II-VI in cerebral cortex and in stratum moleculare in cerebellum. Quantification of DOR mRNA by solution hybridization RNase protection assay corresponded to the DOR protein measurements. We conclude that the expression of DORs in cerebral cortex and cerebellum is regulated by GH.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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Expression of delta opioid receptor mRNA and protein in the rat cerebral cortex and cerebellum is decreased by growth hormone

Persson

Åberg

Oscarsson

et al. 2002

J of Neuroscience Research

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“…The greatest δ-OR densities are found in the olfactory bulb, caudate-putamen, hypothalamus and neocortex. Thalamus, nucleus accumbens and brainstem have moderate δ-OR distribution [27] and small levels of δ-OR have been also demonstrated in the cerebellum [28].…”

Section: Anatomical Distribution Of Opioid Receptorsmentioning

confidence: 99%

Expression of Opioid Receptors During Peripheral Inflammation

Pol¹,

Puig²

2004

CTMC

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Opioid receptors (OR) and their mRNA are present in the central and peripheral nervous system of mammals. In this review we examine the behavioral effects of opioids and the expression of their receptors during peripheral inflammation in two experimental models: the rat paw and the mouse intestine. Inflammation increased the antinociceptive (paw) and the inhibitory effects of opioids in the gut (transit, permeability and plasma extravasation) by interaction with OR located at peripheral sites. Based on agonist efficacy, micro > delta >> kappa-OR mediate the antinociceptive and antitransit effects of opioids during inflammation. Intestinal permeability is modulated by delta = micro >> kappa-OR, while kappa > delta >> micro-OR are involved in the inhibition of plasma extravasation. Intestinal inflammation increased the transcription of micro and delta-OR (but not kappa) genes in the gut, thus explaining the enhanced antitransit and antisecretory effects of micro and delta-OR agonists; however, the increased inhibitory effects of kappa-OR agonists on plasma extravasation could result from post-transcriptional regulation of the receptor. Similarly, the increased expression of peripheral micro-OR observed in the rat paw during inflammation, occurs at post-transcriptional levels and is related to an increased axonal transport from the dorsal root ganglia to peripheral terminals. The sites and mechanisms implicated in the increased transcription of micro and delta-OR during intestinal inflammation are under investigation.

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“…Upon maturation of the brain region, the cells may be reduced or disappear as seen for rodent cerebellar opioid receptors [1,11,29]. Programmed neuronal cell death that occurs during development may be responsible for this loss.…”

Section: Introductionmentioning

confidence: 99%

Increased opioid receptor binding and G protein coupling in the accumbens and ventral tegmental area of postnatal day 2 rats

Yan-ning

Belcheva

Clark

et al. 2006

Neuroscience Letters

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In some regions of the developing rat brain such as the nucleus accumbens (Acb), mu opioid (MOP) receptor specific binding in the perinatal period exceeds that in the adult. To investigate the significance of these developmental changes, MOP and nociceptin/orphanin FQ (NOP) receptor binding and G protein coupling as determined by GTPγS binding experiments were examined in mesolimbic regions of postnatal day 2 (P2) pups and compared to those of their dams. Acb of the P2 pup exhibited 2-fold greater MOP receptor specific binding than that of the dam. In the ventral tegmental area (VTA), NOP specific binding was about 2-fold higher in the P2 pup. A correlation was found between MOP and NOP binding and their coupling to G protein on dam and P2 pup brain sections. However, the magnitude of increases in MOP and NOP receptor G protein coupling to G protein in P2 pups exceeded the 2-fold differences in binding between pups and dams. Furthermore, the amplitude of the MOP receptor G protein coupling in female P2 Acb was greater than increases in male P2 pup Acb. Differences in MOP and NOP binding and G protein coupling in other mesolimbic regions between P2 pups and dams were rarely observed. The data indicate that greater binding and G protein coupling of MOP and NOP receptors occur in discrete, mesolimbic regions of P2 pups when compared to their dams. It may be of significance that these brain regions, Acb and VTA, are undergoing maturation on P2. KeywordsDevelopment; Nociceptin; Mu opioid receptor; Mesolimbic region; Cortex; Nociceptin receptor Opioid receptors and their endogenous ligands have been detected in rodent brain beginning as early as the second week of gestation [4,5,10,15,21,22,25,26]. In experiments where forebrain opioid receptor binding densities were measured by in vitro binding assays, it was determined that the number of opioid receptor binding sites increases with age. When selective ligands were used in such studies, a differential ontogeny for μ (MOP), δ (DOP) and κ (KOP) receptors was discovered. Forebrain MOP and KOP binding was detectable in the prenatal period, whereas DOP binding was detectable only in the second postnatal week [10,15,22,25,26,28]. Interestingly, when MOP binding density in rodent forebrain was measured with respect to protein instead of wet tissue weight, its binding density was higher in the prenatal interval [15,22], but declined in the postnatal period [25,26] [11,29]. In support of this possibility, DAMGO stimulation of GTPγS binding has been detected in mouse brain as early as embryonic day 12.5, a time at which MOP receptor is also detected [19,22].The newest member of the opioid receptor family is the NOP receptor. NOP receptor binding and G protein coupling are abundant in the cerebral cortex and some limbic regions of the adult rodent and primate brain as shown by autoradiographic methods [3,6,9,13,23,24]. Although the ontogeny of NOP receptor mRNA expression has been studied [9,17], its binding and G protein coupling capacity in brain during development ha...

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Delta opioid receptor localization in the rat cerebellum

Cited by 22 publications

References 28 publications

Expression of delta opioid receptor mRNA and protein in the rat cerebral cortex and cerebellum is decreased by growth hormone

Expression of delta opioid receptor mRNA and protein in the rat cerebral cortex and cerebellum is decreased by growth hormone

Expression of Opioid Receptors During Peripheral Inflammation

Increased opioid receptor binding and G protein coupling in the accumbens and ventral tegmental area of postnatal day 2 rats

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