Dependency on Acid-base Status of Oxyhemoglobin Dissociation and 2, 3-Diphosphoglycerate Level in Human Erythrocytes: II. In Vivo Studies

Astrup, Poul; Rörth, M.; Thorshauge, C

doi:10.3109/00365517009049213

Cited by 68 publications

(17 citation statements)

References 12 publications

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“…Descriptive studies in man (28,29) and animals (30) have indicated that an initial respiratory alkalosis in response to altitude hypoxia precedes a later elevation in red cell 2,3-DPG. A strong association of blood pH and red cell 2,3-DPG has also been shown in analytical studies of patients with changes in acid-base balance (25,(31)(32)(33)(34)(35)(36). In addition, experimental studies in man have provided evidence of the causal dependence of red cell 2,3-DPG content on blood pH (22,25,32).…”

Section: Methodsmentioning

confidence: 87%

“…A strong association of blood pH and red cell 2,3-DPG has also been shown in analytical studies of patients with changes in acid-base balance (25,(31)(32)(33)(34)(35)(36). In addition, experimental studies in man have provided evidence of the causal dependence of red cell 2,3-DPG content on blood pH (22,25,32). The possible role of elevated pH in modulating rates of enzyme activity leading to the heightened cellular 2,3-DPG levels have been discussed in detail previously (2,30,37).…”

Section: Methodsmentioning

confidence: 99%

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The Relationships between Arterial Oxygen Flow Rate, Oxygen Binding by Hemoglobin, and Oxygen Utilization after Myocardial Infarction

Lichtman¹,

Cohen²,

Young³

et al. 1974

J. Clin. Invest.

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A B S T R A C T The interrelationships of arterial oxygen flow rate index, oxygen binding by hemoglobin, and oxygen consumption have been examined in patients with acute myocardial infarction. Proportional extraction of oxygen increased in close association with decreasing oxygen flow rate, and hence, whole body oxygen consumption was constant over nearly a threefold variation in arterial oxygen flow rate. A reduction in hemoglobin-oxygen affinity at in vivo conditions of pH, PCo2 and temperature also occurred in proportion to the reduction in arterial oxygen flow rate. Therefore, the increased proportional removal of oxygen from arterial blood at low oxygen flow rates, required to maintain oxygen consumption, may have been facilitated by the reduced affinity of hemoglobin for oxygen at in vivo conditions. However, the decrease in affinity did not appear to explain more than 30-40%0 of the increased extraction.Respiratory alkalosis was a frequent occurrence in these patients and 2,3-diphosphoglycerate was positively associated with blood pH as well as with the time-averaged proportion of deoxyhemoglobin in arterial and venous blood.Hemoglobin-oxygen affinity measured at standard conditions and the mixed venous oxygen saturation were equally good indicators of reduced arterial oxygen flow rate in patients without shock. However, Svro2 is more easily measured and is a more useful indicator of reduced oxygen flow rate, since its relationship to oxygen flow appears to be independent of affinity changes and time.

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Section: Methodsmentioning

confidence: 87%

Section: Methodsmentioning

confidence: 99%

The Relationships between Arterial Oxygen Flow Rate, Oxygen Binding by Hemoglobin, and Oxygen Utilization after Myocardial Infarction

Lichtman¹,

Cohen²,

Young³

et al. 1974

J. Clin. Invest.

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“…2,3-DPG was also highly correlated with pHi ( fig. 3) as shown already by Astrup et al [1]. However, when intermediary values -i.e.…”

Section: Discussionmentioning

confidence: 68%

Exchange Transfusion in the Neonate with ACD or CPD Stored Blood

Bursaux¹,

Fréminet²,

Brossard³

et al. 1973

Neonatology

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The blood of two groups of five neonates was exchanged to manage a hemolysis due to ABO incompatibility. Exchange transfusions were performed with ACD or CPD blood stored for less than 48 h. Blood oxygen affinity, hemoglobin, and 2,3-diphosphoglycerate concentrations, plasma and erythrolysate pH were measured on blood samples before and several times during the 24 h following the exchange. Infants transfused with CPD blood increased their P50 and 2,3-DPG concentration more rapidly than the group receiving ACD blood. Estimates of oxygen unloading capacities have been calculated, taking into account variations in hemoglobin concentration, P₅₀, and pH. Assuming a constant cardiac output, transfusion with CPD blood might be more valuable than with ACD blood to insure oxygen transport, especially in infants with respiratory or cardiac impairments.

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“…According to results obtained by other investigators [1,11,18], the oxygen affinity of the hemoglobin in adult patients with metabolic acid-base disorders is much more dependent on the 2,3-DPG content in the erythro cytes. In in vitro studies on reduced and oxygenated blood, Rörth [19] has shown that this effect, counteracting the pH effect, leads to a nearly constant oxygen affinity at low pH values.…”

Section: Discussionmentioning

confidence: 76%

“…A st r u p et at. [1] have shown that a 0.01-pH change of plasma pH within the range of pH 7.30-7.50 will change the 2,3-DPG concentration in the erythro cytes as approximately 0.04 mole 2.3-DPG/mole hemoglobin tetramer.…”

mentioning

confidence: 99%

Bohr Effect, 2,3-DPG and ATP Concentrations in the Blood of Premature Infants with RDS

Manzke¹,

Dörner²

1973

Neonatology

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In children with RDS, the concomitant respiratory-metabolic acidosis leads with the Bohr effect to a lowering of oxygen affinity (increases of P₅₀).However, pH reduction and resultant lowering of 2,3-DPG concentration in the erythrocytes make one expect an oxygen affinity increase. The question is thus to what extent the two influences overlap and neutralize each other. We wanted to take a look at this problem as part of a more extensive study, and so we measured the acid-base status, the oxygen affinity of hemoglobin, the 2,3-DPG, ATP, ADP and AMP contents in the blood of premature infants with RDS and in some term infants with RDS; then we compared these measurements with those of premature infants without RDS.

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Dependency on Acid-base Status of Oxyhemoglobin Dissociation and 2, 3-Diphosphoglycerate Level in Human Erythrocytes: II. In Vivo Studies

Cited by 68 publications

References 12 publications

The Relationships between Arterial Oxygen Flow Rate, Oxygen Binding by Hemoglobin, and Oxygen Utilization after Myocardial Infarction

The Relationships between Arterial Oxygen Flow Rate, Oxygen Binding by Hemoglobin, and Oxygen Utilization after Myocardial Infarction

Exchange Transfusion in the Neonate with ACD or CPD Stored Blood

Bohr Effect, 2,3-DPG and ATP Concentrations in the Blood of Premature Infants with RDS

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