Demonstration of monolignol β-glucosidase activity of rice Os4BGlu14, Os4BGlu16 and Os4BGlu18 in Arabidopsis thaliana bglu45 mutant

Baiya, Supaporn; Mahong, Bancha; Lee, Sang‐Kyu; Jeon, Jong‐Seong; Cairns, James R. Ketudat

doi:10.1016/j.plaphy.2018.03.026

Cited by 19 publications

(26 citation statements)

References 42 publications

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“…As early as 1995, it was found that P. contorta β-glucosidase activity was located in the cell wall of secondary xylem tissue [14]. AtBGLU45 and AtBGLU46 of Arabidopsis thaliana were also located in the cell wall [16].The rice Os4BGlu16 tagged with a C-terminal GFP was transiently expressed in tobacco leaf epithelial cells and found that the GFP signal was exclusively localized to the plasma membrane and extracellular space in both intact and plasmolysed cells, consistent with an apoplastic or cell wall localization [18]. In this study, in order to explore the PbBGLU1, PbBGLU15, PbBGLU16 subcellular localization, PbBGLU-GFP expression vectors were constructed and transformed into N. tabacum.…”

Section: Subcellular Localization Of Pbbglu1 Pbbglu15 and Pbbglu16mentioning

confidence: 83%

“…Previous studies had shown that the role of pear monolignol glucosides were actually necessary for transmembrane transport [19,21], the monolignol glucosides form can increase the solubility and stability of monolignols, which is very bene cial for the transportation of lignin, then to speci c locations at the cell wall, lignin begins to deglycosylate and polymerize [29]. Many previous studies had reported that genes were localized to the cell wall and extracellular [14,16,18]. In this study we found that green uorescence signals from the expressed fusion PbBGLU1-GFP, PbBGLU15-GFP and PbBGLU16-GFP genes were speci cally distributed in the cell wall, consistent with previous research.…”

Section: Discussionmentioning

confidence: 99%

“…At1g61810 (AtBGLU45), At1g61820 (AtBGLU46) and At4g21760 (AtBGLU47) in Arabidopsis play an important role in the synthesis of Arabidopsis lignin [16,17]. Os4BGLU14, Os4BGLU16 and Os4BGLU18 found in the rice β-glucosidase family have been proved to be functional genes in rice lignin synthesis [18].In addition, a β-glucosidase was identi ed from the poplar tree and named 'PtrBGLU6', which plays an important role in poplar ligni cation and cell wall formation [19].…”

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Comparative Analysis of the β-glucosidase Family in Five Rosaceae Species and their Potential Role on Lignification of Stone Cells in Chinese White Pear

Wang

Yang

Han

et al. 2020

Preprint

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Background: The β-glucosidase BGLU in the glycoside hydrolase family 1 (GH1) is involved in the sugar metabolism of the plant and plays an important role in maintaining the normal physiological function of the plant. Recent studies had shown that β-glucosidase was involved in plant lignification. The lignification in pear fruit is closely related to the formation of pear stone cells, but the BGLU genes family has not been identified in pears.Result: A total of 343 BGLU genes were identified from five species of Rosaceae (Pyrus bretschneideri, Prunus mume, Malus domestica, Prunus avium, Fragaria vesca). According to phylogenetic analysis, 50 PbBGLUs were divided into 8 groups. 298 syntenic pairs were found in intra- and inter-species collinear analysis of five Rosaceae species, found that pears and apples had more syntenic pairs than pear and the other three Rosaceae species. The Ka/Ks analysis of duplication PbBGLU genes in pear indicated that the main mode of expansion of the PbBGLUs was segmental replication and was mainly affected by purification. qRT-PCR showed that the three gene expression patterns of PbBGLU1, PbBGLU15 and PbBGLU16 were basically consistent with the change trend of pear fruit lignin and stone cell content, and may be involved in lignification and stone cell development of pear fruit. Subcellular localization showed that these three candidate genes were all located on the cell wall.Conclusion: In this study, a genome-wide analysis of BGLU genes in five Rosaceae species was carried out, and three candidate genes related to lignification and stone cell development of pear fruits were identified, which laid the foundation for a deeper understanding of the function of BGLU genes in pear fruits and potential in changing pear fruit quality.

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Section: Subcellular Localization Of Pbbglu1 Pbbglu15 and Pbbglu16mentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Comparative Analysis of the β-glucosidase Family in Five Rosaceae Species and their Potential Role on Lignification of Stone Cells in Chinese White Pear

Wang

Yang

Han

et al. 2020

Preprint

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“…It is well-known that monolignols are synthesized from the phenylalanine pathway and either directly secreted to the cell wall or glucosylated by uridine diphosphate glucosyltransferase to generate monolignol glucosides, including p -coumaryl alcohol glucoside, coniferin, and syringin [3–6]. These monolignol glucosides are mostly found in the lignifying secondary wall of gymnosperms and are expected to be storage forms of monolignols, although they do not seem to be required for lignin synthesis [4, 7–12].…”

Section: Introductionmentioning

confidence: 99%

“…Rice Os4BGlu18 is a monolignol β-glucosidase which exhibited high substrate specificity for the monolignol glucosides, coniferin ( k cat / K m >, 31.9 mM −1 s −1 ), syringin ( k cat / K m , 24.0 mM −1 s −1 ), and p -coumaryl alcohol glucoside ( k cat / K m , 1.4 mM −1 s −1 ) [14]. Moreover, Arabidopsis thaliana bglu45 mutant rescued by expression of Os4BGlu18 displayed lower levels for all three substrates when compared with non-rescued line [12]. Since there is no published structure for a monolignol β-glucosidase, the aim of this work was to determine the structure of Os4BGlu18 monolignol β-glucosidase and investigate its interactions with monolignol glucosides via computational docking.…”

Section: Introductionmentioning

confidence: 99%

Structural analysis of rice Os4BGlu18 monolignol β-glucosidase

Baiya

Pengthaisong

Cairns

2020

Preprint

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Monolignol glucosides are storage forms of monolignols, which are polymerized to lignin to strengthen plant cell walls. The conversion of monolignol glucosides to monolignols is catalyzed by monolignol β-glucosidases. Rice Os4BGlu18 β-glucosidase catalyzes hydrolysis of the monolignol glucosides, coniferin, syringin, and p -coumaryl alcohol glucoside more efficiently than other natural substrates. To understand more clearly the basis for substrate specificity of a monolignol β-glucosidase, the structure of Os4BGlu18 was determined by X-ray crystallography. Crystals of Os4BGlu18 and its complex with δ-gluconolactone diffracted to 1.7 and 2.1 Å resolution, respectively. Two protein molecules were found in the asymmetric unit of the P 2 1 2 1 2 1 space group of their isomorphous crystals. The Os4BGlu18 structure exhibited the typical (β/α) 8 TIM barrel of glycoside hydrolase family 1 (GH1), but the four variable loops and two disulfide bonds appeared significantly different from other known structures of GH1 β-glucosidases. Molecular docking studies of the Os4BGlu18 structure with monolignol substrate ligands placed the glycone in a similar position to the δ-gluconolactone in the complex structure and revealed the interactions between protein and ligands. Molecular docking, multiple sequence alignment, and homology modeling identified amino acid residues at the aglycone-binding site involved in substrate specificity for monolignol β-glucosides. Thus, the structural basis of substrate recognition and hydrolysis by monolignol β-glucosidases was elucidated.

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Specific roles of Os4BGlu10, Os6BGlu24, and Os9BGlu33 in seed germination, root elongation, and drought tolerance in rice

Ren

Dong

ChunYan

et al. 2019

Planta

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Demonstration of monolignol β-glucosidase activity of rice Os4BGlu14, Os4BGlu16 and Os4BGlu18 in Arabidopsis thaliana bglu45 mutant

Cited by 19 publications

References 42 publications

Genome-Wide Comparative Analysis of the β-glucosidase Family in Five Rosaceae Species and their Potential Role on Lignification of Stone Cells in Chinese White Pear

Genome-Wide Comparative Analysis of the β-glucosidase Family in Five Rosaceae Species and their Potential Role on Lignification of Stone Cells in Chinese White Pear

Structural analysis of rice Os4BGlu18 monolignol β-glucosidase

Specific roles of Os4BGlu10, Os6BGlu24, and Os9BGlu33 in seed germination, root elongation, and drought tolerance in rice

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