Demonstration of Hemoglobin-Reactive Substance in Human Serum

Tuttle, A. H.

doi:10.1126/science.121.3150.701

Cited by 42 publications

(4 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our finding that the plasma of one-half of all normal newborn babies contains haptoglobin differs from that of others who found none (29,28) or who detected haptoglobins in less than 15% of infants at birth (11,20,26,25,35). Most of these workers used starch-gel electrophoresis which, in our experience and in that of others (13) constitutes a less sensitive means of detection.…”

Section: Discussioncontrasting

confidence: 53%

On Haemoglobin Degradation in the Newborn

Watson

Porter

1963

Acta Haematol

View full text Add to dashboard Cite

Section: Discussioncontrasting

confidence: 53%

On Haemoglobin Degradation in the Newborn

Watson

Porter

1963

Acta Haematol

View full text Add to dashboard Cite

“…Tuttle reported that haptoglobins were not present in the blood of newborns and did not appear until after the first few weeks of life (3). Tuttle reported that haptoglobins were not present in the blood of newborns and did not appear until after the first few weeks of life (3).…”

mentioning

confidence: 99%

Development of Serum Haptoglobins in Man.

Woods

Yudowitz

Engle

1961

Experimental Biology and Medicine

View full text Add to dashboard Cite

“…Immunologic Sludies.--To determine the antigenic relationships of the haptoglobins derived from the three serum groups, antisera were produced in rabbits to the electrophoretically isolated haptoglobin hemoglobin complex of each group. The antisera were freed as completely as possible of antibodies to contaminating proteins by repeated absorptions with normal human umbilical cord serum which has been shown to contain inappreciable amounts of haptoglobin (22). Following absorption, these antisera failed to react with umbilical cord serum and free hemoglobin but reacted with normal serum and isolated haptoglobin hemoglobin complexes.…”

Section: Resultsmentioning

confidence: 99%

Comparative Studies on the Physical Characteristics of the Heritable Haptoglobin Groups of Human Serum

Bearn

Franklin

1959

The Journal of Experimental Medicine

View full text Add to dashboard Cite

Ultracentrifugal studies of the α2-proteins of normal human serum before and after the addition of hemoglobin have revealed three separate and clearly distinguishable patterns based on the three major serum haptoglobin groups. Isolation of the three haptoglobin hemoglobin complexes disclosed characteristic patterns for each group. The heterozygote was found to possess electrophoretic and ultracentrifugal components not seen in either homozygote. Separation of normal human serum, to which hemoglobin had been added, by conventional starch zone electrophoresis resulted in three characteristic patterns which also permitted the ready identification of the serum haptoglobin group. The haptoglobin hemoglobin complex from Group 1-1 had the fastest mobility and that of Group 2-2 the slowest. The heterozygote Group 2-1 had an intermediate mobility. Immunologic differences between the haptoglobin hemoglobin complexes of the three groups could not be detected. Some of the variations in the reported electrophoretic and ultracentrifugal patterns of normal human serum could be reasonably ascribed to the group variation of the haptoglobins in man. The physical studies imply that the proteins characteristic of the heterozygote differ in size and charge from those present in either of the two homozygotes and indicate that the genetic control of the synthesis of the serum haptoglobins is probably exceedingly complex.

show abstract

Demonstration of Hemoglobin-Reactive Substance in Human Serum

Cited by 42 publications

References 1 publication

On Haemoglobin Degradation in the Newborn

On Haemoglobin Degradation in the Newborn

Development of Serum Haptoglobins in Man.

Comparative Studies on the Physical Characteristics of the Heritable Haptoglobin Groups of Human Serum

Contact Info

Product

Resources

About