Demonstration of Interference between Dengue Virus Types in Cultured Mosquito Cells using Monoclonal Antibody Probes

Abstract

SUMMARYCultured Aedes albopictus cells (clone C6/36), persistently infected (PI) with dengue virus type 1 (dengue-1) were found resistant to superinfection with dengue virus type 3 (dengue-3). This was determined by indirect immunofluorescent (IF) staining of cultures using monoclonal antibody against a dengue-3 type-specific antigen. Dengue-1 PI cultures stained with this antibody 3 days after superinfection with dengue-3 virus (m.o.i. of 2) had dengue-3 antigen in 0.1 to 1.0% of the cells. Control cultures infected with dengue-3 at the same multiplicity contained denguek3 antigen in greater than 90 % of the cells. The resistance to superinfection was not interferon-mediated, and occurred within 20 h after primary infection. In cultures simultaneously infected with two dengue virus types, one virus type was excluded from replication in most cells. A small population of cells was also found (about 1%) that contained type-specific antigen of both dengue virus types.

Analysis of Reassortment and Superinfection during Mixed Infection of Vero Cells with Bluetongue Virus Serotypes 10 and 17

“…Supcrinfection exclusion (or interference) has bccn noted at the cellular level for many viruses. For example, vertebrate cells infected with Sindbis virus arc refractory to superinfcction with homologous virus (Johnston et al, 1974), and mosquito cells infected with dengue virus arc resistant to supcrinfection with homologous and heterologous strains of dengue virus (Dittmar et al, 1982). Interference has also been noted for other arboviruses in the case of asynchronous infection of the vector (Beaty et al, 1983;Sundin & Beaty, 1988).…”

Dengue 4 Virus Monoclonal Antibodies Identify Epitopes that Mediate Immune Infection Enhancement of Dengue 2 Viruses

“…Previously reported dengue monoclonal antibodies, produced in secretor cells, include antibodies generated to DEN-3 of Dittmar andcolleagues (1980, 1982), which are not widely available, and DE N-2 serotype-reactive and cross-reactive antibodies made at the Walter Reed Army Institute of Research, which have been more completely characterized, distributed to investigators, and used for research and diagnosis Gentry et al, 1982;Gubler et al, 1984;Henchal et al, 1982Henchal et al, , 1983Henchal et al, , 1985. This is the first study of antibody-dependent enhancement of DEN-2 virus infection using monoclonal antibodies raised against a dengue virus of another serotype (DEN-4).…”

Microevolution and virulence of dengue viruses

“…Such criteria include obtaining a virus isolate whose purity is confirmed by plaquing, probe hybridization, and/or neutralization with serotype-specific antisera, ruling out mixed templates, and direct sequencing of multiple ampli-cons from several RNA template preparations, as has been done with polioviruses (Cuervo et al, 2001;Liu et al, 2000). Although the possibility of recombination among dengue viruses may not be remote because dual (serotype) infection of humans has been demonstrated (Gubler et al, 1985;Laille et al, 1991;Lorono-Pino et al, 1999), the probability of simultaneous infection of cells in human or vector hosts may be low because of replication interference (Dittmar et al, 1982). However, this phenomenon has not kept other single-stranded, nonsegmcntcd RNA viruses, such as poliovirus, from often producing inter-and intraserotype recombinants, which has occurred in vaccine (live-attenuated, trivalent Sabin strain) recipients.…”