Dependency of the [18f]fluoromisonidazole uptake on oxygen delivery and tissue oxygenation in the porcine liver

Abstract. Purpose: The purpose of this study was to assess the potential and utility of ultra-high-resolution hypoxia imaging in various murine tumour models using the established hypoxia PET tracer [ 18 F] FMISO PET imaging allowed clear-cut visualisation of the tumours. Inter-and intratumoural heterogeneity of tracer uptake was evident. In addition to average TMRR (tumour-to-muscle retention ratio including all voxels in a volume of interest (VOI)), the parameters TMRR 75% and TMRR 5 (tumour-to-muscle retention ratio including voxels of 75% or more of the maximum radioactivity in a VOI and the five hottest pixels, respectively) also served as measures for quantifying the heterogeneous [

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“…The postulated assumption that [ 18 F]FMISO traces viable hypoxic cells is supported by various studies [18,43,46].…”

Section: Discussionmentioning

confidence: 88%

NanoPET imaging of [18F]fluoromisonidazole uptake in experimental mouse tumours

Wyss

Honer

Schubiger

et al. 2005

Eur J Nucl Med Mol Imaging

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“…Bentzen et al (2002) found no correlation between [ 18 F]FMISO-PET and pO 2 electrode measurements in C3H mammary carcinomas. Piert et al (1999Piert et al ( , 2000 showed a correlation between [ 18 F]FMISO-PET data and pO 2 electrode measurements in a study of hypoxia in pig liver tissue. Until today, however, the potential of this PET technique still needs confirmation by appropriate procedures, such as comparative evaluation with nitroimidazolerelated assays.…”

Section: Discussionmentioning

confidence: 93%

Evaluation of hypoxia in an experimental rat tumour model by [18F]Fluoromisonidazole PET and immunohistochemistry

et al. 2004

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This study aimed to evaluate tumour hypoxia by comparing [ 18 F]Fluoromisonidazole uptake measured using positron emission tomography ([ 18 F]FMISO-PET) with immunohistochemical (IHC) staining techniques. Syngeneic rhabdomyosarcoma (R1) tumour pieces were transplanted subcutaneously in the flanks of WAG/Rij rats. Tumours were analysed at volumes between 0.9 and 7.3 cm 3 . Hypoxic volumes were defined using a 3D region of interest on 2 h postinjection [ 18 F]FMISO-PET images, applying different thresholds (1.2 -3.0). Monoclonal antibodies to pimonidazole (PIMO) and carbonic anhydrase IX (CA IX), exogenous and endogenous markers of hypoxia, respectively, were used for IHC staining. Marker-positive fractions were microscopically measured for each tumour, and hypoxic volumes were calculated. A heterogeneous distribution of hypoxia was observed both with histology and

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“…3 and 4). In the work by Piert et al (26,40), it was found that, regardless of ventilation conditions, a signicant inverse relationship existed between mean pO 2 measurements by the Eppendorf electrode and the standardized uptake value of [ 18 F ]F M ISO evaluated by PET examination in liver tissue. The authors concluded that [ 18 F ]F M ISO PET allowed for in vivo quanti cation of pig liver hypoxia.…”

Section: Hypoxia Measurements and Volumementioning

confidence: 98%