Deoxynivalenol resistance as a component of FHB resistance

Gunupuru, Lokanadha Rao; Perochon, Alexandre; Doohan, Fiona M.

doi:10.1007/s40858-017-0147-3

Cited by 55 publications

(63 citation statements)

References 74 publications

(87 reference statements)

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“…Because the flowering patterns between genotypes were similar in the years analyzed, these differences in mycotoxin contamination could be a result of differences in FHB resistance (polygenic resistance). 34 In 2014, we expected a higher mycotoxin contamination compared to 2015 and 2016, related to the major values of incidence/FHB index observed in the field. In 2014, we observed a slight predominance of F. graminearum respect to F. poae; therefore, we expected to find a major proportion of DON and its acetylated derivatives compared to NIV.…”

Section: Discussionmentioning

confidence: 80%

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Effects of Fusarium graminearum and Fusarium poae on disease parameters, grain quality and mycotoxins contamination in bread wheat (Part I)

et al. 2019

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BACKGROUND Wheat is the most important winter crop in the world, being affected by the presence of fungal, mainly those belonging to the Fusarium genus. Fusarium head blight (FHB) is a serious disease that causes important economic damage and quantitative/qualitative losses, with Fusarium graminearum and Fusarium poae being two of the most isolated species worldwide. The present study aimed to evaluate the interaction between F. graminearum and F. poae and the effects on disease parameters, grain quality and mycotoxin contamination on five wheat genotypes under field conditions during three growing seasons. RESULTS Statistical differences between Fusarium treatments were found for disease parameters, grain quality and mycotoxin contamination during the 2014/2015 growing season. High values of incidence (58.00 ± 8.00%), severity (6.28 ± 1.51%) and FHB index (4.72 ± 1.35) were observed for F. graminearum + F. poae treatment. Regarding grain quality, the results showed that the degradation of different protein fractions depends on each Fusarium species: glutenins were degraded preferably by F. graminearum (−70.82%), gliadins were degraded preferably by F. poae (−29.42%), whereas both protein fractions were degraded when both Fusarium species were present (−60.91% and −16.51%, respectively). Significant differences were observed for mycotoxin contamination between genotypes, with Proteo being the most affected (DON = 12.01 ± 3.67 μg g−1). In addition, we report that 3‐ADON predominated over 15‐ADON in the three seasons evaluated. CONCLUSION Variations in plant–pathogen interaction (Fusarium–wheat pathosystem) should be considered at least in years with favorable climatic conditions for FHB development, as a result of the potential impact of this disease on grain quality and mycotoxin contamination. © 2019 Society of Chemical Industry

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Section: Discussionmentioning

confidence: 80%

“…), showing different extents of mycotoxin contamination in each genotype. Because the flowering patterns between genotypes were similar in the years analyzed, these differences in mycotoxin contamination could be a result of differences in FHB resistance (polygenic resistance) …”

Section: Discussionmentioning

confidence: 99%

Effects of Fusarium graminearum and Fusarium poae on disease parameters, grain quality and mycotoxins contamination in bread wheat (Part I)

et al. 2019

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“…DON resistance is a component of FHB resistance (Gunupuru et al, 2017). We hypothesized that like TaFROG, TaNACL-D1 might enhance both FHB and DON resistance .…”

Section: Tanacl-d1 Enhances Fhb Resistancementioning

confidence: 99%

A wheat NAC interacts with an orphan protein and enhances resistance to Fusarium head blight disease

Perochon

Kahla

Vranić

et al. 2019

Plant Biotechnology Journal

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Summary Taxonomically‐restricted orphan genes play an important role in environmental adaptation, as recently demonstrated by the fact that the Pooideae‐specific orphan TaFROG (Triticum aestivum Fusarium Resistance Orphan Gene) enhanced wheat resistance to the economically devastating Fusarium head blight (FHB) disease. Like most orphan genes, little is known about the cellular function of the encoded protein TaFROG, other than it interacts with the central stress regulator TaSnRK1α. Here, we functionally characterized a wheat (T. aestivum) NAC–like transcription factor TaNACL‐D1 that interacts with TaFROG and investigated its’ role in FHB using studies to assess motif analyses, yeast transactivation, protein‐protein interaction, gene expression and the disease response of wheat lines overexpressing TaNACL‐D1. TaNACL‐D1 is a Poaceae‐divergent NAC transcription factor that encodes a Triticeae‐specific protein C‐terminal region with transcriptional activity and a nuclear localisation signal. The TaNACL‐D1/TaFROG interaction was detected in yeast and confirmed in planta, within the nucleus. Analysis of multi‐protein interactions indicated that TaFROG could form simultaneously distinct protein complexes with TaNACL‐D1 and TaSnRK1α in planta. TaNACL‐D1 and TaFROG are co‐expressed as an early response to both the causal fungal agent of FHB, Fusarium graminearum and its virulence factor deoxynivalenol (DON). Wheat lines overexpressing TaNACL‐D1 were more resistant to FHB disease than wild type plants. Thus, we conclude that the orphan protein TaFROG interacts with TaNACL‐D1, a NAC transcription factor that forms part of the disease response evolved within the Triticeae.

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“…Several studies have associated the ability to conjugate DON into DON‐3‐ O ‐glucose (D3G) with partial resistance towards FHB (for a review, see Gunupuru et al ., ). In particular, Lemmens et al .…”

Section: Introductionmentioning

confidence: 99%

“…Several studies have associated the ability to conjugate DON into DON-3-O-glucose (D3G) with partial resistance towards FHB (for a review, see Gunupuru et al, 2017). In particular, Lemmens et al (2005) showed that the presence of the Fhb1 locus was correlated with increased metabolizing of DON into D3G.…”

Section: Introductionmentioning

confidence: 99%

The Brachypodium distachyon UGT Bradi5gUGT03300 confers type II fusarium head blight resistance in wheat

et al. 2018

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Fusarium head blight (FHB), caused by fungi belonging to the Fusarium genus, is a widespread disease of wheat (Triticum aestivum) and other small‐grain cereal crops. The main causal agent of FHB, Fusarium graminearum, produces mycotoxins mainly belonging to type B trichothecenes, such as deoxynivalenol (DON), that can negatively affect humans, animals and plants. DON detoxification, mainly through glucosylation into DON‐3‐O‐glucose, has been correlated with resistance to FHB. A UDP‐glucosyltransferase from the model cereal species Brachypodium distachyon has been shown to confer resistance both to initial infection and to spike colonization (type I and type II resistances, respectively). Here, the functional characterization of transgenic wheat lines expressing the Bradi5g03300 UGT gene are described. The results show that, following inoculation with the fungal pathogen, these lines exhibit a high level of type II resistance and a strong reduction of mycotoxin content. In contrast, type I resistance was only weakly observed, although previously seen in B. distachyon, suggesting the involvement of additional host‐specific characteristics in type I resistance. This study contributes to the understanding of the functional relationship between DON glucosylation and FHB resistance in wheat.

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Deoxynivalenol resistance as a component of FHB resistance

Cited by 55 publications

References 74 publications

Effects of Fusarium graminearum and Fusarium poae on disease parameters, grain quality and mycotoxins contamination in bread wheat (Part I)

Effects of Fusarium graminearum and Fusarium poae on disease parameters, grain quality and mycotoxins contamination in bread wheat (Part I)

A wheat NAC interacts with an orphan protein and enhances resistance to Fusarium head blight disease

The Brachypodium distachyon UGT Bradi5gUGT03300 confers type II fusarium head blight resistance in wheat

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