Demonstration of Epitope Spreading in Bullous Pemphigoid: Results of a Prospective Multicenter Study

Hashimoto, Takashi; Tsuruta, Daisuke; Dainichi, Teruki; Hamada, Takahiro; Furumura, Minao; Ishii, Norito

doi:10.1038/jid.2011.276

Cited by 15 publications

(13 citation statements)

References 7 publications

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“…Therefore, it is possible that antiperiplakin antibody may be produced by the epitope-spreading mechanism. 29 The appearance of the 190 kDa band did not correspond with the clinical severity score (see Table 2). It is possible that the presence of the 190 kDa protein band does not have any clinical significance.…”

Section: Discussionmentioning

confidence: 97%

Clinical and Immunologic Characterization in 26 Indian Pemphigus Patients

et al. 2013

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Background: Pemphigus shows geographically variable characteristics.Objective: To study the clinical and immunologic characteristics of Indian pemphigus patients before and after treatment. Methods: Twenty-six Indian pemphigus patients were analyzed with regard to age, gender, clinical features, treatments and response, the results of histopathology, direct and indirect immunofluorescence (IF), enzyme-linked immunosorbent assay (ELISA), and immunoblot analyses.Results: There were 22 pemphigus vulgaris (PV) and 4 pemphigus foliaceus (PF) patients. Direct and indirect IF was positive in 95.8% and 56% of patients, respectively. Indices of ELISA were lower in our study. Immunoblot assays detected the 130 kDa desmoglein-3 in 10 PV patients and the 160 kDa desmoglein-1 in 1 PV patient; 190 kDa periplakin was unexpectedly detected in 8 patients.Conclusion: Indian pemphigus patients showed several unique characteristics, including younger population, predominance of PV, low ELISA indices, lower sensitivity of indirect IF and immunoblotting, and the presence of the 190 kDa periplakin in nearly onethird of patients.Contexte: Le pemphigus pré sente des caracté ristiques diffé rentes selon les ré gions. Objectif: L'é tude visait à dé terminer les caracté ristiques cliniques et immunologiques du pemphigus chez des patients indiens, avant et aprè s le traitement. Mé thode: Divers facteurs, soit l'â ge, le sexe, les manifestations cliniques, le traitement, et la ré action au traitement ainsi que les ré sultats de l'examen histopathologique, de l'immunofluorescence (IF) directe et indirecte, du titrage immunoenzymatique (mé thode ELISA), et de l'immunobuvardage, ont fait l'objet d'analyse chez 26 patients indiens atteints de pemphigus. Ré sultats: Ont é té relevé s 22 cas de pemphigus vulgaire (PV) et 4 cas de pemphigus foliacé (PF). L'IF directe et indirecte s'est ré vé lé e positive chez 95.8% et 56% des patients, respectivement. Les titrages selon la mé thode ELISA é taient faibles dans cette é tude.Quant à l'immunobuvardage, il a permis de dé tecter la pré sence de desmoglé ine-3 de 130 kDa chez 10 patients atteints de PV et de desmoglé ine-1 de 160 kDa chez 1 patient atteint de PV, mais, dé couverte surprenante, la pré sence de pé riplakine de 190 kDa a é té dé celé e chez 8 patients.Conclusions: Plusieurs caracté ristiques particuliè res ont é té relevé es chez les Indiens atteints de pemphigus, notamment l'â ge peu avancé , la pré dominance du PV, de faibles titrages selon la mé thode ELISA, une faible sensibilité de l'IF indirecte et de l'immunobuvardage, et la pré sence de pé riplakine de 190 kDa chez presque un tiers des patients.

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Section: Discussionmentioning

confidence: 97%

Clinical and Immunologic Characterization in 26 Indian Pemphigus Patients

et al. 2013

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“…IgG and IgA antibodies to the C‐terminal domain of BP180 are diagnostic criteria for anti‐BP180‐type mucous membrane pemphigoid, while IgA reactivity with LAD‐1 is a hallmark for the diagnosis of lamina lucida‐type linear IgA bullous dermatosis . However, these antibodies can also be detected in BP . Therefore, the pathogenic significance of these antibodies to the C‐terminal domain of BP180 in PG is currently unknown.…”

Section: Discussionmentioning

confidence: 99%

Clinical and immunological profiles of 25 patients with pemphigoid gestationis

et al. 2014

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“…Ab diversification to epitopes of BP180 and BP230 [108,130] Clinical study Intramolecular and intermolecular Ab spread from collagen XVII to collagen VII [109] Clinical report Intermolecular Bullous pemphigoid develops after pemphigus foliaceus [13,14] Clinical report Intermolecular Cardiomyopathy…”

Section: Diseasementioning

confidence: 98%