Thermophilic and Halophilic Microorganisms Isolated from Extreme Environments of Turkey, with Potential Biotechnological Applications

Güven, Kemal; Bekler, Fatma Matpan; Güven, Reyhan Gül

doi:10.1007/978-981-13-0329-6_8

Cited by 7 publications

(6 citation statements)

References 91 publications

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“…Turkey is one of the world's most significant countries because of its abundant thermal water reserves (Oztas Gulmus and Gormez 2020b ). Hot springs in eastern and southeastern Turkey are reservoirs for natural resources that are attractive for biotechnological studies (Guven et al 2018 ). Various thermophilic microbial species have been reported in many studies carried out in the Hasanabdal thermal springs (Poli et al 2012 ; Savas et al 2009 ; Yanmis et al 2015 ), Sirnak (Derya and Ahmet 2014 ), Diyadin (Baltaci et al 2017 ), Dargecit (Acer et al 2015 ), Guclukonak (Poli et al 2012 ) and Pasinler (Adiguzel et al 2011 ; Oztas Gulmus and Gormez 2020b ).…”

Section: Introductionmentioning

confidence: 99%

Identification, characterization and hydrolase producing performance of thermophilic bacteria: geothermal hot springs in the Eastern and Southeastern Anatolia Regions of Turkey

Uluçay

Görmez

Öziç

2022

Antonie van Leeuwenhoek

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In the last two decades, researchers have increasingly focused on the rich microorganism-based diversity of natural hot spring sources to explore the benefits of thermophiles in industrial and biotechnological fields. Within the scope of this study, a total of 83 thermophilic Bacilli strains were isolated from 7 different geothermal hot springs (at temperatures ranging between 40 and 85 °C) located in the Eastern and Southeastern Anatolia Regions of Turkey. The physiological, morphological, biochemical and molecular properties of the isolates were determined. As a result of the 16S rRNA gene sequence analysis, 5 different species ( Bacillus licheniformis , Bacillus sp . , Bacillus subtilis , Geobacillus kaustophilus, and Weizmannia coagulans ,) were identified. B. licheniformis and B. subtilis were the most frequently encountered species among those obtained from the researched hot spring sources. Phylogenetic analysis was conducted to evaluate the phylogenetic relationships of the isolated species. The results showed that there was no significant difference between the groups and the bacteria in terms of the locations or optimum temperatures of the isolates. The bacterial isolates were screened for amylase, cellulase, lipase and protease hydrolytic enzyme activities. The hydrolytic enzyme production potentials among the isolates were identified in 68 (82%) isolates for amylase, 34 (41%) for cellulase, 69 (83%) for lipase and 73 (88%) for protease. All isolates were found to have at least one or more extracellular enzyme activities. Additionally, it was determined that 27 of the existing isolates (32.8%) were able to produce all of the aforementioned hydrolytic enzymes. Supplementary Information The online version contains supplementary material available at 10.1007/s10482-021-01678-5.

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Section: Introductionmentioning

confidence: 99%

Identification, characterization and hydrolase producing performance of thermophilic bacteria: geothermal hot springs in the Eastern and Southeastern Anatolia Regions of Turkey

Uluçay

Görmez

Öziç

2022

Antonie van Leeuwenhoek

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“…is described for the use in a biological RPB treatment process. The discovery and usage of novel isolated halophilic microorganisms and their exploitation in a biotechnological and industrial context was already successfully demonstrated ( Guven et al, 2018 ; Rezaei Somee et al, 2018 ; Zare et al, 2019 ; Hasanzadeh et al, 2020 ; Villanova et al, 2021 ). Besides, the usage of a co-culture might be even beneficial for the present bioprocess.…”

Section: Resultsmentioning

confidence: 99%

Reducing Organic Load From Industrial Residual Process Brine With a Novel Halophilic Mixed Culture: Scale-Up and Long-Term Piloting of an Integrated Bioprocess

Mainka

Herwig

Pflügl

2022

Front. Bioeng. Biotechnol.

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Integrating bioprocess solutions for treatment and subsequent reuse of saline residual process brine into industrial processes could increase the sustainability of production chains. However, such bioprocesses require large-scales and a robust operation over a prolonged period. Consequently, the aim of this study was to analyze scale-up equivalence as well as continuous and stable process performance of a previously established lab scale process for the degradation of organic contaminants (formate and aromatic compounds) in an industrial context. To that end, a pilot-scale bubble column bioreactor system equipped with a membrane-based cell retention system for process intensification was integrated at an industrial production site. The process was successfully scaled-up and continuously operated for more than 210 days. Overall, the process proved to be robust towards changing compositions of the residual process brine stream and degradation rates for organic contaminants were close to 100%. Interestingly, due to the unsterile process conditions, the original Haloferax mediterranei culture was replaced by a novel halophilic bacterial community consisting of three bacterial genera. To further improve process economics and productivity, an optimization of the co-substrate feeding strategy for glycerol is required, as results indicated a potential correlation between glycerol feeding and formate degradation rates. To that end, decoupling of the glycerol feeding from the residual process brine feed is a potential way to increase process control options and allow for easy adaptation of the process to changing residual process brine compositions. Ultimately, the process described here could be a promising alternative for chemical or physical methods of treating residual process brine and once more underlines the potential to exploit natural microbial diversity for industrial purposes.

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“…There is well-known basic work on the genetic diversity and structure of microbial communities in salt lakes, in which halophilic microorganisms (including bacteria and archaea) isolated and identified from the salt lakes and their sediments can be regarded as an important part of biological community ( Guven et al, 2018 ; Selivanova et al, 2018 ). At present, most of the sampling points in these studies are from estuaries, salt ponds, and sediments of salt lakes ( Wu et al, 2006 ; Boujelben et al, 2012 ; Liu et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Exploring Microbial Resource of Different Rhizocompartments of Dominant Plants Along the Salinity Gradient Around the Hypersaline Lake Ejinur

et al. 2021

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Lake littoral zones can also be regarded as another extremely hypersaline environment due to hypersaline properties of salt lakes. In this study, high-throughput sequencing technique was used to analyze bacteria and fungi from different rhizocompartments (rhizosphere and endosphere) of four dominant plants along the salinity gradient in the littoral zones of Ejinur Salt Lake. The study found that microbial α-diversity did not increase with the decrease of salinity, indicating that salinity was not the main factor on the effect of microbial diversity. Distance-based redundancy analysis and regression analysis were used to further reveal the relationship between microorganisms from different rhizocompartments and plant species and soil physicochemical properties. Bacteria and fungi in the rhizosphere and endosphere were the most significantly affected by SO42–, SOC, HCO3–, and SOC, respectively. Correlation network analysis revealed the potential role of microorganisms in different root compartments on the regulation of salt stress through synergistic and antagonistic interactions. LEfSe analysis further indicated that dominant microbial taxa in different rhizocompartments had a positive response to plants, such as Marinobacter, Palleronia, Arthrobacter, and Penicillium. This study was of great significance and practical value for understanding salt environments around salt lakes to excavate the potential microbial resources.

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Thermophilic and Halophilic Microorganisms Isolated from Extreme Environments of Turkey, with Potential Biotechnological Applications

Cited by 7 publications

References 91 publications

Identification, characterization and hydrolase producing performance of thermophilic bacteria: geothermal hot springs in the Eastern and Southeastern Anatolia Regions of Turkey

Identification, characterization and hydrolase producing performance of thermophilic bacteria: geothermal hot springs in the Eastern and Southeastern Anatolia Regions of Turkey

Reducing Organic Load From Industrial Residual Process Brine With a Novel Halophilic Mixed Culture: Scale-Up and Long-Term Piloting of an Integrated Bioprocess

Exploring Microbial Resource of Different Rhizocompartments of Dominant Plants Along the Salinity Gradient Around the Hypersaline Lake Ejinur

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