Demonstration of concurrent dengue 1 and dengue 3 infection in six patients by the polymerase chain reaction

Abstract

A dual viremia resulting from naturally acquired dengue 1 and dengue 3 infections in six patients experiencing a dengue-like syndrome during the epidemic in New Caledonia in 1989 is reported. Serotype identification was first based on virus isolation in mosquito cells and immunofluorescence using type-specific monoclonal antibodies. The double infection was confirmed directly in blood samples by the polymerase chain reaction (PCR) on genomic RNA and hybridization of the amplified cDNA fragments with type-specific DNA probes.

Applications of Polymerase Chain Reaction for Identification of Dengue Viruses Isolated from Patient Sera

“…In spite of the fact that this question can be verified by staining with 1F 1 MAb, unfortunately the sample was insufficient to be tested. Taken together, dual infection of two serotypes of dengue viruses (6,12) is not uncommon in the area where two or more dengue virus serotypes are co-circulated during the epidemic. The prevalence of dual infection should be investigated further by performing a direct RT-PCR of the original serum samples rather than preamplifying the viruses in the mosquito or mosquito cell line because one strain of virus may be selectively lost during the process of virus isolation.…”

“…Moreover, a case of natural human infection with DEN-1 and DEN-4 viruses has also been demonstrated during the 1982 outbreak in Puerto Rico (6). Recently, Laille et al (12) have shown an evidence of concurrent infection with DEN-1 and DEN-3 by polymerase chain reaction in six patients during the epidemic in New Caledonia in 1989. In Thailand, it is well established that there are multiple serotypes of dengue viruses circulating simultaneously in the same area and at least 3 serotypes have been isolated each year during the outbreaks (from 1962 to 1988) in Bangkok (21).…”

Diagnosis of Dengue by Using Reverse Transcriptase-Polymerase Chain Reaction

“…albopictus C6/36 cell line have been routinely used for dengue diagnosis in Brazil (Schatzmayr et al 1986, Figueiredo et al 1992, Vasconcelos et al 1993). However, due to its speed and sensitivity, PCR has been adopted as an alternative method in dengue diagnosis (Laille et al 1991, Chungue et al 1993, Morita et al 1994.…”

Concurrent infection with dengue virus type-2 and DENV-3 in a patient from Ceará, Brazil

“…In March 2002, DENV-3 was first isolated in Ceará by the Central Laboratory of Public Heath, thus it became an area of high endemicity with three cocirculating serotypes. Interestingly, in spite of the high or hyperendemicity of dengue viruses in some populations, relatively few cases of double infection have been reported (Gubler et al 1985, Laille et al 1991, Maneekarn et al 1993, Sisouk et al 1995. More recently two cases were reported in Brazil involving DENV-1 DENV-2 (Santos et al 2003).…”