Denitrification behavior and microbial community spatial distribution inside woodchip-based solid-phase denitrification (W-SPD) bioreactor for nitrate-contaminated water treatment

Zhao, Jiamin; Feng, Chuanping; Tong, Shuang; Dong, Shanshan; Peng, Tong

doi:10.1016/j.biortech.2017.11.011

Cited by 87 publications

(25 citation statements)

References 36 publications

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“…Specifically, nitrate, as the most dominant nitrogen species, is widely distributed in aquatic environments due to the excessive use of nitrogenous fertilizer in agricultural lands and also as a consequence of the improper disposal of untreated industrial wastes . However, excessive nitrate and nitrite (intermediate of nitrate reduction) loadings also lead to severe water deterioration, such as eutrophication, and have toxicological effects on humans once the contaminated water enters drinking water supplies . In consideration of environmental sustainability and human health, the U.S.…”

Section: Introductionmentioning

confidence: 99%

“…1 However, excessive nitrate and nitrite (intermediate of nitrate reduction) loadings also lead to severe water deterioration, such as eutrophication, and have toxicological effects on humans once the contaminated water enters drinking water supplies. 2 In consideration of environmental sustainability and human health, the U.S. Environment Protection Agency (EPA) has set the maximum contaminant levels (MCL) of nitrate and nitrite at 10 and 1.0 mg L −1 (measured as N) in drinking water, respectively.…”

Section: Introductionmentioning

confidence: 99%

“…Environment Protection Agency (EPA) has set the maximum contaminant levels (MCL) of nitrate and nitrite at 10 and 1.0 mg L −1 (measured as N) in drinking water, respectively. 3 Heterotrophic denitrification, with organic carbon sources as electron donors for bacterial metabolic chain, 2,4,5 has been demonstrated as one of the most promising techniques in purifying nitrate-contaminated aquifers due to its simplicity, sustainability and low cost. 6 In this process, nitrate (NO 3 − ) from aqueous solution can be converted to nitrite (NO 2 − ), nitric oxide (NO), nitrous oxide (N 2 O) and eventually N 2 gas escaping into the atmosphere.…”

Section: Introductionmentioning

confidence: 99%

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Insights into simultaneous microbial chromium and nitrate reduction: inhibitory effects and molecular mechanisms

Chen

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND: The potential effect of chromium on the denitrification process has drawn much attention recently. The efficient remediation of groundwater contaminated by both hexavalent chromate [Cr(VI)] and nitrate (NO 3 − ) was hampered due to the deficiency of denitrification in the presence of Cr(VI). The goal of this work was to understand the mechanisms of how Cr(VI) affected denitrification. RESULTS:To better understand the underlying nature, Cr(VI) effects on denitrification were investigated in batch. Negative effects of Cr(VI) exposure on denitrification were confirmed and ascribed to restriction of bacterial activities, by regulating functional genes expression and altering community composition. Stronger inhibition of the expression of denitrifying genes was observed with increased Cr(VI) loading (0-50 mg L −1 ). The critical inhibitory concentration and IC 50 of Cr(VI) on electron transport system activities were calculated to be 3.21 and 4.87 mg L −1 , respectively. Further amplicon analysis revealed that Betaproteobacteria were enriched, implying their potential key role in simultaneously removing nitrate and Cr(VI). The presence of Cr(VI) also upregulated the metabolic activities related to apoptosis. CONCLUSION: These findings shed light on the biogeochemical fates of Cr(VI) and NO 3− in aquifers, and may contribute in enhancing their practical application for bioremediation using microbial processes. ETSA assayElectron transport activity of the bacteria was measured by reducing 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetrazolium wileyonlinelibrary.com/jctb

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Insights into simultaneous microbial chromium and nitrate reduction: inhibitory effects and molecular mechanisms

Chen

et al. 2019

J of Chemical Tech & Biotech

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“…were identified as important bacteria for denitrification at low temperatures (Jang et al., 2019). Denitrification mechanisms present along the height of an up‐flow bench reactor have also been investigated, showing that carbon‐degrading, denitrifying, and fermentative microorganisms were important for bioreactor performance (Zhao et al., 2018). Additionally, the abundance of each type of microorganism was determined to be correlated to resource availability (nitrate, dissolved carbon), which varied along the height of the reactor.…”

Section: Factors Affecting the Microbial Community Within Woodchip Bioreactorsmentioning

confidence: 99%

Denitrifying bioreactor microbiome: Understanding pollution swapping and potential for improved performance

et al. 2021

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Denitrifying woodchip bioreactors are a best management practice to reduce nitrate–nitrogen (NO3–N) loading to surface waters from agricultural subsurface drainage. Their effectiveness has been proven in many studies, although variable results with respect to performance indicators have been observed. This paper serves the purpose of synthesizing the current state of the science in terms of the microbial community, its impact on the consistency of bioreactor performance, and its role in the production of potential harmful by‐products including greenhouse gases, sulfate reduction, and methylmercury. Microbial processes other than denitrification have been observed in these bioreactor systems, including dissimilatory nitrate reduction to ammonia (DNRA) and anaerobic ammonium oxidation (anammox). Specific gene targets for denitrification, DNRA, anammox, and the production of harmful by‐products are identified from bioreactor studies and other environmentally relevant systems for application in bioreactor studies. Lastly, cellulose depletion has been observed over time via increasing ligno‐cellulose indices, therefore, the microbial metabolism of cellulose is an important function for bioreactor performance and management. Future work should draw from the knowledge of soil and wetland ecology to inform the study of bioreactor microbiomes.

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“…The lower C content of the unwashed, in-ditch woodchips (17.4 ± 0.5%; Table 1), which were used in the denitrification potential tests, reflected the relatively low C content of soil particles attached to the woodchip surface. Woodchips from the diversion bioreactor were not Microbial community characteristics can influence denitrification rates in woodchip bioreactors [32,33]. Since a labile carbon supplement was used in the denitrification assays, the low denitrification potential of the diversion bioreactor woodchips was likely caused by differences in the microbial community instead of carbon availability.…”

Section: Microbial and Physiochemical Properties Of Woodchips And Soilmentioning

confidence: 99%

Nitrate Removal and Woodchip Properties across a Paired Denitrifying Bioreactor Treating Centralized Agricultural Ditch Flows

Maxwell

Christianson

Cooke

et al. 2021

Water

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Treatment of nitrate loads by denitrifying bioreactors in centralized drainage ditches that receive subsurface tile drainage may offer a more effective alternative to end-of-pipe bioreactors. A paired denitrifying bioreactor design, consisting of an in-ditch bioreactor (18.3 × 2.1 × 0.2 m) treating ditch base flow and a diversion bioreactor (4.6 × 9.1 × 0.9 m) designed to treat high-flow events, was designed and constructed in an agricultural watershed (3.2 km2 drainage area) in Illinois, USA. Flow and water chemistry were monitored for three years and the woodchip and bioreactor-associated soil were analyzed for denitrification potential and chemical properties after 25 months. The in-ditch bioreactor did not significantly reduce nitrate concentrations in the ditch, likely due to low hydraulic connectivity with stream water and sedimentation. The diversion bioreactor significantly reduced nitrate concentrations (58% average reduction) but treated only ~2% of annual ditch flow. Denitrification potential was significantly higher in the in-ditch bioreactor woodchips versus the diversion bioreactor after 25 months (2950 ± 580 vs. 620 ± 310 ng N g−1 dry media h−1). The passive flow design was simple to construct and did not restrict flow in the drainage ditch but resulted in low hydraulic exchange, limiting nitrate removal.

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Denitrification behavior and microbial community spatial distribution inside woodchip-based solid-phase denitrification (W-SPD) bioreactor for nitrate-contaminated water treatment

Cited by 87 publications

References 36 publications

Insights into simultaneous microbial chromium and nitrate reduction: inhibitory effects and molecular mechanisms

Insights into simultaneous microbial chromium and nitrate reduction: inhibitory effects and molecular mechanisms

Denitrifying bioreactor microbiome: Understanding pollution swapping and potential for improved performance

Nitrate Removal and Woodchip Properties across a Paired Denitrifying Bioreactor Treating Centralized Agricultural Ditch Flows

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