Dependence of Purple Membrane Bump Curvature on pH and Ionic Strength Analyzed Using Atomic Force Microscopy Combined with Solvent Exchange

Yokoyama, Yasunori; Yamada, Kosuke; Higashi, Yosuke; Ozaki, Satoshi; Wang, Haorang; Koito, Naoki; Watanabe, Nobuhisa; Sonoyama, Masashi; Mitaku, Shigeki

doi:10.1021/jp5036234

Cited by 8 publications

(2 citation statements)

References 40 publications

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“…Bacteriorhodopsin is found in highly curved, specialized membrane microdomains (purple membranes), in which it forms trimeric, hexagonal units packed in a 2D crystalline lattice together with archaeal lipids [ 144 , 145 ]. These lipids also contribute to the 2D crystalline packing as bacteriorhodopsin mutants with a constitutive wedge-shaped structure still need a specific lipidic environment to induce membrane curvature [ 146 , 147 ]. Therefore, both phospholipid composition and protein tertiary 3D structure work together to modulate the membrane curvature in purple membranes.…”

Section: Mechanisms Of Protein-induced Membrane Curvaturementioning

confidence: 99%

Inducible intracellular membranes: molecular aspects and emerging applications

et al. 2020

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Membrane remodeling and phospholipid biosynthesis are normally tightly regulated to maintain the shape and function of cells. Indeed, different physiological mechanisms ensure a precise coordination between de novo phospholipid biosynthesis and modulation of membrane morphology. Interestingly, the overproduction of certain membrane proteins hijack these regulation networks, leading to the formation of impressive intracellular membrane structures in both prokaryotic and eukaryotic cells. The proteins triggering an abnormal accumulation of membrane structures inside the cells (or membrane proliferation) share two major common features: (1) they promote the formation of highly curved membrane domains and (2) they lead to an enrichment in anionic, cone-shaped phospholipids (cardiolipin or phosphatidic acid) in the newly formed membranes. Taking into account the available examples of membrane proliferation upon protein overproduction, together with the latest biochemical, biophysical and structural data, we explore the relationship between protein synthesis and membrane biogenesis. We propose a mechanism for the formation of these non-physiological intracellular membranes that shares similarities with natural inner membrane structures found in α-proteobacteria, mitochondria and some viruses-infected cells, pointing towards a conserved feature through evolution. We hope that the information discussed in this review will give a better grasp of the biophysical mechanisms behind physiological and induced intracellular membrane proliferation, and inspire new applications, either for academia (high-yield membrane protein production and nanovesicle production) or industry (biofuel production and vaccine preparation).

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Section: Mechanisms Of Protein-induced Membrane Curvaturementioning

confidence: 99%

Inducible intracellular membranes: molecular aspects and emerging applications

et al. 2020

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“…Bacteriorhodopsin is found in highly curved, specialized membrane microdomains (purple membranes), in which it forms trimeric, hexagonal units packed in a 2D crystalline lattice together with archaeal lipids (Oesterhelt and Stoeckenius 1971;Henderson 1977). These lipids also contribute to the 2D crystalline packing as bacteriorhodopsin mutants with a constitutive wedge-shaped structure still need a specific lipidic environment to induce membrane curvature (Krebs and Isenbarger 2000;Rhinow and Hampp 2010;Yokoyama et al 2014). Therefore, both phospholipid composition and protein tertiary 3D structure work together to modulate the membrane curvature in purple membranes.…”

Section: Insertion Of Wedge-shaped Proteins Into the Membranementioning

confidence: 99%

Intracellular Membranes: Conserved Mechanisms of Formation and Regulation Throughout Evolution

Mir¹,

Biou²,

Dautin³

et al. 2020

Preprint

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Membrane remodeling and phospholipid biosynthesis are normally tightly regulated to maintain the shape and function of cells. Indeed, different physiological mechanisms ensure a precise coordination between de novo phospholipid biosynthesis and modulation of membrane morphology. Interestingly, the overproduction of certain membrane proteins hijack these regulation networks, leading to the formation of impressive intracellular membrane structures in both prokaryotic and eukaryotic cells. The proteins triggering membrane proliferation share two major common features: 1) they promote the formation of highly curved membrane domains and 2) they lead to an enrichment in anionic, cone-shaped phospholipids (cardiolipin or phosphatidic acid) in the newly formed membranes. Taking into account the available examples of membrane proliferation upon protein overproduction, together with the latest biochemical, biophysical and structural data, we explore the relationship between protein synthesis and membrane biogenesis. We propose a mechanism for the formation of these non-physiological intracellular membranes that shares similarities with natural inner membrane structures found in α-proteobacteria, mitochondria and some viruses-infected cells, pointing towards a conserved feature through evolution. We hope that the information discussed in this review will give a better grasp of the biophysical mechanisms behind physiological and induced intracellular membrane proliferation, inspiring new biotechnological applications.

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Boolean Logic Gate Operation in Bacteriorhodopsin of Purple Membrane Based on a Molten Globule‐like State

Mostafa

2024

ChemPhysChem

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Bacteriorhodopsin (bR) of purple membrane (PM) has increasing technical interests, particularly in photonic devices and bioelectronics. The present work has concerned with monitoring the temperature dependence of passive electric responses in‐plane and out‐of‐plane of the membranes. Based on thermal properties observed orthogonally here for PM, a high‐temperature intermediate of bR has been suggested to populate at around 60 °C, which may be ascribed to a molten globule‐like state. This intermediate has been found to be enclosed between two reversible thermal transitions for PM. Large‐scale turnover in the energy of activation, for these two thermal transitions, occurs steeply at such state at 60 °C, above which does bR reverse the sign of dielectric anisotropy (i.e. crossover) provided the operating frequency should be above the crossover frequency, at which the reversal occurs. No such crossover was found to occur below the crossover frequency, even above the crossover temperature (i.e. 60 °C). Likewise, no such crossover was found to occur below the crossover temperature, even above the crossover frequency. Relying on this reasoning, a logic gate operation may be declared implicating bR for bioelectronics and sense technological relevance. In addition, the results specify “dual frequency” as well as “dual temperature” characteristics to bacteriorhodopsin.

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Dependence of Purple Membrane Bump Curvature on pH and Ionic Strength Analyzed Using Atomic Force Microscopy Combined with Solvent Exchange

Cited by 8 publications

References 40 publications

Inducible intracellular membranes: molecular aspects and emerging applications

Inducible intracellular membranes: molecular aspects and emerging applications

Intracellular Membranes: Conserved Mechanisms of Formation and Regulation Throughout Evolution

Boolean Logic Gate Operation in Bacteriorhodopsin of Purple Membrane Based on a Molten Globule‐like State

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