Denaturing Gradient Gel Electrophoresis (DGGE) as a tool for the characterisation of Brachionus sp. strains

Dooms, S.; Papakostas, Spiros; Hoffman, Stefan; Delbare, D.; Dierckens, Kristof; Triantafyllidis, Alexander; Wolf, Tania De; Vadstein, Ôlav; Abatzopoulos, Theodore J.; Sorgeloos, Patrick; Bossier, Peter

doi:10.1016/j.aquaculture.2006.10.001

Cited by 21 publications

(15 citation statements)

References 23 publications

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“…This is presently true at least in the B. plicatilis group, for which much knowledge has been previously widely developed, as it remains by far the best studied rotifer species complex from a genetic point of view (e.g. Gó mez, 2005;Dooms et al, 2007).…”

Section: Discussionmentioning

confidence: 97%

Disentangling the morphological stasis in two rotifer species of the Brachionus plicatilis species complex

et al. 2007

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The taxonomic uncertainty surrounding cryptic species complexes has traditionally been resolved using lengthy experimental approaches, while, since the advent of PCR based techniques the number of cryptic species described in a variety of taxa is increasing steadily.Here we formally describe a new rotifer species of the Brachionus plicatilis complex: Brachionus manjavacas n.sp., disentangling what was known as a morphological stasis. Detailed morphological analyses demonstrated significant differences in body shape and size between B. manjavacas and B. plicatilis s.s., analysed by geometric morphometrics; unfortunately these statistical differences are not taxonomically reliable because of wide overlaps. Size and asymmetry of masticatory apparatus, named trophi, observed by SEM, gave similar results, with taxonomic ambiguity. Only the shape of small pieces of the trophi, named satellites, were consistently different between the species. On a strictly classical taxonomical basis it is absolutely useful to name new species on morphological bases, as we did, and to assess their status as distinct entities. Nevertheless, the two species are broadly similar; therefore, we do not suggest using the small differences in shape of satellites of trophi to identify the species for further ecological studies, but to continue discriminating them on genetic marker bases.

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Section: Discussionmentioning

confidence: 97%

Disentangling the morphological stasis in two rotifer species of the Brachionus plicatilis species complex

et al. 2007

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“…Cayman and Brachionus sp. Austria have been identified as common in hatcheries (Papakostas, Dooms, Triantafyllidis, Deloof, Kappas, Dierckens, De Wolf, Bossier, Vadstein, Kui, Sorgeloos & Abatzopoulos 2006; Dooms, Papakostas, Hoffman, Delbare, Dierkens, Triantafyllidis, De Wolf, Vadstein, Abatzopoulos, Sorgeloos & Bossier 2007; Kostopoulou & Vadstein 2007; Baer, Langdon, Mills, Schulz & Hamre 2008). The clarification of the taxonomical situation will continue in the coming years.…”

Section: Rotifersmentioning

confidence: 99%

Live feeds for early stages of fish rearing

Conceição

Yúfera

Makridis

et al. 2010

Aquaculture Research

370

282

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Despite the recent progress in the production of inert diets for fish larvae, feeding of most species of interest for aquaculture still relies on live feeds during the early life stages. Independently of their nutritional value, live feeds are easily detected and captured, due to their swimming movements in the water column, and highly digestible, given their lower nutrient concentration (water content>80%). The present paper reviews the main types of live feeds used in aquaculture, their advantages and pitfalls, with a special emphasis on their nutritional value and the extent to which this can be manipulated. The most commonly used live feeds in aquaculture are rotifers (Brachionus sp.) and brine shrimp (Artemia sp.), due to the existence of standardized cost‐effective protocols for their mass production. However, both rotifers and Artemia have nutritional deficiencies for marine species, particularly in essential n‐3 highly unsaturated fatty acids (HUFA, e.g., docosahexaenoic acid and eicosapentaenoic acid). Enrichment of these live feeds with HUFA‐rich lipid emulsions may lead to an excess dietary lipid and sub‐optimal dietary protein content for fish larvae. In addition, rotifers and Artemia are likely to have sub‐optimal dietary levels of some amino acids, vitamins and minerals, at least for some species. Several species of microalgae are also used in larviculture. These are used as feed for other live feeds, but mostly in the ‘green water’ technique in fish larval rearing, with putative beneficial effects on feeding behaviour, digestive function, nutritional value, water quality and microflora. Copepods and other natural zooplankton organisms have also been used as live feeds, normally with considerably better results in terms of larval survival rates, growth and quality, when compared with rotifers and Artemia. Nonetheless, technical difficulties in mass‐producing these organisms are still a constraint to their routine use. Improvements in inert microdiets will likely lead to a progressive substitution of live feeds. However, complete substitution is probably years away for most species, at least for the first days of feeding.

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“…The presence of B. 'Nevada' has been con-¢rmed in a number of hatcheries around Europe (Papakostas et al 2006;Dooms et al 2007).…”

Section: Experimental Stock Populationmentioning

confidence: 99%

“…‘Nevada’ and B . ‘Austria’, apart from B. plicatilis s.s. (Papakostas, Dooms, Triantafyllidis, Deloof, Kappas, Dierckens, De Wolf, Bossier, Vadstein, Kui, Sorgeloos & Abatzopoulos 2006; Dooms, Papakostas, Hoffman, Delbare, Dierckens, Triantafyllidis, De Wolf, Vadstein, Abatzopoulos, Sorgeloos & Bossier 2007). The latter lineages have been studied sporadically ( B .…”

Section: Introductionmentioning

confidence: 99%

Morphometric changes in a strain of the lineage ‘Nevada’, belonging to theBrachionus plicatilis(Rotifera) complex

Kostopoulou

Miliou

Verriopoulos

2009

Aquaculture Research

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The rotifer Brachionus plicatilis is an important component of aquaculture as a larval feed. Its taxonomic status has been recently re‐defined as a species complex, consisting of at least 14 new species/lineages. This study deals with the lineage Brachionus‘Nevada’, which has been shown to occur in European hatcheries. A strain of B. ‘Nevada’ was mass cultured using two commonly applied feeding regimes and analysed in terms of its morphometry. A new formula was proposed for the calculation of volume, which can be used as an index of adequacy of rotifers as feed for fish larvae. The results were related to life cycle parameters. The pre‐reproductive and reproductive phases were divided into distinct size groups. Differences were also found between the two diets in morphometry and demography. Rotifers of a larger size (yeast‐based diet) showed a lower growth rate and a longer reproductive period, lifespan and mean generation time compared with smaller‐sized rotifers (Culture Selco®‐based diet). In terms of lorica length, the present study's strain of B. ‘Nevada’ (238.5 μm) was intermediate between values reported for Brachionus ibericus (193.5 μm) and B. plicatilis sensu stricto (299 μm).

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Denaturing Gradient Gel Electrophoresis (DGGE) as a tool for the characterisation of Brachionus sp. strains

Cited by 21 publications

References 23 publications

Disentangling the morphological stasis in two rotifer species of the Brachionus plicatilis species complex

Disentangling the morphological stasis in two rotifer species of the Brachionus plicatilis species complex

Live feeds for early stages of fish rearing

Morphometric changes in a strain of the lineage ‘Nevada’, belonging to theBrachionus plicatilis(Rotifera) complex

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