Demonstration of inhibitory factors affecting cell-mediated immunity in patients with Hodgkin's disease

Functional evaluation of T-lymphocytes from peripheral blood and spleens in Hodgkin's disease

“…Both blastogenesis and T-cell colony formation were significantly depressed in HD blood lymphocytes (Table I) as has been previously reported on a larger group of subjects (Moghe et al, 1980;Mukhopadhyaya et al, 1983). However, both blastogenesis and colony formation by spleen lymphocytes were comparable to those shown by blood lymphocytes from normal healthy donors and lymphocytes from 2 normal spleens obtained from accident cases.…”

“…The methods for blastogenesis and colony formation have been described previously (Moghe et al, 1980;Mukhopadhyaya et al, 1983). IL-2 production was studied by assessing the ability of supernatants of 24h lymphocyte cultures stimulated with PHA to support the proliferation of a murine IL-2 dependent T-cell line-CTLL.…”

Impairment of T Lymphocyte Colony Formation in Hodgkin’s Disease: Effect of Soluble Inhibitory Factors on Normal T Lymphocyte Colony Formation Potential

“…In our previous studies we have reported that reduced PHA responsiveness of pa tients with HD could be partially restored after incubating the peripheral blood mononuclear cells (PBMNC) in tissue cul ture (TC) medium. The restoration was better in media containing FCS than in media containing human AB group serum [7]. Further, we have demonstrated that the supernatants of HD lymphocyte cultures supplemented with both human AB serum as well as FCS, and HD sera were inhibi tory for PHA-induced blastogenesis of lymphocytes obtained from normal heal thy donors [7],…”

“…We have therefore carried out further investi gations to study the proliferative potential of circulating T lymphocytes from un treated HD patients in a manner similar to earlier studies [7]. The present report deals with the results of these studies in which we have investigated (i) the ability of T lymphocytes from PBMNC of untreated HD patients to undergo clonal expansion in presence of mitogen PHA in comparis on with that of the normal healthy donors;…”

Natural killer and lymphokine activated killer cell functions in Hodgkin's disease

“…The possible mechanisms of impairment of CMI associated with active HD include an intrinsic defect of T cells, serum inhibitory factors and activation of suppressor monocytes or lymphocytes. We have investigated most of these aspects of T cell deficiencies and their regulation (Gulwani et al, 1986;Karande et al, 1982;Moghe et al, 1980Moghe et al, , 1981Mukhopadhyaya et al, 1983Mukhopadhyaya et al, , 1987a and also the Interleukin-2 (IL-2) mediated events in HD (Damle et al, 1990;Gangal et al, 1987;Joshi et al, 1987;Mukhopadhyaya et al, 1987b).…”