Denitrification performance of
            <i>Pseudomonas fluorescens</i>
            Z03 immobilized by graphene oxide-modified polyvinyl-alcohol and sodium alginate gel beads at low temperature

Tang, Meizhen; Jiang, Jie; Lv, Qilin; Yang, Bin; Zheng, Mingna; Gao, Xiaoya; Han, Jindi; Zhang, Yingjie; Yang, Yuewei

doi:10.1098/rsos.191542

Cited by 18 publications

(6 citation statements)

References 41 publications

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“…During the measurement, due to the electron beam passing through the sample, numerous cracks were formed on the surface of the film (Figure2C,G), allowing for the observation of the internal structure of the film. Figure 3 clearly shows that the internal structure of the film is porous; the resulting nanopores are most likely due to the presence of graphene oxide [43]. The surfaces of the obtained nanocomposites have a vesicular structure, which may be due to the fact that during nanoparticle generation, the alginate formed as nanocapsules with a size of about 20-50 nm (Figure 2B,F).…”

Section: Resultsmentioning

confidence: 98%

Synthesis of Silver and Gold Nanoparticles in Sodium Alginate Matrix Enriched with Graphene Oxide and Investigation of Properties of the Obtained Thin Films

et al. 2021

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Polymer nanocomposites containing nanometals became a subject of interest due to their bactericidal properties. Different polysaccharides have been used as matrices for nanosilver and nanogold synthesis. In this study, we present a novel, environmentally friendly method for the preparation of sodium alginate/nanosilver/graphene oxide (GOX) and sodium alginate/nanogold/graphene oxide GOX nanocomposites and their characteristics. The formation of approximately 10–20 nm ball-shaped Ag and Au nanoparticles was confirmed by UV–vis spectroscopy, scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectra. The incorporation of GOX sheets within the ALG matrix improved the thermal stability of the nanocomposites film, which was measured using the differential scanning calorimetry (DSC). We also estimated the molecular weights of polysaccharide chains of the matrix with the size exclusion chromatography coupled with multiangle laser light scattering and refractometric detectors (HPSEC-MALLS-RI). The composites were more prone to enzymatic hydrolysis. The strongest bacteriostatic activity was observed for the sample containing nanosilver.

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Section: Resultsmentioning

confidence: 98%

Synthesis of Silver and Gold Nanoparticles in Sodium Alginate Matrix Enriched with Graphene Oxide and Investigation of Properties of the Obtained Thin Films

et al. 2021

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“…On the other hand, these results 10. 3389/fmicb.2022.1037626 Frontiers in Microbiology 14 frontiersin.org also suggest that facultative anaerobic microorganisms capable of performing complete denitrification, such as P. aeruginosa, P. fluorescens, P. stutzeri, and P. denitrificans, could carry out the denitrification process at higher concentrations of DO, as demonstrated in a previous study, in which the authors showed that P. stutzeri isolated from a wastewater treatment system, can carry out nitrification and denitrification simultaneously in the presence of high levels of oxygen (bottles containing an atmosphere of 92% oxygen; Su et al, 2001;Liou and Madsen, 2008;Arat et al, 2015;Tang et al, 2020). This information coincides with the fact that Pseudomonas was the most abundant genus in this study (Figure 6C).…”

mentioning

confidence: 79%

Characterizing a subtropical hypereutrophic lake: From physicochemical variables to shotgun metagenomic data

et al. 2022

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Lake Cajititlán is a subtropical and endorheic lake, which is heavily impacted by nutrient pollution. Agricultural runoff and poorly treated wastewater have entered this reservoir at alarming rates during past rainy seasons, causing the cultural eutrophication of this body of water and resulting in several massive fish kill events. In this study, shotgun metagenomic sequencing was used to examine the taxonomic and functional structure of microbial communities in Lake Cajititlán during the rainy season. Several water quality features and their interactions with microbial communities were also assessed to identify the major factors affecting the water quality and biota, specifically fish species. According to current water quality regulations, most of the physicochemical variables analyzed (dissolved oxygen, pH, Secchi disk, NH4+, NO3−, blue-green algae, total phosphorus, and chlorophyll-a) were outside of the permissible limits. Planktothrix agardhii and Microcystis aeruginosa were the most abundant phytoplankton species, and the dominant bacterial genera were Pseudomonas, Streptomyces, and Flavobacterium, with Pseudomonas fluorescens, Stenotrophomonas maltophilia, and Aeromonas veronii representing the most abundant bacterial species. All of these microorganisms have been reported to be potentially harmful to fish, and the latter three (P. fluorescens, S. maltophilia, A. veronii) also contain genes associated with pathogenicity in fish mortality (fur, luxS, aer, act, aha, exu, lip, ser). Genetic evidence from the microbial communities analyzed herein reveals that anthropogenic sources of nutrients in the lake altered genes involved in nitrogen, phosphorus, sulfur, and carbon metabolism, mainly at the beginning of the rainy season. These findings suggest that abiotic factors influence the structure of the microbial communities, along with the major biogeochemical cycles of Lake Cajititlán, resulting in temporal variations and an excess of microorganisms that can thrive in high-nutrient and low-oxygen environments. After reviewing the literature, this appears to be the first study that focuses on characterizing the water quality of a subtropical hypereutrophic lake through associations between physicochemical variables and shotgun metagenomic data. In addition, there are few studies that have coupled the metabolism of aquatic ecosystems with nutrient cycles.

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“…Stepwise denitrification of NO 3 − to N 2 gas can be facilitated by other Pseudomonas strains such as P.aeruginosa [137], P. fluorescens [138], and P. denitrificans [139], which host all of the same denitrification genes as P.stutzeri. The two bacterial genera, Pseudomoas and Paracoccus, that include strains bearing all required genes to reduce NO 3 − to NO 2 − , NO and further to gaseous N 2 O and N 2 gasses are used to benchmark denitrification kinetics under different conditions [119,135].…”

Section: Microbial Nitrogen Metabolism and Biochemical Pathwaysmentioning

confidence: 99%

Nitrate Water Contamination from Industrial Activities and Complete Denitrification as a Remediation Option

Moloantoa

Khetsha

Heerden

et al. 2022

Water

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Freshwater is a scarce resource that continues to be at high risk of pollution from anthropogenic activities, requiring remediation in such cases for its continuous use. The agricultural and mining industries extensively use water and nitrogen (N)-dependent products, mainly in fertilizers and explosives, respectively, with their excess accumulating in different water bodies. Although removal of NO3 from water and soil through the application of chemical, physical, and biological methods has been studied globally, these methods seldom yield N2 gas as a desired byproduct for nitrogen cycling. These methods predominantly cause secondary contamination with deposits of chemical waste such as slurry brine, nitrite (NO2), ammonia (NH3), and nitrous oxide (N2O), which are also harmful and fastidious to remove. This review focuses on complete denitrification facilitated by bacteria as a remedial option aimed at producing nitrogen gas as a terminal byproduct. Synergistic interaction of different nitrogen metabolisms from different bacteria is highlighted, with detailed attention to the optimization of their enzymatic activities. A biotechnological approach to mitigating industrial NO3 contamination using indigenous bacteria from wastewater is proposed, holding the prospect of optimizing to the point of complete denitrification. The approach was reviewed and found to be durable, sustainable, cost effective, and environmentally friendly, as opposed to current chemical and physical water remediation technologies.

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Denitrification performance of Pseudomonas fluorescens Z03 immobilized by graphene oxide-modified polyvinyl-alcohol and sodium alginate gel beads at low temperature

Cited by 18 publications

References 41 publications

Synthesis of Silver and Gold Nanoparticles in Sodium Alginate Matrix Enriched with Graphene Oxide and Investigation of Properties of the Obtained Thin Films

Synthesis of Silver and Gold Nanoparticles in Sodium Alginate Matrix Enriched with Graphene Oxide and Investigation of Properties of the Obtained Thin Films

Characterizing a subtropical hypereutrophic lake: From physicochemical variables to shotgun metagenomic data

Nitrate Water Contamination from Industrial Activities and Complete Denitrification as a Remediation Option

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