Demonstration of the simultaneous presence of transferrin, hemopexin and albumin in the same adult rat hepatocyte

Foucrier, Jean; Kraemer, Michel; Vassy, Jany; Chalumeau, M.T.

doi:10.1016/0045-6039(79)90016-2

Cited by 20 publications

(4 citation statements)

References 22 publications

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“…The rabbit antisera against pure rat transferrin, a,-antitrypsin, a-fetoprotein, albumin and angiotensinogen have been described elsewhere [l, 3,8,19,341. For immunochemical staining, these were used at dilutions of 1:40 to 1:lOO.…”

Section: Antiseramentioning

confidence: 99%

“…according to Takahara et al [47] and Carlson and Stenflo [8], who have shown that this protein accounts for 1/26 of the total of rat serum proteins. Pure albumin and pure angiotensinogen were prepared as previously described [4, 391. Pure transferrin [19] and fibrinogen purified according to the method of Capet-Antonini [7] were kindly provided by Dr J. Foucrier and C. Legrzle, respectively. When necessary, samples to be tested were first concentrated up to 150 times by ultrafiltration through CF-5OA Centriflo membranes (Amicon).…”

Section: Plasma-protein Assaysmentioning

confidence: 99%

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Plasma-protein production by rat hepatoma cells in culture, their variants and revertants

et al. 1986

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Section: Antiseramentioning

confidence: 99%

Section: Plasma-protein Assaysmentioning

confidence: 99%

Plasma-protein production by rat hepatoma cells in culture, their variants and revertants

et al. 1986

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“…Preparation of rabbit anti-rat transferrin antibodies Procedures for isolating rat transferrin and obtaining the corresponding antiserum have been described elsewhere (Foucrier et al, 1979). The specificity of the antiserum was assessed both by immunoelectrophoresis and by crossed immunoelectrophoresis (Weeke, 1973).…”

Section: Liver Cell Preparationmentioning

confidence: 99%

Correlation between plasma membrane surface area and transferrin secretion rate in isolated hepatocytes

Pechinot

Foucrier

Feldmann

1986

Journal Cellular Physiology

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It is generally considered that in exocytosis the size of the secreting cells does not increase when the membranes of exocytosis vesicles fuse with the plasma membrane. As the factors involved in the regulation of this phenomenon are poorly understood, we thought it worthwhile to investigate the relationship between the plasma membrane surface area and secretory activity. Isolated rat hepatocytes were prepared by liver collagenase perfusion. Secretion of the plasma protein, transferrin (Tf) was detected at the single cell level with specific anti-rat transferrin antibodies using the reverse hemolytic plaque test. Hepatocyte surface and hemolytic ring surface areas were calculated from diameters of hepatocyte and hemolytic plaque measured after 5h of incubation. A highly significant correlation was established between the plaque-forming hepatocyte surface areas and the corresponding hemolytic surface areas. This result was confirmed using an automatic image analysis method. Two-month-old rats were compared to 4-month-old rats. We observed that the ratio of the quantity of transferrin secreted by hepatocytes to the hepatocyte surface area was constant for a given incubation time, whatever the size of the hepatocytes. These results suggest that the plasma membrane surface area of hepatocytes may constitute a limiting factor in Tf secretion.

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“…In these studies, we noticed that the reaction product specific for albumin antigenicity was in close association with the cytosolic surface of bound ribosomes. Evidence for the presence of other nascent plasma proteins on attached hepatic ribosomes has previously been published: albumin (Feldman et al, 1972;Guillouzo et al, 1976), transferrin and hemopexin (Foucrier et al, 1979), and fibrinogen . That the cytosolic surface of ribosomes is a site where nascent proteins could acquire their antigenic determinants does not agree with the present conception of protein synthesis conveyed by the signal hypothesis (Blobel, 1977;Palade, 1975).…”

mentioning

confidence: 98%

Ultrastructural immunocytochemistry of nascent albumin topology: Proposed cytosolic folding and membrane transit of the protein

LeBouton

Masse

1981

Anat. Rec.

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The relationship of nascent albumin and hepatocyte organelles was studied with the immunoperoxidase reaction in rats given various drugs to alter cellular albumin content. colchicine was used to increase intracellular albumin. Cycloheximide inhibited synthesis but allowed nascent albumin to remain with its ribosome of origin. Puromycin also inhibited synthesis but released albumin from its ribosome. There was no difference in the appearance of attached ribosomes in hepatocytes from saline-injected rats and those given colchicine or cycloheximide. In these cases, membranes of the endoplasmic reticulum were consistently decorated with ribosomes positive for the presence of albumin antigenicity on their cytosolic surface. The cisternal and cytosolic compartments were negative. The situation after puromycin was different. Here the membranes appeared to be denuded of ribosomes and reaction product, indicative of albumin, was present only on the lumenal surface. To determine whether puromycin had caused the release of ribosomes, sections from puromycin-treated cells were stained nonspecifically with uranyl acetate. This showed that the normal amount of ribosomes was still bound but that they could not be seen when a probe specific only for albumin was used. It appears that nascent albumin can associate with its ribosome within the cytosol. Also, apparently after albumin passes through the membrane of the rough endoplasmic reticulum, it remains attached to its lumenal surface. A model incorporating cytosolic folding of albumin followed by its entropic membrane transit is presented.

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Demonstration of the simultaneous presence of transferrin, hemopexin and albumin in the same adult rat hepatocyte

Cited by 20 publications

References 22 publications

Plasma-protein production by rat hepatoma cells in culture, their variants and revertants

Plasma-protein production by rat hepatoma cells in culture, their variants and revertants

Correlation between plasma membrane surface area and transferrin secretion rate in isolated hepatocytes

Ultrastructural immunocytochemistry of nascent albumin topology: Proposed cytosolic folding and membrane transit of the protein

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