Dentin Matrix Protein-1 Activates Dental Pulp Fibroblasts

Abd-Elmeguid, Ashraf; Yu, Donald C.; Kline, Loren; Moqbel, Redwan; Vliagoftis, Harissios

doi:10.1016/j.joen.2011.10.005

Cited by 34 publications

(37 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A wide variety of cytokines, growth factors and oncogene products stimulate MMP expression [13], [14]. Tumor necrosis factor (TNF)-α and interleukin (IL)-1 are regularly involved in metalloproteinase gene induction [15]–[18]. Although we previously reported that a proinflammatory cytokine mixture (IL-1β, TNF-α, and IFN-γ) could induce MMP-3 activity in odontoblast-like cells derived from mouse embryonic stem (ES) cells [19], the identification of the principal cytokine responsible for this MMP-3 stimulus remains unresolved.…”

Section: Introductionmentioning

confidence: 99%

Matrix Metalloproteinase-3 in Odontoblastic Cells Derived from Ips Cells: Unique Proliferation Response as Odontoblastic Cells Derived from ES Cells

et al. 2013

View full text Add to dashboard Cite

We previously reported that matrix metalloproteinase (MMP)-3 accelerates wound healing following dental pulp injury. In addition, we reported that a proinflammatory cytokine mixture (tumor necrosis factor-α, interleukin (IL)-1β and interferon-γ) induced MMP-3 activity in odontoblast-like cells derived from mouse embryonic stem (ES) cells, suggesting that MMP-3 plays a potential unique physiological role in wound healing and regeneration of dental pulp in odontoblast-like cells. In this study, we tested the hypothesis that upregulation of MMP-3 activity by IL-1β promotes proliferation and apoptosis of purified odontoblast-like cells derived from induced pluripotent stem (iPS) and ES cells. Each odontoblast-like cell was isolated and incubated with different concentrations of IL-1β. MMP-3 mRNA and protein expression were assessed using RT-PCR and western blotting, respectively. MMP-3 activity was measured using immunoprecipitation and a fluorescence substrate. Cell proliferation and apoptosis were determined using ELISA for BrdU and DNA fragmentation, respectively. siRNA was used to reduce MMP-3 transcripts in these cells. Treatment with IL-1β increased MMP-3 mRNA and protein levels, and MMP-3 activity in odontoblast-like cells. Cell proliferation was found to markedly increase with no changes in apoptosis. Endogenous tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2 were constitutively expressed during all experiments. The exocytosis inhibitor, Exo1, potently suppressed the appearance of MMP-3 in the conditioned medium. Treatment with siRNA against MMP-3 suppressed an IL-1β-induced increase in MMP-3 expression and activity, and also suppressed cell proliferation, but unexpectedly increased apoptosis in these cells (P<0.05). Exogenous MMP-3 was found to induce cell proliferation in odontoblast-like cells derived from iPS cells and ES cells. This siRNA-mediated increase in apoptosis could be reversed with exogenous MMP-3 stimulation (P<0.05). Taken together, IL-1β induced MMP-3-regulated cell proliferation and suppressed apoptosis in odontoblast-like cells derived from iPS and ES cells.

show abstract

Section: Introductionmentioning

confidence: 99%

Matrix Metalloproteinase-3 in Odontoblastic Cells Derived from Ips Cells: Unique Proliferation Response as Odontoblastic Cells Derived from ES Cells

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Pulp fibroblasts were characterized by their morphology and were analysed by flow cytometry for their expression of vimentin and their lack of CD34, CD45 and cytokeratin immunoreactivity (Abd‐Elmeguid et al . ). MRC5 sound fibroblasts were cultured under the same conditions.…”

Section: Methodsmentioning

confidence: 97%

Expression of factors involved in dental pulp physiopathological processes by nemotic human pulpal fibroblasts

et al. 2017

View full text Add to dashboard Cite

Le Clerc J, Tricot-Doleux S, Pellen-Mussi P, P erard M, Jeanne S, P erez F. Expression of factors involved in dental pulp physiopathological processes by nemotic human pulpal fibroblasts. International Endodontic Journal, 51, e94-e106, 2018.Aim To investigate in human dental pulp fibroblasts (HDPF) the expression of factors involved in dental pulp physiopathological processes and in an experimental model of cell activation called nemosis, and to compare the behaviour of pulp cell activation with sound lung fibroblast MRC5, employed as a reference model for nemosis. Methodology Nemotic response was induced in three-dimensional cultures of HDPF and lung fibroblasts. The expressions of molecules involved in physiological (alkaline phosphatase, type I collagen) and in inflammatory processes (IL-6, CXCL8, CCL20, COX-2) were studied using real-time PCR. Concentrations of IL-6 and CXCL8 were analysed during 4 days with ELISA. Nonparametric tests were used to determine statistical differences between groups. Results A significant decrease (P < 0.001) in type I collagen and alkaline phosphatase was observed in MRC5 and HDPF nemotic responses. Although the amounts of mRNA differed between these cell types, there was an increase in CCL20, CXCL8 and COX-2 expression (P < 0.001). Unlike HDPF, MRC5 spheroids displayed significant amounts of IL-6 concentrations and mRNA expression. Notably, increased concentrations of CXCL8 were recorded in all threedimensional cultures compared with monolayers as a function of time (P < 0.05). Conclusion Although the nemotic responses observed were not identical in the pulpal and lung fibroblasts, similarities occurred in the expression of chemokines and cyclooxygenase-2. Nemotic reactions and inflammatory processes in pulp diseases share similarities in terms of the expression of factors. Thus, this in vitro model could constitute a powerful tool to study intercellular relations within the dental pulp and to develop new local treatments to counteract the inflammatory reaction that occurs during pulpitis.

show abstract

“…Pulp cells were characterized as fibroblasts by their morphology and staining for procollagen I 1 and fibroblast surface protein 30 by means of immunostaining. Fibroblasts from permanent and deciduous teeth were seeded over sterile 12 mm round cover slips (BD Biosciences, Bedford, USA).…”

Section: Methodsmentioning

confidence: 99%

CCL3 and CXCL12 production in vitro by dental pulp fibroblasts from permanent and deciduous teeth stimulated by Porphyromonas gingivalis LPS

et al. 2013

View full text Add to dashboard Cite

Objective:The aim of this study was to compare the production of the chemokines CCL3 and CXCL12 by cultured dental pulp fibroblasts from permanent (PDPF) and deciduous (DDPF) teeth under stimulation by Porphyromonas gingivalis LPS (PgLPS).Material and Methods:Primary culture of fibroblasts from permanent (n=3) and deciduous (n=2) teeth were established using an explant technique. After the fourth passage, fibroblasts were stimulated by increasing concentrations of PgLPS (0 - 10 µg/mL) at 1, 6 and 24 h. The cells were tested for viability through MTT assay, and production of the chemokines CCL3 and CXCL12 was determined through ELISA. Comparisons among samples were performed using One-way ANOVA for MTT assay and Two-way ANOVA for ELISA results. Results:Cell viability was not affected by the antigen after 24 h of stimulation. PgLPS induced the production of CCL3 by dental pulp fibroblasts at similar levels for both permanent and deciduous pulp fibroblasts. Production of CXCL12, however, was significantly higher for PDPF than DDPF at 1 and 6 h. PgLPS, in turn, downregulated the production of CXCL12 by PDPF but not by DDPF.Conclusion:These data suggest that dental pulp fibroblasts from permanent and deciduous teeth may present a differential behavior under PgLPS stimulation.

show abstract

Dentin Matrix Protein-1 Activates Dental Pulp Fibroblasts

Cited by 34 publications

References 40 publications

Matrix Metalloproteinase-3 in Odontoblastic Cells Derived from Ips Cells: Unique Proliferation Response as Odontoblastic Cells Derived from ES Cells

Matrix Metalloproteinase-3 in Odontoblastic Cells Derived from Ips Cells: Unique Proliferation Response as Odontoblastic Cells Derived from ES Cells

Expression of factors involved in dental pulp physiopathological processes by nemotic human pulpal fibroblasts

CCL3 and CXCL12 production in vitro by dental pulp fibroblasts from permanent and deciduous teeth stimulated by Porphyromonas gingivalis LPS

Contact Info

Product

Resources

About