Deoxyribonucleic Acid Relatedness of Proteus and Providencia Species

Abstract

Deoxyribonucleic acid reassociation was used to determine relatedness among protei and providenciae and between these organisms and other members of the family Entero bacteriaceae. Ewing described four biogroups in Prov. alcalifaciens and two biogroups in Prov. stuartii. These are based on the production of gas from glucose and the fermentation of adonitol and inositol (17; see Table 12).Several other taxonomic proposals for Proteus and Providencia have been reviewed by Rauss (38). The best known of these is Kauffmann's recommendation of four genera: Proteus, Morganella, Rettgerella, and Providencia (26). Kauffmann included Prot. mirabilis and Prot. vulgaris in the species Prot. hauseri. The fourgenus concept with Prot. hauseri was endorsed by Rauss (38)) and Coetzee (7) endorsed the four-genus system while retaining P. mira bizis and P. vulgaris. In a review (7), Coetzee documented serological differences in enzymes among protei. Therefore, there are antigenic, structural, and biochemical data that suggest genetic divergence among protei. With the exception of Prot. morganii (50 mol% guanine plus cytosine [G+C] in the deoxyribonucleic acid [DNA]), Proteus and Prouidencia species have substantially lower G+C ratios than other Entero bacteriaceae, whose DNA contains 50 to 58 mol% G+C. Prot. mirabilis and Prot. vulgaris contain 38 to 40 mol% G+C (19, 23, 29), Prot. rettgeri has 39 to 41.5 mol% G+C, and providenciae are reported to have 40 to 42 mol% G+C. 269

Characterization of Enterobacteria by Esterase Specific-Activity Profiles

“…However, these strains, which included several null alleles, were distinguished from other species by other esterases (Goullet & Picard, 1985). The esterase data for Providencia were in agreement with the genetic divergence measured by DNA reassociation studies (Brenner et al, 1978). Group 6.…”

Providencia thailandensis sp. nov., isolated from seafood processing wastewater

“…The DNA-DNA hybridization study revealed that the strain C1112 T showed DNA-DNA similarities of 100, 48.1, 42.3, 22.6, 38.7, 39.1, 36.6, 19 (Brenner et al, 1978;Hickman-Brenner et al, 1983;Juneja and Lazzaro, 2009;Muller et al, 1986;Somvanshi et al, 2006). In addition, the results from the DNA-DNA hybridizations fall well below the 70% reassociation threshold recommendation of Wayne et al (1987) for designation of a novel species.…”

Rare occurrence of Proteus vulgaris in faeces: a reason for its rare association with urinary tract infections

“…morgani) ( I -5%) (Senior, 1979). DNA hybridisation studies (Brenner et al, 1978), urease isoenzyme profiles (Senior et al, 1980) and the sharing of phage (Coetzee, 1963) and bacteriocin receptors…”