Dental Pulp Stem Cell Therapy in Ischemic Stroke: A Meta-Analysis of Preclinical Studies

Wang, Hongxia; Sun, Mengjiao; Sun, Jing; Gong, Panpan; Liu, Ning; Wang, Manxia

doi:10.1016/j.jstrokecerebrovasdis.2022.106453

Cited by 8 publications

(5 citation statements)

References 33 publications

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“…Studies using MSCs derived from the pulp of human teeth in research with rats demonstrate positive results in IS models (Shi et al, 2020;Song et al, 2017;Yalvac et al, 2009). MSCs derived from dental pulp showed positive results in stroke models (Shi et al, 2020;Wang et al, 2022), and these results were not inferior to those MSCs derived from bone marrow (Song et al, 2017). Therefore, investigating the effects exerted by the injection of human dental pulp MSC in rats with focal cerebral ischemia, both from the point of view of altering neurological function and the area of cerebral ischemia, was the primary justification and objective of the present work.…”

Section: Introductionmentioning

confidence: 86%

Intravenous human dental pulp-derived mesenchymal stem cell therapy for ischemic stroke in rats: an analysis of functional and ischemic brain areas outcomes

Greggianin,

Stefani,

Malysz

et al. 2023

Neurosci Res Notes

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Cellular therapies have been implicated in treating and rehabilitating ischemic stroke (IS), involving the basic experimental and preclinical areas. Using mesenchymal stem cells (MSC) derived from human dental pulp has shown promising results in animal models, but still with mechanisms and consequences that are not entirely clear. The study aims to evaluate the effects of intravenous MSC on rats with IS regarding neurological function and histological areas of ischemia. Thirty-two male Wistar rats underwent temporary occlusion of the middle cerebral artery (TOMCA) for 60 minutes and were divided into two groups of 16 animals each. One group received dental pulp MSC intravenously, and another received saline 2 hours after TOMCA. The animals were then evaluated using the neurological functionality scales for 15 days, and at the end of the experiment period, the histological areas of cerebral ischemia were analysed. All animals presented ischemic areas and neurological deficits compatible with IS. There was partial recovery of the functionality scores over the evaluation period, and all animals presented focal cerebral ischemia measured by histological analysis; however, there was no statistical difference between the groups. The TOMCA model was effective in reproducing IS. Although we found no difference between treatment groups, our results were useful in showing the pattern of neurological recovery presented by animals treated with dental pulp MSC and the need to extend the evaluation time for a longer period and use more sensitive functional tests. The results add valuable data for improving research with dental pulp MSC in the murine model of IS.

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Section: Introductionmentioning

confidence: 86%

Intravenous human dental pulp-derived mesenchymal stem cell therapy for ischemic stroke in rats: an analysis of functional and ischemic brain areas outcomes

Greggianin,

Stefani,

Malysz

et al. 2023

Neurosci Res Notes

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“…As an important component of mesenchymal stem cells (MSCs), dental pulp-derived stem cells (DPSCs) originate in the neural crest and have significant neuroectoderm features and the potential to repair damaged tissues (Liu et al, 2015) and express NG2 (Raza et al, 2018). Due to the ease of isolation, low ethical controversy, and low immunogenicity, DPSCs transplantation has neuroprotective effects and promotes neurological function recovery after AIS (Wang et al, 2022a). For example, the administration of DPSCs that overexpress hepatocyte growth factor (HGF) during the acute phase of AIS can regulate inflammation and BBB permeability and improve brain injury (Sowa et al, 2018).…”

Section: Strategy To Regulate Ng2-glia (Fig 2c)mentioning

confidence: 99%

The NG2-glia is a potential target to maintain the integrity of neurovascular unit after acute ischemic stroke

Geng

Zhao

et al. 2023

Neurobiology of Disease

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“…The higher the activity of ANA, ACA, and ANCA antibodies, the more intense the degree of cerebrovascular blockage. 16 The cerebral pia mater is an important tissue rich in brain blood vessels and nerves, in which the blood vessels and nerve are staggered winding due to its shape characteristic, causing blood circulation disorders in patients with ACI. After ischemic necrosis and softening of brain tissue, the structure and function of the peripheral nerve also showed abnormal changes, resulting in neurological defects.…”

Section: Study On Patients With Cerebral Infarctionmentioning

confidence: 99%

Relations between antinuclear, antineutrophil cytoplasmic, anticardiolipin antibodies and degree of neurological deficit, cerebrovascular stenosis in cerebral infarction patients

Meng¹,

Xing²,

Zhang³

et al. 2023

Pak J Med Sci

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Objective: To investigate the correlation of antinuclear antibody (ANA), antineutrophil cytoplasmic antibody (ANCA) and anticardiolipin antibody (ACA) with the degree of the neurological defect and cerebrovascular stenosis in patients with cerebral infarction. Methods: Clinical data of 99 patients with acute cerebral infarction (ACI) admitted to the Department of Neurology of Baoding First Central Hospital from June 2020 to December 2021 were retrospectively analyzed, and their ANA, ACA, ANCA, neurological deficit (NIHSS) scores as well as cerebrovascular stenosis were detected and assessed. Moreover, the correlation between the positive expression rates of ANA, ANCA, ACA and the degree of the neurological deficit, as well as the location and degree of cerebrovascular stenosis, were analyzed. Results: All patients had ANA, ACA, ANCA antibodies with positive rates of 68.69%, 70.71%, 69.70%, and mild, moderate, and severe cerebrovascular stenosis with incidence rates of 28.28%, 32.32%, and 39.39% respectively; Moreover, their incidence of mild, moderate, and severe neurological deficits were 15.15%, 44.44%, and 40.40%, respectively. Statistically significant differences could be observed in the degree of cerebrovascular stenosis and neurological deficit between the ANA, ACA and ANCA antibody positive group and the negative group (p<0.05). ANA, ACA, ANCA antibody positive was moderately positively correlated with cerebrovascular stenosis rate and NIHSS score (0.40<r<0.60, p<0.05). Conclusions: The positive rates of ANA, ACA and ANCA antibodies were higher in patients with ACI, which was closely correlated with the degree of cerebrovascular stenosis and neurological deficit. doi: https://doi.org/10.12669/pjms.39.3.6765 How to cite this: Meng H, Xing X, Zhang C, Zhao J, Ren C, Liu Y. Relations between antinuclear, antineutrophil cytoplasmic, anticardiolipin antibodies and degree of neurological deficit, cerebrovascular stenosis in cerebral infarction patients. Pak J Med Sci. 2023;39(3):677-681. doi: https://doi.org/10.12669/pjms.39.3.6765 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Dental Pulp Stem Cell Therapy in Ischemic Stroke: A Meta-Analysis of Preclinical Studies

Cited by 8 publications

References 33 publications

Intravenous human dental pulp-derived mesenchymal stem cell therapy for ischemic stroke in rats: an analysis of functional and ischemic brain areas outcomes

Intravenous human dental pulp-derived mesenchymal stem cell therapy for ischemic stroke in rats: an analysis of functional and ischemic brain areas outcomes

The NG2-glia is a potential target to maintain the integrity of neurovascular unit after acute ischemic stroke

Relations between antinuclear, antineutrophil cytoplasmic, anticardiolipin antibodies and degree of neurological deficit, cerebrovascular stenosis in cerebral infarction patients

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