Deoxycholic acid inhibits Staphylococcus aureus-induced endometritis through regulating TGR5/PKA/NF-κB signaling pathway

Zhao, Weiliang; Wang, Junrong; Li, Xiaojie; Li, Yang; Ye, Cong

doi:10.1016/j.intimp.2023.110004

Cited by 10 publications

(4 citation statements)

References 31 publications

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“… 28 S. aureus is the main bacterium in endometritis. 29 The mouse endometritis model induced by intrauterine infusion of S. aureus has been widely used to explore the pathological mechanism of endometritis. 30 , 31 Therefore, this study used mouse model of endometritis induced by S. aureus for the experiment.…”

Section: Discussionmentioning

confidence: 99%

Puerarin inhibits Staphylococcus aureus‐induced endometritis through attenuating inflammation and ferroptosis via regulating the P2X7/NLRP3 signalling pathway

Yuan,

Liu,

Zhao

et al. 2024

J Cellular Molecular Medi

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Endometritis is one of the important causes of infertility. Puerarin (PU) can inhibit oxidative stress and reduce inflammation; however, it is unclear whether PU has a protective effect on the endometritis. In our study, we used Staphylococcus aureus to induce mouse endometritis. The PU group (100 mg/kg PU) and the S. aureus + PU group received daily intraperitoneal injection of PU (25, 50 or 100 mg/kg PU). The results showed that S. aureus significantly increased the levels of MPO, TNF‐α, IL‐1β and IL‐6 in uterine tissue, and increased the expression of p‐p65 and p‐IκBα proteins in uterine tissue to induce endometritis in mice (p < 0.05). Furthermore, it has been found that S. aureus promotes the occurrence of ferroptosis by reducing GSH and ATP content, increasing MDA and iron content and reducing GPX4 and SLC7A11 protein expression levels (p < 0.05). S. aureus significantly increase the expression of NLRP3, ASC, caspase‐1 and P2X7 proteins in uterine tissue (p < 0.05). However, PU obviously reduced the inflammatory response and reversed the changes of ferroptosis and the expression of P2X7 receptor/NLRP3 pathway associated proteins of the uterus induced by S. aureus (p < 0.05). Taken together, these findings emphasize the protective effect of PU on endometritis by regulating the P2X7 receptor/NLRP3 signalling pathway and inhibiting ferroptosis.

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Section: Discussionmentioning

confidence: 99%

Puerarin inhibits Staphylococcus aureus‐induced endometritis through attenuating inflammation and ferroptosis via regulating the P2X7/NLRP3 signalling pathway

Yuan,

Liu,

Zhao

et al. 2024

J Cellular Molecular Medi

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“…Nrf2-knockout (Nrf2 −/− ) mice on a C57BL/6J background were purchased from the Model Animal Research Center (Nanjing, China). Sixty mice were divided into five groups: (i) control group: the mice received equal amounts of vehicle; (ii) S. aureus group: the mice received 50 µL of S. aureus (1 × 10 7 CFU per 50 µL PBS) to each side of the mouse uterus ( 29 ); and (iii–v) S. aureus +luteolin (10, 20, and 40 mg/kg) groups: the mice were given luteolin (10, 20, and 40 mg/kg) intraperitoneally 1 h before S. aureus treatment. Twenty-four hours later, the mice were sacrificed, and the uterine tissues were collected for subsequent experiments.…”

Section: Methodsmentioning

confidence: 99%

Luteolin attenuates Staphylococcus aureus -induced endometritis through inhibiting ferroptosis and inflammation via activating the Nrf2/GPX4 signaling pathway

Gao,

Cai

et al. 2024

Microbiol Spectr

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Endometritis, a local inflammatory disease, has been known as the most common cause of infertility in mares. In this study, we investigated the protective effects of luteolin on endometritis induced by Staphylococcus aureus ( S. aureus ) and further clarified the possible molecular mechanisms. An S. aureus –induced endometritis model was established by the infusion of S. aureus into the uterus. Luteolin was intraperitoneally administered to mice 1 h before S. aureus treatment. The results showed that the mice of the S. aureus group showed severe histological changes of uterine tissues, increased myeloperoxidase (MPO) activity, and elevated TNF-α, IL-1β, and IL-6 levels. These changes induced by S. aureus were dose-dependently inhibited by luteolin. Furthermore, luteolin inhibited MDA and Fe 2+ production and increased the production of GSH decreased by S. aureus . Luteolin prevented S. aureus –induced endometrial barrier disruption through up-regulating ZO-1 and occludin expression. Luteolin dramatically inhibited S. aureus –induced NF-κB activation. The expression of Nrf2 and HO-1 was increased by luteolin. In addition, the inhibitory effects of luteolin on S. aureus –induced endometritis were reversed in Nrf2 knockdown mice. In conclusion, these data indicated that luteolin protected mice against S. aureus –induced endometritis through inhibiting inflammation and ferroptosis via regulating the Nrf2 signaling pathway. IMPORTANCE Endometritis is an inflammatory disease of the endometrium, which is a common gynecological disease. Up to now, there is no evidence for the protective effects of luteolin on endometritis. The purpose of this study was to investigate whether luteolin has protective effects against S. aureus –induced endometritis and attempts to clarify the mechanism.

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“…CA plays a critical role in regulating cholesterol metabolism and the uptake of fat-soluble vitamins in the small intestine, 9,10 while DCA is medically used for treating cholesterol gallstones, endometritis, cardiometabolic diseases, and as a tumor biomarker. 9,11,12…”

Section: Introductionmentioning

confidence: 99%

“…See DOI: https://doi.org/10.1039/d3gc03095d treating cholesterol gallstones, endometritis, cardiometabolic diseases, and as a tumor biomarker. 9,11,12 Industrial production of DCA involves extraction from animal bile acids-where it co-exists with CA 13 -or by removing the hydroxyl group on the 7th carbon atom of CA via 7α-dehydroxylase, 14 a process which also generates a CA-DCA mixture. Given that the only structural difference between CA and DCA is the hydroxyl group on the 7-position carbon atom, effective isolation of CA and DCA from complex biological extracts or synthetic reaction mixtures has remained highly difficult using conventional separation techniques.…”

Section: Introductionmentioning

confidence: 99%

A synergistic ‘push and pull’ ionic liquid biphasic system for enhanced extraction separation of cholic acid and deoxycholic acid

Ding,

Rong,

Cao

et al. 2023

Green Chem.

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Deoxycholic acid inhibits Staphylococcus aureus-induced endometritis through regulating TGR5/PKA/NF-κB signaling pathway

Cited by 10 publications

References 31 publications

Puerarin inhibits Staphylococcus aureus‐induced endometritis through attenuating inflammation and ferroptosis via regulating the P2X7/NLRP3 signalling pathway

Puerarin inhibits Staphylococcus aureus‐induced endometritis through attenuating inflammation and ferroptosis via regulating the P2X7/NLRP3 signalling pathway

Luteolin attenuates Staphylococcus aureus -induced endometritis through inhibiting ferroptosis and inflammation via activating the Nrf2/GPX4 signaling pathway

A synergistic ‘push and pull’ ionic liquid biphasic system for enhanced extraction separation of cholic acid and deoxycholic acid

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