Demethylation effects of elemene on the GSTP1 gene in HCC cell line QGY7703

Wu, Bingcheng; Jiang, Yong; Zhu, Feng; Sun, Donglin; Huang, Hui

doi:10.3892/ol.2016.4243

Cited by 9 publications

(8 citation statements)

References 33 publications

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“…29 Methylation of the GSTP1 gene CpG island promoter is involved in the occurrence and development of multiple tumors, which could be a biomarker for early screen, diagnosis and prognosis. 30 Studies have emphasized the ability of GSTP1 expression is an important predictor of the clinical outcome or drug resistance to ESCC. Lymph node metastasis, and overexpression of GSTP1 were all determined to be independent predictors linked to poor prognoses, with this association tightly correlated with the chemotherapeutic efficacy of 5-FU combined with cisplatin in ESCC patients.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of long noncoding RNA LINC01419 in esophageal squamous cell carcinoma and its relation to the sensitivity to 5-fluorouracil by mediating GSTP1 methylation

et al. 2019

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Background: Genome-wide sequencing investigations have identified numerous long noncoding RNAs (lncRNAs) among mammals, many of which exhibit aberrant expression in cancers, including esophageal squamous cell carcinoma (ESCC). Herein, this study elucidates the role and mechanism by which LINC01419 regulates the DNA methylation of glutathione S-transferase pi 1 (GSTP1) in relation to ESCC progression and the sensitivity of ESCC cells to 5-fluorouracil (5-FU). Methods: LINC01419 and GSTP1 levels were quantified among 38 paired ESCC and adjacent tissue samples collected from patients with ESCC. To ascertain the contributory role of LINC01419 in the progression of ESCC and identify the interaction between LINC01419 and GSTP1 promoter methylation, LINC01419 was overexpressed or silenced, and the DNA methyltransferase inhibitor 5-Aza-CdR was treated. Results: Data from the GEO database (GSE21362) and the Cancer Genome Atlas displayed elevated levels of LINC01419 and downregulated levels of GSTP1 in the ESCC tissues and cells. The silencing of LINC01419 led to decreased proliferation, increased apoptosis, and enhanced sensitivity to 5-FU in ESCC cells. Notably, LINC01419 could bind to the promoter region of the GSTP1 gene, resulting in elevated GSTP1 methylation and reduced GSTP1 levels via the recruitment of DNA methyltransferase among ESCC cells, whereby ESCC progression was stimulated accompanied by reduced ESCC cell sensitivity to 5-FU. GSTP1 demethylation by 5-Aza-CdR was observed to reverse the effects of LINC01419 overexpression in ESCC cells and the response to 5-FU. Conclusion: Highly expressed LINC01419 in ESCC promotes GSTP1 methylation, which ultimately acts to promote the event of ESCC and diminish the sensitivity of ESCC cells to 5-FU, highlighting a novel potential strategy to improve 5-FU-based chemotherapy in ESCC.

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Section: Discussionmentioning

confidence: 99%

Overexpression of long noncoding RNA LINC01419 in esophageal squamous cell carcinoma and its relation to the sensitivity to 5-fluorouracil by mediating GSTP1 methylation

et al. 2019

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“…It has been found that bELE is a potent anticancer agent against multiple cancers in extensive clinical trials and experimental research in vivo and in vitro. Wu et al found that bELE increased apoptosis and caused S phase arrest of several hepatocellular carcinoma cell lines in a dose‐dependent manner []. In lung cancer, peroxiredoxin‐1, a critical molecule in redox regulation, has been identified and confirmed as a downstream effector suppressed by bELE [].…”

Section: Discussionmentioning

confidence: 99%

β-Elemene Selectively Inhibits the Proliferation of Glioma Stem-Like Cells Through the Downregulation of Notch1

Feng

Wang

Jiang

et al. 2016

Stem Cells Translational Medicine

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Glioma is the most frequent primary central nervous system tumor. Although the current first‐line medicine, temozolomide (TMZ), promotes patient survival, drug resistance develops easily. Thus, it is important to investigate novel therapeutic reagents to solidify the treatment effect. β‐Elemene (bELE) is a compound from a Chinese herb whose anticancer effect has been shown in various types of cancer. However, its role in the inhibition of glioma stem‐like cells (GSLCs) has not yet been reported. We studied both the in vitro and the in vivo inhibitory effect of bELE and TMZ in GSLCs and parental cells and their combined effects. The molecular mechanisms were also investigated. We also optimized the delivery methods of bELE. We found that bELE selectively inhibits the proliferation and sphere formation of GSLCs, other than parental glioma cells, and TMZ exerts its effects on parental cells instead of GSLCs. The in vivo data confirmed that the combination of bELE and TMZ worked better in the xenografts of GSLCs, mimicking the situation of tumorigenesis of human cancer. Notch1 was downregulated with bELE treatment. Our data also demonstrated that the continuous administration of bELE produces an ideal effect to control tumor progression. Our findings have demonstrated, for the first time, that bELE could compensate for TMZ to kill both GSLCs and nonstem‐like cancer cells, probably improving the prognosis of glioma patients tremendously. Notch1 might be a downstream target of bELE. Therefore, our data shed light on improving the outcomes of glioma patients by combining bELE and TMZ. Stem Cells Translational Medicine 2017;6:830–839

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“…Hypermethylation associated with SOCS protein silencing has been found in a variety of cancers, including myeloma, melanoma, bladder cancer, liver cancer, gastric cancer, and colorectal cancer. [ 30 ] The effect of risk factors on DNA methylation in HCC needed to be analyzed. Therefore, this study analyzed the effects of sex on the relationship between DNA methylation and HCC.…”

Section: Discussionmentioning

confidence: 99%

“…The incidence rate of HCC in men was 4 to 8 times higher than that in women. Wu et al [ 30 ] reported that the methylation of the SOCS3 gene was high in HCC and had no significant correlation with the patient's sex, age, tumor size, and differentiation degree. Li et al [ 31 ] showed that the expression of SOCS3 positively correlated with the prevalence of HCC, but not with the age and sex of patients with HCC.…”

Section: Discussionmentioning

confidence: 99%

Association between SOCS3 hypermethylation and HBV-related hepatocellular carcinoma and effect of sex and age

et al. 2021

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Background: Suppressor 3 of cytokine signaling (SOCS3) hypermethylation has been reported to participate in hepatocellular carcinoma (HCC) development and progression, but conflicting results were published. This study aimed to analyze the clinical effects of SOCS3 hypermethylation in HCC and the effects of sex and age on SOCS3 hypermethylation in HCC.Methods: Databases were searched for relevant case-control and cohort studies on SOCS3 hypermethylation in HBV-related HCC. In vitro and in vivo studies and studies of patients with serious comorbidities were excluded. Review Manager 5.2 was used to estimate the effects of the results among the selected studies. Forest plots, sensitivity analysis, and bias analysis for the included studies were also conducted.Results: Finally, 8 relevant studies met the inclusion criteria. A significant difference in SOCS3 hypermethylation in HCC was found between tumor and nontumor groups (the odds ratio [OR] = 2.01, 95% confidence interval [CI]: 1.48-2.73, P < .00001; P for heterogeneity = .39, I 2 = 5%). The meta-analysis suggested no significant difference in the effect of sex (OR = 1.00, 95% CI: 0.76-1.31, P = .76; P for heterogeneity = .44, I 2 = 0%) and age on SOCS3 hypermethylation in HCC (OR = 1.11, 100% CI: 0.78-1.29, P = .03; P for heterogeneity = .14, I 2 = 36%). Limited publication bias was observed in this study.Conclusion: SOCS3 hypermethylation is associated with HBV-related HCC. Sex and age do not affect the association between SOCS3 hypermethylation and HCC. SOCS3 might be a treatment target for HCC.Abbreviations: CIs = confidence intervals, HBV = hepatitis B virus, HCC = hepatocellular carcinoma, NOS = Newcastle-Ottawa Scale, OR = odds ratio, SOCS = suppressors of cytokine signaling, SOCS3 = suppressor 3 of cytokine signaling.

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Demethylation effects of elemene on the GSTP1 gene in HCC cell line QGY7703

Cited by 9 publications

References 33 publications

Overexpression of long noncoding RNA LINC01419 in esophageal squamous cell carcinoma and its relation to the sensitivity to 5-fluorouracil by mediating GSTP1 methylation

Overexpression of long noncoding RNA LINC01419 in esophageal squamous cell carcinoma and its relation to the sensitivity to 5-fluorouracil by mediating GSTP1 methylation

β-Elemene Selectively Inhibits the Proliferation of Glioma Stem-Like Cells Through the Downregulation of Notch1

Association between SOCS3 hypermethylation and HBV-related hepatocellular carcinoma and effect of sex and age

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