Demethoxycurcumin Suppresses Proliferation, Migration, and Invasion of Human Brain Glioblastoma Multiforme GBM 8401 Cells <i>via</i> PI3K/Akt Pathway

Hsueh, Shu‐Ching; Chen, Cheng‐Yen; Hsu, Ming-Jie; Lu, Hsu‐Feng; Peng, Shu‐Fen; Chen, Po‐Yuan; Lien, Jin‐Cherng; Chen, Yung‐Liang; Chueh, Fu‐Shin; Chung, Jing‐Gung; Yeh, Ming‐Yang; Huang, Yiping

doi:10.21873/anticanres.14952

Cited by 10 publications

(6 citation statements)

References 56 publications

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“…These results indicated that PEITC affected the NF-κB signaling pathway and may affect the aggressiveness of glioblastoma and the inflammatory microenvironment. In our previous study, demethoxycurcumin inhibited the motility, migration, and invasion of GBM 8401 cells via inhibition of PI3K/Akt and NF-κB signaling pathways [46]. PEITC reduced migration and invasion through the inhibition of uPA, Rho A, and Ras with inhibition of matrix metalloproteinase gene expression in GBM 8401 cells [13].…”

Section: Discussionmentioning

confidence: 90%

Phenethyl Isothiocyanate Suppresses the Proinflammatory Cytokines in Human Glioblastoma Cells through the PI3K/Akt/NF-κB Signaling Pathway In Vitro

Hsu

Lee

Liu

et al. 2022

Oxidative Medicine and Cellular Longevity

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Phenethyl isothiocyanate (PEITC), extracted from cruciferous vegetables, showed anticancer activity in many human cancer cells. Our previous studies disclosed the anticancer activity of PEITC in human glioblastoma multiforme (GBM) 8401 cells, including suppressing the cell proliferation, inducing apoptotic cell death, and suppressing cell migration and invasion. Furthermore, PEITC also inhibited the growth of xenograft tumors of human glioblastoma cells. We are the first to investigate PEITC effects on the receptor tyrosine kinase (RTK) signaling pathway and the effects of proinflammatory cytokines on glioblastoma. The cell viability was analyzed by flow cytometric assay. The protein levels and mRNA expressions of cytokines, including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6), were determined by enzyme-linked immunosorbent assay (ELISA) reader and real-time polymerase chain reaction (PCR) analysis, respectively. Furthermore, nuclear factor-kappa B- (NF-κB-) associated proteins were evaluated by western blotting. NF-κB expression and nuclear translocation were confirmed by confocal laser microscopy. NF-κB binding to the DNA was examined by electrophoretic mobility shift assay (EMSA). Our results indicated that PEITC decreased the cell viability and inhibited the protein levels and expressions of IL-1β, IL-6, and TNF-α genes at the transcriptional level in GBM 8401 cells. PEITC inhibited the binding of NF-κB on promoter site of DNA in GBM 8401 cells. PEITC also altered the protein expressions of protein kinase B (Akt), extracellular signal-regulated kinase (ERK), and NF-κB signaling pathways. The inflammatory responses in human glioblastoma cells may be suppressed by PEITC through the phosphoinositide 3-kinase (PI3K)/Akt/NF-κB signaling pathway. Thus, PEITC may have the potential to be an anti-inflammatory agent for human glioblastoma in the future.

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Section: Discussionmentioning

confidence: 90%

Phenethyl Isothiocyanate Suppresses the Proinflammatory Cytokines in Human Glioblastoma Cells through the PI3K/Akt/NF-κB Signaling Pathway In Vitro

Hsu

Lee

Liu

et al. 2022

Oxidative Medicine and Cellular Longevity

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“…Liu et al found that β-elemene enhanced the radiosensitivity and chemical sensitivity of glioblastoma cells by inhibiting the ataxia telangiectasia mutated signaling pathway [ 26 ]. Su et al reported that demethoxycurcumin prevented the proliferation, migration and invasion of human glioblastoma multiforme glioma cells [ 29 ]. However, the effects of turmeric volatile oil on glioma have not yet been reported.…”

Section: Discussionmentioning

confidence: 99%

Ar-turmerone inhibits the proliferation and mobility of glioma by downregulating cathepsin B

Cao,

Chen,

Xiao

et al. 2023

Aging

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Ar-turmerone, a compound isolated from turmeric seeds, has exhibited anti-malignant, anti-aging and anti-inflammatory properties. Here, we assessed the effects of ar-turmerone on glioma cells. U251, U87 and LN229 glioma cell lines were treated with different concentrations of ar-turmerone (0, 50, 100 and 200 μM), and their viability and mobility were evaluated using Cell Counting Kit 8, colony formation, wound healing and Transwell assays. The effects of ar-turmerone on U251 glioma cell proliferation were also assessed using a subcutaneous implantation tumor model. High-throughput sequencing, bioinformatic analyses and quantitative real-time polymerase chain reactions were used to identify the key signaling pathways and targets of ar-turmerone. Ar-turmerone reduced the proliferation rate and mobility of glioma cells in vitro and arrested cell division at G1/S phase. Cathepsin B was identified as a key target of ar-turmerone in glioma cells. Ar-turmerone treatment reduced cathepsin B expression and inhibited the cleavage of its target protein P27 in glioma cells. On the other hand, cathepsin B overexpression reversed the inhibitory effects of ar-turmerone on glioma cell proliferation, mobility progression in vitro and in vivo . In conclusion, ar-turmerone suppressed cathepsin B expression and P27 cleavage, thereby inhibiting the proliferation and mobility of glioma cells.

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“…Studies have confirmed that curcumin and a variety of curcumin compounds promote the early apoptosis of glioma cells, inhibit their growth and migration, induce DNA damage, and have been identified as potential anti-glioma drugs [ 22 , 23 ]. For example, Majchrzak et al showed that sodium butyrate increased the permeability of curcumin through the blood–brain barrier, restored the expression of Wnt/β-catenin pathway antagonist genes, and reduced the vitality of glioblastoma cells [ 24 ]. Liu et al found that β-elemene enhanced the radiosensitivity and chemical sensitivity of glioblastoma cells by inhibiting ATM signaling pathways [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Terpinen-4-ol Induces Ferroptosis of Glioma Cells via Downregulating JUN Proto-Oncogene

Cao

Zeng

et al. 2023

Molecules

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According to previous research, turmeric seeds exhibit anti-inflammatory, anti-malignancy, and anti-aging properties due to an abundance of terpinen-4-ol (T4O). Although it is still unclear how T4O works on glioma cells, limited data exist regarding its specific effects. In order to determine whether or not glioma cell lines U251, U87, and LN229 are viable, CCK8 was used as an assay and a colony formation assay was performed using different concentrations of T4O (0, 1, 2, and 4 μM). The effect of T4O on the proliferation of glioma cell line U251 was detected through the subcutaneous implantation of the tumor model. Through high-throughput sequencing, a bioinformatic analysis, and real-time quantitative polymerase chain reactions, we identified the key signaling pathways and targets of T4O. Finally, for the measurement of the cellular ferroptosis levels, we examined the relationship between T4O, ferroptosis, and JUN and the malignant biological properties of glioma cells. T4O significantly inhibited glioma cell growth and colony formation and induced ferroptosis in the glioma cells. T4O inhibited the subcutaneous tumor proliferation of the glioma cells in vivo. T4O suppressed JUN transcription and significantly reduced its expression in the glioma cells. The T4O treatment inhibited GPX4 transcription through JUN. The overexpression of JUN suppressed ferroptosis in the cells rescued through T4O treatment. Taken together, our data suggest that the natural product T4O exerts its anti-cancer effects by inducing JUN/GPX4-dependent ferroptosis and inhibiting cell proliferation, and T4O will hope-fully serve as a prospective compound for glioma treatment.

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Demethoxycurcumin Suppresses Proliferation, Migration, and Invasion of Human Brain Glioblastoma Multiforme GBM 8401 Cells via PI3K/Akt Pathway

Cited by 10 publications

References 56 publications

Phenethyl Isothiocyanate Suppresses the Proinflammatory Cytokines in Human Glioblastoma Cells through the PI3K/Akt/NF-κB Signaling Pathway In Vitro

Phenethyl Isothiocyanate Suppresses the Proinflammatory Cytokines in Human Glioblastoma Cells through the PI3K/Akt/NF-κB Signaling Pathway In Vitro

Ar-turmerone inhibits the proliferation and mobility of glioma by downregulating cathepsin B

Terpinen-4-ol Induces Ferroptosis of Glioma Cells via Downregulating JUN Proto-Oncogene

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