Dephytinization of food stuffs by phytase of <i>Geobacillus</i> sp. TF16 immobilized in chitosan and calcium-alginate

Şirin, Yakup; Akatin, Melike Yildirim; Çolak, Ahmėt; Ertunga, Nagihan Saglam

doi:10.1080/10942912.2016.1261151

Cited by 14 publications

(4 citation statements)

References 46 publications

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“…The resulting capsules were dried between two sheets of filter paper and in open air for 2 h prior to use. The binding percentage was calculated by subtracting the remaining enzyme content in the filtrate solution from the initial enzyme concentration . The immobilization yield was determined by using eq . I m m o b i l i z a t i o n .25em y i e l d ( % ) = ( normalm normalg normalo normalf normalb normalo normalu normaln normald normali normaln normalv normale normalr normalt normala normals normale / normalm normalg normalo normalf normali normaln normali normalt normali normala normall normali normaln normalv normale normalr normalt normala normals normale ) × 100 …”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…The binding percentage was calculated by subtracting the remaining enzyme content in the filtrate solution from the initial enzyme concentration. 29 The immobilization yield was determined by using eq 1 . …”

Section: Methodsmentioning

confidence: 99%

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Industrial Approach to Invertase Production from Fruit Waste for Enhanced Efficiency and Conservation

Dokuzparmak

2024

ACS Omega

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This study investigates the commercial viability of repurposing fruit waste for enzyme production, specifically focusing on the invertase enzyme derived from Saccharomyces cerevisiae. By utilizing fruit pulp that incorporates mulberry, carob, Figure, and grape pulp as a nutrient source, it is observed that the culture medium containing carob pulp exhibits the highest invertase activity. Specifically, the invertase activity in this medium is approximately 2.5 times greater (12.90 U/mg protein) than that observed in the peptone medium (5.98 U/mg protein). The extract undergoes several purification steps, including ultrafiltration, ammonium sulfate precipitation, dialysis, and ion-exchange chromatography (purification ratio: 12.11 times, yield: 26.93%). The purified enzyme is immobilized using alginate beads, improving pH and thermal stability. The immobilized enzyme exhibits optimal activity between pH 3.50 and pH 7.00, thereby broadening the enzyme's high-activity pH range. The thermal stability of the immobilized invertase enzyme is significantly improved, especially at 65 °C. Activity studies in the presence of metal ions and certain chemicals have been conducted. The immobilized enzyme's activity increases by approximately 40% in the presence of Ca 2+ and Mg 2+ , and the immobilized enzyme maintains its activity in the presence of detergents such as SDS, Tween-20, and organic solvents like ethanol and methanol. The potential for the reuse of immobilized invertase was investigated under standard assay conditions. After 20 cycles, the immobilized enzyme was found to retain 80% of its initial activity. Overall, the study establishes the commercial potential of fruit pulp, typically discarded in fruit juice production, as a valuable source for obtaining an invertase enzyme. Furthermore, this study also aims to develop a suitable purification process for invertase in the fruit juice industry. By harnessing fruit waste and implementing innovative enzyme production strategies, industries can enhance their efficiency, reduce their environmental footprint, and optimize resource utilization.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Industrial Approach to Invertase Production from Fruit Waste for Enhanced Efficiency and Conservation

Dokuzparmak

2024

ACS Omega

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“…TF-16 for soy milk phytate was calculated as 24 and 33%, respectively. The chitosan-and Ca-alginate immobilized enzymes conserved their original activity after 8 and 6 cycles of reuse, respectively [114].…”

Section: Production and Applications Of Thermostable Enzymes Of Geobamentioning

confidence: 99%

GeobacillusBacteria: Potential Commercial Applications in Industry, Bioremediation, and Bioenergy Production

Новик¹,

Savich²,

Meerovskaya³

2019

Growing and Handling of Bacterial Cultures

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The genus Geobacillus is represented by obligately thermophilic bacteria able to grow in the temperature range of 35-75°C. They are modest bacteria isolated from various sources on routine media such as nutrient agar. Originally classified as representatives of Bacillus, the species of Geobacillus were established in 2001 as a new genus. However, sequence similarity between all species indicates that at least some species need to be reclassified at the genus level. In addition to 16S rRNA, housekeeping genes, 16S-23S rRNA gene internal transcribed spacer, and repetitive sequences can be used in classification and identification of thermophilic bacteria. The ability to survive and grow at high temperatures as well as utilization and synthesis of a wide range of compounds makes these bacteria and their products attractive for use in various spheres: food, paper, biotechnology industries, medicine, bioremediation, etc. A broad spectrum of applications arouses increased interest in the study of physiological and biochemical characteristics and triggers emergence of new usage areas for Geobacillus, such as bioenergy. The growing demand for energy leads to the development of alternative technologic options. Geobacillus species demonstrated the ability to generate or enhance productivity of important sources of bioenergy such as ethanol, isobutanol, 2,3-butanediol, biodiesel, and biogas.

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Thermophilic and Halophilic Microorganisms Isolated from Extreme Environments of Turkey, with Potential Biotechnological Applications

Güven

Bekler

Güven

2018

Extremophiles in Eurasian Ecosystems: Ecology, Diversity, and Applications

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Dephytinization of food stuffs by phytase of Geobacillus sp. TF16 immobilized in chitosan and calcium-alginate

Cited by 14 publications

References 46 publications

Industrial Approach to Invertase Production from Fruit Waste for Enhanced Efficiency and Conservation

Industrial Approach to Invertase Production from Fruit Waste for Enhanced Efficiency and Conservation

GeobacillusBacteria: Potential Commercial Applications in Industry, Bioremediation, and Bioenergy Production

Thermophilic and Halophilic Microorganisms Isolated from Extreme Environments of Turkey, with Potential Biotechnological Applications

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