Dendritic Cell Amiloride Sensitive Channels Mediate Sodium-induced Inflammation and Hypertension

Barbaro, Natália R.; Foss, Jason D.; Kryshtal, Dmytro O.; Tsyba, Nikita; Kumaresan, Shivani; Xiao, Liang; Mernaugh, Raymond L.; Itani, Hana A.; Chen, Wei; Dikalov, Sergey; Titze, Jens; Knollmann, Björn C.; Harrison, David G.; Kirabo, Annet

doi:10.2139/ssrn.3151993

Cited by 39 publications

(83 citation statements)

References 49 publications

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“…These authors also reported that isoketal protein adducts were elevated in circulating monocytes and DCs from hypertensive humans. More recently, it was demonstrated in vitro that like Ang II, increased extracellular sodium through amiloride‐sensitive sodium channels can cause ROS generation in DCs, formation of isoketal protein adducts and production of IL‐1β (Barbaro et al ., ). Furthermore, these authors showed that high extracellular sodium‐stimulated DCs could activate T cells isolated from mice in which salt‐sensitive hypertension was induced, but not T cells from untreated mice.…”

Section: Role Of Adaptive Immune System In Hypertensionmentioning

confidence: 97%

Role of immune cells in hypertension

Caillon

Paradis

Schiffrin

2018

British J Pharmacology

130

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Inflammatory processes have been shown to play an important role in the mechanisms involved in the pathogenesis of hypertension. Innate and adaptive immune responses participate in BP elevation and end-organ damage. Here, we discuss recent studies focusing on novel inflammatory and immune mechanisms that play roles in BP elevation. Different subpopulations of cells involved in innate and adaptive immune responses, such as dendritic cells, monocytes/macrophages and NK cells, on the one hand, and B and T lymphocytes, on the other, contribute to the vascular and kidney injury in hypertension. Unconventional innate-like T cells such as γδ T cells also participate in hypertensive mechanisms by priming both innate and adaptive immune cells, contributing to trigger vascular inflammation and BP elevation. These cells exert their effects in part via production of various cytokines including pro-inflammatory IFN-γ and IL-17 and anti-inflammatory IL-10. The present review summarizes some of these immune mechanisms that participate in the pathophysiology of hypertension.

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Section: Role Of Adaptive Immune System In Hypertensionmentioning

confidence: 97%

Role of immune cells in hypertension

Caillon

Paradis

Schiffrin

2018

British J Pharmacology

130

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“…We chose different NaCl concentrations to represent a change in the cellular environment. NaCl is a well-studied stressor in yeast cells and is relevant in human cells in the context of immune cell activation [22][23][24][25][26] and cell death 13 . We have developed two cell culture methodologies for time point (TP) measurements from continuously growing cells ( Figure 2b These changes need to be considered to accurately compute the desired pump profile and failure to do so can result in significant error in the pump profile as plotted in Figure 3.…”

Section: Resultsmentioning

confidence: 99%

Generating kinetic environments to study dynamic cellular processes in single cells

Thiemicke

Jashnsaz

et al. 2019

Preprint

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Cells of any organism are consistently exposed to changes over time in their environment. The kinetics by which these changes occur are critical for the cellular response and fate decision. It is therefore important to control the temporal changes of extracellular stimuli precisely to understand biological mechanisms in a quantitative manner. Most current cell culture and biochemical studies focus on instant changes in the environment and therefore neglect the importance of kinetic environments. To address these shortcomings, we developed two experimental methodologies to precisely control the environment of single cells. These methodologies are compatible with standard biochemistry, molecular, cell and quantitative biology assays. We demonstrate applicability by obtaining time series and time point measurements in both live and fixed cells. We demonstrate the feasibility of the methodology in 2 yeast and mammalian cell culture in combination with widely used assays such as flow cytometry, time-lapse microscopy and single-molecule RNA Fluorescent in-situ Hybridization.Our experimental methodologies are easy to implement in most laboratory settings and allows the study of kinetic environments in a wide range of assays and different cell culture conditions. Recent pioneering studies have provided insights into how molecular processes differ in kinetic environments in comparison to instant changes in the environment 2-5,10-15 . These studies have often relied on two main approaches to deliver the desired kinetic perturbations on the cells. One is the use of specialized, small-scale and custom designed microfluidic setups [16][17][18][19][20] .But fabricating these devices is often complex, may require specialized equipment to produce,

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“…Dendritic cells activated by excess sodium produce increased IL‐1β and promote T cell production of cytokines IL‐17A and IFN‐γ. When adoptively transferred into naive mice, dendritic cells primed with high salt trigger hypertension in response to a subpressor dose of Ang II (Barbaro et al ., ).…”

Section: Introductionmentioning

confidence: 97%

Salt, inflammation, IL‐17 and hypertension

Wenzel

Bode

Kurts³

et al. 2018

British J Pharmacology

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Traditionally, arterial hypertension and subsequent end-organ damage have been attributed to haemodynamic factors, but increasing evidence indicates that inflammation also contributes to the deleterious consequences of this disease. The immune system has evolved to prevent invasion of foreign microorganisms and to promote tissue healing after injury. However, this beneficial activity comes at a cost of collateral damage when the immune system overreacts to internal injury, such as prehypertension. Over the past few years, important findings have revolutionized hypertension research. Firstly, in 2007, a seminal paper showed that adaptive immunity is involved in the pathogenesis of hypertension. Secondly, salt storage in the skin and its consequences for cardiovascular physiology were discovered. Thirdly, after the discovery that salt promotes the differentiation of CD4 T cells into T 17 cells, it was demonstrated that salt directly changes several cells of the innate and adaptive immune system and aggravates autoimmune disease but may improve antimicrobial defence. Herein, we will review pathways of activation of immune cells by salt in hypertension as the framework for understanding the multiple roles of salt and immunity in arterial hypertension and autoimmune disease.

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Dendritic Cell Amiloride Sensitive Channels Mediate Sodium-induced Inflammation and Hypertension

Cited by 39 publications

References 49 publications

Role of immune cells in hypertension

Role of immune cells in hypertension

Generating kinetic environments to study dynamic cellular processes in single cells

Salt, inflammation, IL‐17 and hypertension

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