Density of Dermatophagoides spp. and Its Relationship with House-dust Mite Specific Serum IgE in Persistent Asthma

Abstract

<jats:p>Background: Asthma is a chronic inflammation of the bronchial tree that emerges as a response to exogenous factors, such as allergens, irritants, and infections. Some asthmatic patients had been reported having symptoms of asthma due to house-dust mites (HDM) allergen exposure. It is associated with immune responses which were increased in the form of specific Immunoglobulin E (IgE) production against HDM allergens. This case-control study aimed to determine the HDM profiles in persistent asthmatic patients, including density of mites, as well as its relationship with specific IgE anti-HDM serum levels.Materials and Methods: A total of 13 patients with persistent asthma and 12 control patients had their specific anti-HDM IgE levels examined using Immulite 2000 xpi. The house dust samples were taken and analyzed with the Fain method.Results: The results have shown that 69% of patients in the persistent asthma group and 25% of normal patients were positive for IgE anti-HDM. Dermatophagoides pteronyssinus is a predominant species with a total of 120 mites (83.9%) of 143 mites. Correlation analysis indicated a positive relationship between IgE anti-HDM levels within the serums of patients and the density of mites in the dust obtained from bedroom spaces (Spearmen Rho, R=0.35, p=0.04).Conclusion: Positive IgE anti-HDM patients in the persistent asthma group were higher (69%) than those in the clinically normal group (25%). The density of mites were dominated by D. Pteronyssinus. The bedroom-dust mites density revealed a positive correlation with serum IgE anti-HDM levels in persistent asthma patients.Keywords: asthma, density, Dermatophagoides spp.,IgE</jats:p>

Recovery of Dermatophagoides pteronyssinus (Acariformes: Astigmata: Pyroglyphidae) is influenced by vacuuming protocol, fabric type and the viability of dust mites

“…The effect of torsion forces on the transfer of mites from child seats to fabrics was considered by Clarke et al (2015a). Given the correlation between HDMs densities and HDMs allergen, specifically Der p 1 (Anasis et al, 2020), the levels of efficacy of HDMs recovery has implications for HDMs allergen sampling efficiencies. Furthermore, HDMs are also carriers of microorganisms (Hubert et al, 2021) which have the potential to negatively affect human health in cases where HDMs infest fabrics and bedding within homes.…”