Densitometry Application for Evaluation of the Visualizing Agents for Dehydroepiandrosterone

Abstract

In our research the dehydroepiandrosterone (DHEA) was detected. The main aim of the work was to determine the possibility of application for the new visualizing agents for DHEA. Methylene violet, gentian violet, janus blue, methylene blue, and malachite green were used as visualizing agents. Chloramine T and rodamine B were used as comparative reagents described in literature.DHEA solution was spotted on chromatographic plates and then dipped in solutions of visualizing reagents, dried and scanned using a densitometer. The plates without using visualizing agents, as well as after using procedure described in literature (using chloramine T) were also scanned. The bands with maximum absorption (k max ) for DHEA without and with using visualizing agents were settled on the base of spectrodensitometric analysis. Consequently, the densitometric analysis of DHEA by (k max ) determined was carried out. On the base of concentration values of investigated solution of DHEA and height and areas of densitometric bands, the relationships between heights of densitomteric bands and concentrations of DHEA, as well as between areas of densitometric bands and concentrations of DHEA were settled. The values of detectability and range of quantification, as well as the angle between tangents at the inflection points to the curves of the densitometric peaks (b) were determined.

Comparison of Different Ways of Chromatographic Spots Visualization in Determination of Antyhistamine Substances

“…The aim of this work is comparison of different ways of chromatographic spot visualization in qualitative determination of cetirizine dihydrochloride, levocetirizinedihydrochloride, loratadine and desloratadine. There are many works about using the visualizing agents for many different compounds [2][3][4][5][6][7][8] . The novelty of this work is using not only solutions of visualizing agent, iodide vapour or simply UV light but also using the reaction of photo catalytic degradation causing that chromatographic spot should be more visible.…”