Dendrobium nobile Lindl polysaccharides improve testicular spermatogenic function in streptozotocin‐induced diabetic rats

Abstract

Dendrobium nobile Lindl polysaccharides (DNLP) exhibited various biological functions. This study aimed to investigate the protective effects of DNLP on testicular spermatogenic function in streptozotocin (STZ)‐induced diabetic rats in comparison with metformin. The blood glucose level was significantly increased and the homeostatic model assessment for insulin resistance (HOMA‐IR) aggravated markedly in diabetic rats. The weight of testis and epididymis, and the sperm number and motility were decreased in the diabetic rats. The pathologic changes occurred in the spermatogenic tubules along with the decreased number of spermatogenic cells, downregulated proliferating cell nuclear antigen (PCNA) and Sirtuin 1 (SIRT1) expression and increased cell apoptosis in the testes. Compared with the model group, DNLP and metformin treatment significantly decreased the level of blood glucose, improved the HOMA‐IR, and increased the weight of testis and epididymis, as well as the sperm number and sperm motility. Furthermore, the pathologic changes in the spermatogenic tubules improved significantly with increased number of spermatogenic cells, the upregulation of PCNA and SIRT1 and suppression of cell apoptosis in the testes. Collectively, our study for the first time examined the effects of DNLP on the male reproductive system of STZ‐induced diabetic rats, and indicated that DNLP was protective against diabetes mellitus‐induced testis injury via increasing the proliferation, inhibiting cell apoptosis and upregulating SIRT1 expression in testicular spermatogenic cells.

Therapeutic Application of Dendrobium fimbriatum Hook for Retinopathy Caused by Ultraviolet Radiation and Chemotherapy Using ARPE-19 Cells and Mouse Retina

“…The most relevant phytochemical elements in dendrobium species include polysaccharides, alkaloids, and polyphenols, and the bioactive components of dendrobium include gigantol, moscatilin, dendrofalconerol A, dendrochrysanene, crispidatin, confusarin, denbinobin, and chrysotobibenzyl [ 26 ]. Polysaccharides are among the main active components of dendrobium, exerting antioxidant, anti-inflammatory, and anti-apoptotic effects [ 27 , 28 , 29 , 30 ]. In this study, we found that the main active compounds of DFE were moscatilin, gigantol, and chrysotobibenzyl ( Figure 2 ).…”

Icariin Ameliorates Spermatogenesis Disorder in Obese Mice Induced by High‐Fat Diet through Regulating the Glycolytic Pathway

“…Previously, we found that Dendrobium nobile-derived polysaccharides (DNP) could increase the activity of LDHA along with the production of lactate and have anti-apoptotic effects on spermatogenic cells, thereby improving testicular spermatogenic function in diabetic patients and animals. [61][62][63] What is more, our team has demonstrated that increased intake of ICA protected diabetic rats from high-fat diet and streptozotocininduced spermatogenesis abnormalities. [23] The present results also confirmed that the expression of apoptosis-related genes was increased, and the mRNA and protein expression level of LDHA, key enzymes related to glycolysis has reduced in obese mice.…”

Bezafibrate alleviates diabetes-induced spermatogenesis dysfunction by inhibiting inflammation and oxidative stress

“…However, there is no evidence about the protective role of bezafibrate on diabetes-induced male spermatogenesis dysfunction. STZ injection-induced DM mice model can mimic the clinical features of patients with type 1 diabetes and has been used to employ diabetes-related male fertility in several previous studies [ [25] , [26] , [27] ]. Thus, this study was designed to investigate the effects of bezafibrate on testicular function using streptozotocin (STZ)-induced type 1 DM mice model and to discover the underlying molecular mechanism.…”