Demonstration of the cryoprotective properties of the fucose-containing polysaccharide FucoPol

Guerreiro, Bruno M.; Freitas, Filomena; Lima, João Carlos; Silva, Jorge; Dionı́sio, Madalena; Reis, Maria A.M.

doi:10.1016/j.carbpol.2020.116500

Cited by 38 publications

(74 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The extrapolations of reducing power to 0.25% FucoPol and its previously observed cryoprotective and photoprotective effects at this concentration [19,21] all supported the postulation of in vitro antioxidant performance. To substantiate the claim that reducing power can be translated to cell protection from oxidative damage, an oxidative damage induction procedure was performed in the presence of 0.25% FucoPol.…”

Section: Fucopol In Vitro Scavenging Potential Of H 2 O 2 -Derived Speciessupporting

confidence: 76%

“…The same logic applies for Trolox. Because chain entanglement or steric hindrance effects are unlikely at this concentration range [39], a plausible explanation for reduced absorbance values might also be the proportion between the reactive polar groups and the structural neutral sugars in the polymer chain Previous work on this polysaccharide showed that outstanding cryoprotective and photoprotective potential could be observed with an optimal 0.25% FucoPol concentration [19,21]. Thus, we extrapolated the antioxidant potential of FucoPol at this concentration by using the FRAP-based modeling previously obtained.…”

Section: Frap Assaysmentioning

confidence: 99%

“…Previous work has shown the multidisciplinary outreach of FucoPol applications. First, it acts as a crystallization inhibitor, successfully cryopreserving several animal cell lines [19,20]. Second, it has a rheological shear-thinning behavior [22] and emulsifying properties [18,23], which are highly desirable in the food industry.…”

Section: Introductionmentioning

confidence: 99%

“…FucoPol is a high-molecular-weight (1.7–5.8 × 10 6 Da) fucose-containing polysaccharide secreted by the Gram-negative bacterium Enterobacter A47 (DSM 23139) [ 17 , 18 ], whose cryoprotective [ 19 , 20 ] and photoprotective [ 21 ] properties were recently demonstrated. FucoPol has a fucose, galactose, glucose, and glucuronic acid hexamer motif (2.0:1.9:0.9:0.5 M ratio), a main chain composed of a →4)-α- l -Fuc p -(1→4)-α- l -Fuc p -(1→3)-β- d -Glc p (1→ trimer repeating unit, and a trimer branch α- d -4,6-pyruvyl-Gal p -(1→4)-β- d -GlcA p -(1→3)-α- d -Gal p (1→ in the C-3 of the first fucose [ 19 ] ( Figure S1 ). FucoPol also contains 13–14 wt% pyruvyl, 3–5 wt% acetyl, and 2–3 wt% succinyl in its acyl composition [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Antioxidant Potential of the Bio-Based Fucose-Rich Polysaccharide FucoPol Supports Its Use in Oxidative Stress-Inducing Systems

et al. 2021

Self Cite

View full text Add to dashboard Cite

Reactive oxygen species (ROS) are dangerous sources of macromolecular damage. While most derive from mitochondrial oxidative phosphorylation, their production can be triggered by exogenous stresses, surpassing the extinction capacity of intrinsic antioxidant defense systems of cells. Here, we report the antioxidant activity of FucoPol, a fucose-rich polyanionic polysaccharide produced by Enterobacter A47, containing ca. 17 wt% of negatively charged residues in its structure. Ferric reducing antioxidant power (FRAP) assays coupled to Hill binding kinetics fitting have shown FucoPol can neutralize ferricyanide and Fe3+-TPTZ species at an EC50 of 896 and 602 µg/mL, respectively, with positive binding cooperativity (2.52 ≤ H ≤ 4.85). This reducing power is greater than most polysaccharides reported. Moreover, an optimal 0.25% w/v FucoPol concentration shown previously to be cryo- and photoprotective was also demonstrated to protect Vero cells against H2O2-induced acute exposure not only by attenuating metabolic viability decay, but also by accentuating post-stress proliferation capacity, whilst preserving cell morphology. These results on antioxidant activity provide evidence for the biopolymer’s ability to prevent positive feedback cascades of the radical-producing Fenton reaction. Ultimately, FucoPol provides a biotechnological alternative for implementation in cryopreservation, food supplementation, and photoprotective sunscreen formula design, as all fields benefit from an antioxidant functionality.

show abstract

Section: Fucopol In Vitro Scavenging Potential Of H 2 O 2 -Derived Speciessupporting

confidence: 76%

Section: Frap Assaysmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Antioxidant Potential of the Bio-Based Fucose-Rich Polysaccharide FucoPol Supports Its Use in Oxidative Stress-Inducing Systems

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…The branches, present at position 3 of the first fucose, are composed of an α-D-4,6-pyruvyl-Galp-(1→4)-β-DGlcAp-(1→3)-α-D-Galp(1→trimer, with two pyruvate caps in the terminal galactose, specifically at position C-4 and C-6 [27,28]. FucoPol was employed for the preparation of a bioactive silver nanocomposite [27] and as a coating agent for iron oxide magnetic nanoparticles (MNP).…”

Section: Introductionmentioning

confidence: 99%

Biosorption of Heavy Metals by the Bacterial Exopolysaccharide FucoPol

2020

Self Cite

View full text Add to dashboard Cite

Despite the efforts for minimizing the usage of heavy metals, anthropogenic activities still generate high amounts of wastewater containing these contaminants that cause significant health and environmental problems. Given the drawbacks of the conventional physical and chemical methods currently used, natural biosorbents (microbial cells or their products) arise as promising environmentally friendly alternatives. In this study, the binding efficiency of the polysaccharide secreted by Enterobacter A47, FucoPol, towards lead (Pb2+), cobalt (Co2+), copper (Cu2+) and zinc (Zn2+) cations was demonstrated. FucoPol revealed a higher performance for the biosorption of Pb2+, with a maximum overall metal removal of 93.9 ± 5.3% and a specific metal uptake of 41.1 ± 2.3 mg/gEPS, from a Pb2+ solution with an initial concentration of 10 mg/L, by a 5 g/L FucoPol solution. The overall metal removal decreased considerably (≤31.3 ± 1.6%) for higher Pb2+ concentrations (48 and 100 mg/L) probably due to the saturation of FucoPol’s binding sites. Pb2+ removal was also less efficient (66.0 ± 8.2%) when a higher FucoPol concentration (10 g/L) was tested. Pb2+ removal efficiency of FucoPol was maximized at pH 4.3, however, it was affected by lower pH values (2.5–3.3). Moreover, the FucoPol’s sorption performance was unaffected (overall metal removal: 91.6–93.9%) in the temperature range of 5–40 °C. These findings demonstrate FucoPol’s great potential for utilization as a biodegradable and safe biosorbent for treating waters and wastewaters contaminated with Pb2+.

show abstract

Cost-Benefit Analysis and Industrial Potential of Exopolysaccharides

Fukuda

Kono

2021

Microbial Exopolysaccharides as Novel and Significant Biomaterials

View full text Add to dashboard Cite

Demonstration of the cryoprotective properties of the fucose-containing polysaccharide FucoPol

Cited by 38 publications

References 60 publications

Antioxidant Potential of the Bio-Based Fucose-Rich Polysaccharide FucoPol Supports Its Use in Oxidative Stress-Inducing Systems

Antioxidant Potential of the Bio-Based Fucose-Rich Polysaccharide FucoPol Supports Its Use in Oxidative Stress-Inducing Systems

Biosorption of Heavy Metals by the Bacterial Exopolysaccharide FucoPol

Cost-Benefit Analysis and Industrial Potential of Exopolysaccharides

Contact Info

Product

Resources

About