Demonstration by Plaque Reduction Technique of Immunologic Relationship between Canine Herpes Virus and Herpes Simplex Virus

Aurelian, L

doi:10.3181/00379727-127-32721

Cited by 16 publications

(9 citation statements)

References 2 publications

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“…Transfections were done as described (Knipe et al, 1979) except that cells were overlaid with MEM with 2% FCS and incubation was at 34 °C. When generalized c.p.e, was observed, virus was harvested and plaque-purified under a methyl cellulose overlay (Aurelian, 1968) at 34 °C. Virus from individual plaques was grown for one passage in HEp-2 ceils prior to titration at 34 oC and 39 °C.…”

Section: Preparation Of Radiolabelled Cell Extracts and Immunoprecipimentioning

confidence: 99%

Expression of the large subunit of herpes simplex virus type 2 ribonucleotide reductase (ICP10) is required for virus growth and neoplastic transformation

Smith

Kulka

Wymer

et al. 1992

Journal of General Virology

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The amino-terminal domain of the large subunit of herpes simplex virus type 2 (HSV-2) ribonucleotide reductase (ICP 10) has protein kinase (PK) activity and properties similar to those of growth factor receptor kinases which can be activated to transforming potential. DNA sequences that encode the PK domain cause neoplastic transformation of immortalized cells. The studies described in this report used a spontaneous mutant (ts5-152) temperature-sensitive for the synthesis of 1CPI0 and the previously described ICP10 expression vectors to study the role of ICP10 expression in HSV-2 growth and neoplastic potential. The titres of the ts5-152 mutant are 1000-fold lower at 39 °C compared to 34 °C after 12 h post-infection. The efficiency of plaquing is 0-003. The growth defect at 39 °C correlates with decreased ICP10 synthesis. Sequence analysis of the PK domain of the ts5-152 ICP10 gene identified a pair of frameshift mutations resulting in a 19 amino acid residue substitution at positions 275 to 293 and a downstream single base pair mutation causing a substitution at position 309. Cloning of the mutant ICP 10 gene from tsS-152 into a wild-type HSV-2 isolate resulted in a recombinant (859/152) with growth properties and rates of ICP10 synthesis at 39 °C similar to those of ts5-152. Cells transformed with u.v.-inactivated ts5-152, or the recombinant 859/152, have significantly decreased cloning efficiency in agarose at 39 °C, but only during the first 250 post-transfer population doublings. Anchorage-independent growth was observed in cells transfected with expression vectors pJWl 7 or pJW32 that express ICP10 or its PK domain, respectively. Cells transfected with the frameshift mutant pJW21 or the ICP10 carboxy-terminal vector pJW31 did not form clones in agarose.

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Section: Preparation Of Radiolabelled Cell Extracts and Immunoprecipimentioning

confidence: 99%

Expression of the large subunit of herpes simplex virus type 2 ribonucleotide reductase (ICP10) is required for virus growth and neoplastic transformation

Smith

Kulka

Wymer

et al. 1992

Journal of General Virology

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“…Antiserum to HSV-1 contains both specific and crossreacting neutralizing antibodies (Sire & Watson, 1973). The latter react with several herpesviruses including HSV-2, bovine mammillitis virus (BMV), herpesvirus B, herpesvirus tamarinus and, possibly, canine herpesvirus (Watson et al, 1967;Hampar et al, 1969;Blue & Plummer, 1973;Aurelian, 1968). The identification of which glycoprotein induces which particular type of antibody is being investigated by purification of individual glycoproteins for the production of monoprecipitin antiserum (Eberle & Courtney, 1980 a) and by the production of monoclonal antibodies to HSV glycoproteins.…”

Section: Introductionmentioning

confidence: 99%

Antigenic and Biochemical Analysis of gB of Herpes Simplex Virus Type 1 and Type 2 and of Cross-reacting Glycoproteins Induced by Bovine Mammillitis Virus and Equine Herpesvirus Type 1

Snowden

Kinchington

Powell

et al. 1985

Journal of General Virology

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SUMMARYAn antiserum was produced to the oligomeric form ofglycoprotein B (gB) induced by herpes simplex virus type 1 (HSV-1) strain 17. This antiserum gave a single common precipitin line in agar gel immunodiffusion with HSV-I, HSV-2, bovine mammillitis virus (BMV) and equine herpesvirus type 1 (EHV-1). It also neutralized HSV-1, HSV-2 and BMV but not EHV-1. Absorption of the antiserum with excess HSV-2 or BMV antigen resulted in an HSV-l-specific neutralizing antiserum. In immunoprecipitation, two proteins, gB and pgB, were precipitated from HSV-1-and HSV-2-infected cells and at least three from BMV-and EHV-l-infected cells. Glycoprotein B and pgB of three HSV-1 and three HSV-2 strains and the corresponding antigenically related glycoproteins of BMV-and EHV-l-infected cells were labelled with l:s I, digested with trypsin and the resulting peptides separated by two-dimensional thin-layer chromatography or high-pressure liquid chromatography. The resulting profiles were found to be almost identical, suggesting considerable structural conservation of the peptide backbone of the antigenically related glycoproteins of these four viruses.

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“…However, there is no 1.70 < simple explanation for the observation that, in -1.68 cells treated with puromycin after the onset of viral DNA synthesis, the rate of incorporation of thymidine into viral DNA is reduced. The greatest reduction in rate of incorporation of thymidine into viral DNA occurs within a short period of time after addition of puromycin, and could reflect either a sudden change in the pool 1.74 size of thymidine or a real change in the rate of -1.72 < synthesis of viral DNA. The first explanation must be considered in view of the possibility 1.70raised by Newton et al (12) that the size of the -1.68 cmthymidine pool in the infected cells may change during the reproductive cycle, and the observation made in this study that the rate of uptake of thymidine into cellular DNA increases during 4 and 10 hr after infection.…”

Section: Discussionmentioning

confidence: 99%

“…Virus. The pertinent properties of the F205 strain of CHV have been described (1,2,6,20). Virus assays were performed by a method previously described (1), and titers are expressed in terms of plaque-forming units (PFU)/ml of fluid.…”

Section: Materials and Methods Solutions And Media Pbs-a Consisted Omentioning

confidence: 99%

Replication of Canine Herpesvirus

Aurelian

1969

J Virol

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This paper reports the results of two series of experiments. The first series indicated that deoxyribonucleic acid (DNA) extracted from partially purified canine herpesvirus virions is characterized by a high guanine plus cytosine molar base ratio (65 to 67 mole%), similar to the DNA of herpes simplex virus. In the second series of experiments it was estimated, on the basis of uptake of tritiated thymidine, that in dog kidney cells canine herpesvirus-DNA synthesis starts at 4 hr and continues until 16 hr after infection. Treatment of infected cells with puromycin during the first 4 hr of infection blocks the onset of viral DNA synthesis, whereas, after this time the uptake of thymidine is unaffected. Recently, a virus morphologically similar to the

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Demonstration by Plaque Reduction Technique of Immunologic Relationship between Canine Herpes Virus and Herpes Simplex Virus

Cited by 16 publications

References 2 publications

Expression of the large subunit of herpes simplex virus type 2 ribonucleotide reductase (ICP10) is required for virus growth and neoplastic transformation

Expression of the large subunit of herpes simplex virus type 2 ribonucleotide reductase (ICP10) is required for virus growth and neoplastic transformation

Antigenic and Biochemical Analysis of gB of Herpes Simplex Virus Type 1 and Type 2 and of Cross-reacting Glycoproteins Induced by Bovine Mammillitis Virus and Equine Herpesvirus Type 1

Replication of Canine Herpesvirus

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