Dependence of albumin–fibrinogen simple and competitive adsorption on surface properties of biomaterials

Abstract

SYNOPSISAdsorption of fibrinogen and albumin from solutions of the proteins singly and in mixtures was studied. A variety of surfaces with varying degrees of hydrophilicity and thrombogenicity was investigated. Adsorption behavior shows a strong dependence on surface properties. All surfaces show a preference for fibrinogen, and the degree of preference appears to correlate with thrombogenic tendency and platelet reactivity. EXPERIMENTALAdsorption was studied by radioiodine labeling of proteins using the iodine monochloride method, as described elsewhere [6]. The use of both

Are there sufficient standards for the in vitro hemocompatibility testing of biomaterials?

“…In order to understand which specific plasma proteins are primarily adsorbed by respective surfaces, adsorption profiles of proteins can be recorded using ELISA and/or western blotting techniques [16,[108][109][110][111]. Further comprehensive test collections reported by Seyfert et al comprise tests for the following aspects: contact activation, fibrinogen-fibrin conversion, fibrinolysis, hemolysis, proteolysis, and platelet activation [29].…”

Competitive adsorption of fibrinogen and albumin and blood platelet adhesion on surfaces modified with nanoparticles and/or PEO

“…Adsorption of fibrinogen from plasma plays a central role in the coagulation pathway [4][5][6][7][8], and fibrinogen was reported to be the major plasma protein mediating platelet adhesion [9]. Adsorption of fibrinogen in competition with other plasma proteins such as albumin and immunoglobulins was thus investigated [10][11][12][13]. For example, dual radiolabeling was used to directly the fibrinogen adsorbed amount is higher on hydrophobic compared to hydrophilic surfaces [18][19][20].…”

Blood compatibility of fluorodiol‐containing polyurethanes

“…Albumin has thrombo‐resistant ability, while fibrinogen promotes platelet adhesion onto the polymer surface 41, 42. Hence, the polymers having surfaces exhibiting low fibrinogen/albumin molar adsorption ratios (F/A ratios) would possess good blood compatibility and the potential for use as biomaterials 43–45…”