Dentine biomodification by sulphonamides pre-treatment: bond strength, proteolytic inhibition, and antimicrobial activity

Abstract

We evaluated the effects of dentine biomodification after pre-treatment with two sulphonamide carbonic anhydrase inhibitors (CAIs) of the N-[4-sulphamoylphenethylcarbamoyl]benzenesulphonamide type, investigating matrix metalloproteases activity, resin–dentine micro tensile bond strength, dentine surface wettability, and antimicrobial activities. Ninety-five sound-extracted human molars were selected for the study. Inhibitory effects were evaluated by gelatinase and collagenase activity tests and collagen degradation FT-IR spectroscopic analysis. Pre-treatment with the two CAIs kept the micro tensile values after 12 months of storage (32.23 ± 5.95) and cariogenic challenge (34.13 ± 2.71) similar to the initial, pre-treatment values (33.56 ± 4.34). A decreased Streptococcus mutans biofilm formation on dentine surfaces and antibacterial activity against planktonic bacteria were observed after CAI treatment. Dentine pre-treatment with sulphonamide CAIs maintained adhesion strength stability, allowed better dentine wettability, maintained matrix collagen, and showed anti- S. mutans activity.

2-(N-allylsulfamoyl)-N-propylbenzamide

“…Sulfonamides are a very interesting class of drugs because of their many pharmacological properties [1], including their ability to inhibit carbonic anhydrase "CA" and their diuretic [2,3], anticancer [4], hypoglycemic [5], antiviral [6], antibacterial [7], and metalloprotease inhibitory effects [8]. Although they were discovered in the 1930s as chemotherapeutic agents for their antibacterial properties, they have recently attracted increasing interest due to the discovery of new pharmacological properties [9].…”