Pain is defined as an unpleasant sensation experienced by an individual. Non-steroidal anti-inflammatory drugs (NSAIDS) are drugs that are often used to relieve pain. Sea cucumbers are fisheries commodities that have high economic value and are very popular in the international market, one type of the sea cucumbers in Indonesia, especially found on the East coast of Surabaya, sea cucumber (Phyllophorus). In several studies, it has been proven that sea cucumber flavonoids have anti-inflammatory effects. This study uses a post-test only control group design. This study used 25 mice, which were divided into 5 groups. Group 1 was given aquades 10mL/kg BW, group 2 was given acetosal 150mg/kg BW, group 3 was given sea cucumber extract dose 20mg/kg BW, group 4 was given sea cucumber extract 40mg/kg BW, and group 5 was given sea cucumber extract 80mg/kg BW. Each group was induced with pain with acetic acid intraperitoneum 0.7% dose of 10ml/kg BW. Wait 5 minutes. Then do observation and calculation the mice stretching every 10 minutes. The data are taken observation and calculation 3×. Descriptive analysis results showed a decrease in the average stretching of mice in the acetosal therapy group 150mg/kg BW and better than another groups. The results of statistical analysis with Mann-Whitney U showed that there were significant differences (p<0.05) between the positive control group and the treatment group dose of 20mg/kg BW (p<0.05); positive control group and the treatment group dose 80mg/kg BW (p<0.05); the treatment group dose 20mg/kg BW and the treatment group dose 40mg/kg BW (p<0.05). From these results, it can be concluded that the sea cucumber extract (Phyllophorus sp.) Dose 40 mg/kg BW has better analgesic effect compared to the treatment group with doses of 20mg/kg BW and 80mg/kg BW but not better than the positive control group.
