Dépistage et typage des infections à HPV par technique INNO-LiPA sur milieu liquide Easyfix Labonord après extraction QIAamp DNA Blood Mini Kit® Qiagen et Nuclisens easyMAG® Biomérieux

Hantz, Sébastien; Goudard, M.; Marczuk, V.; Renaudie, J.; Dussartre, C.; Bakeland, D.; Denis, F.; Alain, Sophie

doi:10.1016/j.patbio.2009.07.019

Cited by 4 publications

(3 citation statements)

References 16 publications

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“…Materials from swab specimens were suspended in 3mL of phosphate buffered saline (PBS). DNA was extracted from 750μl using the EasyMAG NucliSens extraction platform (BioMérieux, Boxtel, the Netherlands) [ 14 ]. The extracted DNA was eluted in 100μl of wash buffer 3.…”

Section: Methodsmentioning

confidence: 99%

Randomized trial evaluating self-sampling for HPV DNA based tests for cervical cancer screening in Nigeria

Modibbo

Iregbu

Okuma

et al. 2017

Infect Agents Cancer

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BackgroundCervical cancer incidence and mortality rates in Sub-Saharan Africa (SSA) remain high due to several factors including low levels of uptake of cervical cancer screening. Self-collection of cervicovaginal samples for HPV DNA testing may be an effective modality that can increase uptake of cervical cancer screening in SSA and hard to reach populations in developed countries. We investigated whether self-collection of cervicovaginal samples for HPV DNA tests would be associated with increased uptake of screening compared with clinic based collection of samples. Furthermore, we compared the quality of samples collected by both approaches for use in HPV genotyping.MethodsWe conducted a community based randomized trial in a semi-urban district of Abuja, Nigeria with 400 women, aged 30 to 65 years randomized to either hospital-collection or self-collection of cervicovaginal samples. We compared cervical cancer screening uptake among the 2 groups and evaluated the concentration of human DNA in the samples by measuring RNase P gene levels using qPCR. High-risk HPV DNA detection and typing was done using the GP5+/6+ Luminex system.ResultsMost participants in the self-collection arm (93%, 185/200) submitted their samples while only 56% (113/200) of those invited to the hospital for sample collection attended and were screened during the study period (p value < 0.001). Human genomic DNA was detected in all but five (1.7%) participants, all of whom were in the self-collection arm. The prevalence of high-risk HPV in the study population was 10% with types 35, 52 and 18 being the commonest.ConclusionsOur study shows that self-sampling significantly increased uptake of HPV DNA based test for cervical cancer screening in this population and the samples collected were adequate for HPV detection and genotyping. Cervical cancer screening programs that incorporate self-sampling and HPV DNA tests are feasible and may significantly improve uptake of cervical cancer screening in SSA.

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Section: Methodsmentioning

confidence: 99%

Randomized trial evaluating self-sampling for HPV DNA based tests for cervical cancer screening in Nigeria

Modibbo

Iregbu

Okuma

et al. 2017

Infect Agents Cancer

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“…DNA was isolated from peripheral blood samples by the same standard techniques for all volunteers, as previously described (21). The integrity of DNA in every sample was determined by DNA agarose gel electrophoresis, as previously described (22), and the concentration of DNA in every sample was detected by a Qubit 2.0 fluorometer (Thermo Fisher Scientific, Inc., Waltham, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

Whole-genome resequencing of 100 healthy individuals using DNA pooling

Wang¹,

Sui²,

Wu³

et al. 2016

Experimental and Therapeutic Medicine

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With the advent of next-generation sequencing technology, the cost of sequencing has significantly decreased. However, sequencing costs remain high for large-scale studies. In the present study, DNA pooling was applied as a cost-effective strategy for sequencing. The sequencing results for 100 healthy individuals obtained via whole-genome resequencing and using DNA pooling are presented in the present study. In order to minimise the likelihood of systematic bias in sampling, paired-end libraries with an insert size of 500 bp were prepared for all samples and then subjected to whole-genome sequencing using four lanes for each library and resulting in at least a 30-fold haploid coverage for each sample. The NCBI human genome build37 (hg19) was used as a reference genome for the present study and the short reads were aligned to the reference genome achieving 99.84% coverage. In addition, the average sequencing depth was 32.76. In total, ~3 million single-nucleotide polymorphisms were identified, of which 99.88% were in the NCBI dbSNP database. Furthermore, ~600,000 small insertion/deletions, 500,000 structure variants, 5,000 copy number variations and 13,000 single nucleotide variants were identified. According to the present study, the whole genome has been sequenced for a small sample subjects from southern China for the first time. Furthermore, new variation sites were identified by comparing with the reference sequence, and new knowledge of the human genome variation was added to the human genomic databases. Furthermore, the particular distribution regions of variation were illustrated by analyzing various sites of variation, such as single-nucleotide polymorphisms.

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“…Until now, only the Specimen Transport Medium (STM) (Qiagen), the PreservCyt Solution (Cytyc/Hologic) and the BD SurePath Pap Test (BD) are recommended to collect cervical specimens for HPV testing with the Hybrid Capture 2 (HC2) assay and specific studies have been conducted to validate the suitability of different LBC media. [10][11][12][13][14][15] The Novaprep ® Vial Test (NVT) is a new device dedicated to LBC. Following cervical sampling, the cytobrush head is plunged and kept in a vial containing an alcohol/formalin-based fixative.…”

Section: Introductionmentioning

confidence: 99%

Novaprep® Vial Test is a suitable liquid-based cytology medium for high risk human papillomavirus testing by Hybrid Capture 2

Prétet¹,

Vidal²,

Carval³

et al. 2010

Journal of Clinical Virology

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Dépistage et typage des infections à HPV par technique INNO-LiPA sur milieu liquide Easyfix Labonord après extraction QIAamp DNA Blood Mini Kit® Qiagen et Nuclisens easyMAG® Biomérieux

Cited by 4 publications

References 16 publications

Randomized trial evaluating self-sampling for HPV DNA based tests for cervical cancer screening in Nigeria

Randomized trial evaluating self-sampling for HPV DNA based tests for cervical cancer screening in Nigeria

Whole-genome resequencing of 100 healthy individuals using DNA pooling

Novaprep® Vial Test is a suitable liquid-based cytology medium for high risk human papillomavirus testing by Hybrid Capture 2

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