Demonstration of the enhancement of gate bias and ionic strength in electric-double-layer field-effect-transistor biosensors

Wu, Chang Run; Wang, Shin Li; Chen, Po Hsuan; Wang, Yu Lin; Wang, Yu Rong; Chen, Jung-Chih

doi:10.1016/j.snb.2021.129567

Cited by 13 publications

(5 citation statements)

References 39 publications

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“…To diagnosis the COVID-19 rapidly and accurately, a few approaches were developed for detection of the RNA [ 9 , 10 ], Protein [ [11] , [12] , [13] ] and virus particles [ 14 , 15 ], including CRISPR-systems [ 16 , 17 ], surface enhanced Raman spectroscopy [ [18] , [19] , [20] ], microfluidic integrated biochip [ 21 , 22 ], electrochemical biosensors [ 23 , 24 ], and field-effect transistor (FET) based biosensors [ 25 ]. Among the existing diagnostic methods, the FET based biosensor delivers several obvious advantages for virus detection, including high selectivity through modified with target receptors, high sensitivity with label free process, real time electrical signal in-situ amplification and recording, cost-effective mass production with microelectronics manufacturing processes, and small size for portable point-of-care test [ [26] , [27] , [28] ]. Graphene, a one atom-thick large area 2D carbon material, has excellent chemical and physical properties in biosensing, such as good biocompatibility, strong interaction with biomolecules through π-π stacking and high intrinsic carrier mobility [ [29] , [30] , [31] ].…”

Section: Introductionmentioning

confidence: 99%

Graphene oxide-graphene Van der Waals heterostructure transistor biosensor for SARS-CoV-2 protein detection

Gao

Chu

Han

et al. 2022

Talanta

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The current outbreaking of the coronavirus SARS-CoV-2 pandemic threatens global health and has caused serious concern. Currently there is no specific drug against SARS-CoV-2, therefore, a fast and accurate diagnosis method is an urgent need for the diagnosis, timely treatment and infection control of COVID-19 pandemic. In this work, we developed a field effect transistor (FET) biosensor based on graphene oxide-graphene (GO/Gr) van der Waals heterostructure for selective and ultrasensitive SARS-CoV-2 proteins detection. The GO/Gr van der Waals heterostructure was in-situ formed in the microfluidic channel through π-π stacking. The developed biosensor is capable of SARS-CoV-2 proteins detection within 20 min in the large dynamic range from 10 fg/mL to 100 pg/mL with the limit of detection of as low as ∼8 fg/mL, which shows ∼3 × sensitivity enhancement compared with Gr-FET biosensor. The performance enhancement mechanism was studied based on the transistor-based biosensing theory and experimental results, which is mainly attributed to the enhanced SARS-CoV-2 capture antibody immobilization density due to the introduction of the GO layer on the graphene surface. The spiked SARS-CoV-2 protein samples in throat swab buffer solution were tested to confirm the practical application of the biosensor for SARS-CoV-2 proteins detection. The results indicated that the developed GO/Gr van der Waals heterostructure FET biosensor has strong selectivity and high sensitivity, providing a potential method for SARS-CoV-2 fast and accurate detection.

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Section: Introductionmentioning

confidence: 99%

Graphene oxide-graphene Van der Waals heterostructure transistor biosensor for SARS-CoV-2 protein detection

Gao

Chu

Han

et al. 2022

Talanta

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“…This extended gate is coupled to another electrode, named gate, by means of the electrolyte. In the case of a nonpolarizable gate electrode, the biolayer is deposited on the extended gate, whereas in the case of a polarizable gate electrode, either the gate or the extended gate could be biofunctionalized. , Here we focus on the most common case of biofunctionalized floating-gate FETs, but the analysis and the design considerations can be easily extended. The equivalent circuit of a biofunctionalized extended-gate FET is displayed in Figure H.…”

Section: Ultrasensitive Bioelectronic Devices: Structures and Materialsmentioning

confidence: 99%

Large-Area Interfaces for Single-Molecule Label-free Bioelectronic Detection

et al. 2022

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Bioelectronic transducing surfaces that are nanometric in size have been the main route to detect single molecules. Though enabling the study of rarer events, such methodologies are not suited to assay at concentrations below the nanomolar level. Bioelectronic field-effect-transistors with a wide (μm 2 −mm 2 ) transducing interface are also assumed to be not suited, because the molecule to be detected is orders of magnitude smaller than the transducing surface. Indeed, it is like seeing changes on the surface of a one-kilometer-wide pond when a droplet of water falls on it. However, it is a fact that a number of large-area transistors have been shown to detect at a limit of detection lower than femtomolar; they are also fast and hence innately suitable for point-of-care applications. This review critically discusses key elements, such as sensing materials, FET-structures, and target molecules that can be selectively assayed. The amplification effects enabling extremely sensitive large-area bioelectronic sensing are also addressed.

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“…Hence, the most common method to rectify this issue is by significantly reducing the ionic strength of solutions by desalting or diluting the solutions to overcome the Debye screening effect [ 173 ]. This, however, requires further complicated sample pre-treatments [ 174 ] and may change biomolecule composition, resulting in the loss of target analyte activity and binding affinity [ 175 ]. Due to this issue, the true capability for detection of all FET-based biosensors, including HEMT, is limited since the direct detection of clinical samples is unfeasible [ 176 ].…”

Section: Challenges and Opportunities Of Heterojunction-based Hemtmentioning

confidence: 99%

Status and Prospects of Heterojunction-Based HEMT for Next-Generation Biosensors

et al. 2023

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High electron mobility transistor (HEMT) biosensors hold great potential for realizing label-free, real-time, and direct detection. Owing to their unique properties of two-dimensional electron gas (2DEG), HEMT biosensors have the ability to amplify current changes pertinent to potential changes with the introduction of any biomolecules, making them highly surface charge sensitive. This review discusses the recent advances in the use of AlGaN/GaN and AlGaAs/GaAs HEMT as biosensors in the context of different gate architectures. We describe the fundamental mechanisms underlying their operational functions, giving insight into crucial experiments as well as the necessary analysis and validation of data. Surface functionalization and biorecognition integrated into the HEMT gate structures, including self-assembly strategies, are also presented in this review, with relevant and promising applications discussed for ultra-sensitive biosensors. Obstacles and opportunities for possible optimization are also surveyed. Conclusively, future prospects for further development and applications are discussed. This review is instructive for researchers who are new to this field as well as being informative for those who work in related fields.

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Demonstration of the enhancement of gate bias and ionic strength in electric-double-layer field-effect-transistor biosensors

Cited by 13 publications

References 39 publications

Graphene oxide-graphene Van der Waals heterostructure transistor biosensor for SARS-CoV-2 protein detection

Graphene oxide-graphene Van der Waals heterostructure transistor biosensor for SARS-CoV-2 protein detection

Large-Area Interfaces for Single-Molecule Label-free Bioelectronic Detection

Status and Prospects of Heterojunction-Based HEMT for Next-Generation Biosensors

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