Dengue immunoassay with an LSPR fiber optic sensor

Abstract

Dengue fever is a viral disease that affects millions of people worldwide. Specific tests for dengue are not usually performed due to high costs, complicated procedures and, in some cases, long time to yield a result. For widespread use of specific tests to be possible, fast, reliable and fairly simple methods are needed. In this paper, we present a new dengue diagnostic method for the acute phase of the infection. The method proposed uses an all-optical fiber sensor based on Localized Surface Plasmon Resonance (LSPR) and specular reflection from gold nanoparticles (AuNPs). Dengue anti-NS1 antibody was immobilized on AuNPs deposited on the endface of a standard multimode fiber (62.5 µm/125 µm). The sensor is able to detect NS1 antigen at different concentrations, with limit of quantification estimated to be 0.074 μg/ml = 1.54 nM. These results indicate that the sensor could potentially be used for dengue diagnosis in the acute phase of the infection.

Engineering a Localized Surface Plasmon Resonance Platform for Molecular Biosensing

“…The proposed Open Journal of Applied Sciences nm [19]. Moreover, the refractive index of the adsorbate shell layer should also be appraised.…”

“…LSPR sensors can be explored as fast, reliable, low-cost, and fairly simple tool for medical diagnosis. Various examples of LSPR biosensors were attributed to the diagnosis of relevant medical disease, as Alzheimer [14], preeclampsia [15], influenza [16], HIV-1 [17], hepatitis B infections [18] and Dengue virus [19], as well as for intracellular protein sensing [20].…”

Diagnosing dengue virus infection: rapid tests and the role of micro/nanotechnologies

“…90 Resonance (LSPR) to diagnose dengue using an all-optical fiber sensor exploiting specular reflection from gold nanoparticles. 91 Dengue anti-NS1 antibody was immobilized on AuNPs deposited on the end face of a standard multimode fiber. This sensor detected NS1 antigen down to a limit of quantification of 0·074 μg/ml, which was in the range of serum NS1 antigen concentrations (0.04 to 2 μg/ml) detected by a sensitive capture ELISA in patients during the acute phase of the dengue infection, and presented its potential for early diagnosis of dengue.…”

Microfiber coupler based biosensor incorporating a layer of gold nanoparticles with improved sensitivity

“…Moreover, it has been shown that the biomolecular interactions on the nanoparticle surface can change the evanescent field distribution. For example, the authors in [11] demonstrated that in order to impart selectivity to a fiber-optic end-face sensor based on LSPR, a material with affinity to the desired target-substance can be attached to the nanoparticles. In this work, we investigated the possibility of utilizing the properties of gold nanoparticles coated on the surface of the MFC in order to improve the sensitivity of our recently proposed MFC immunosensor.…”