Depletion of dietary phytoestrogens reduces hippocampal plasticity and contextual fear memory stability in adult male mouse

Çalışkan, Gürsel; Raza, Syed Ahsan; Demiray, Yunus Emre; Kul, Emre; Sandhu, Kawaljit Singh; Stork, Oliver

doi:10.1080/1028415x.2019.1698826

Cited by 11 publications

(10 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PEs functionally and structurally mimic mammalian estrogens and their active metabolites, which in turn can modulate estrogen-sensitive pathways (Mäkelä et al, 1995). In one study, animals fed a diet lacking PEs experienced deficits in learning and memory, which was rescued when subjects were supplied exogenous equol (a metabolite of estrogen; (Çalişkan et al, 2019). In contrast, animals fed a diet high in PEs experienced profound alterations in energy balance including reduced body weight, adiposity, and increased lipid oxidation (Cederroth et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

The Development, but not Expression, of Alcohol Front-loading in C57BL/6J Mice Maintained on LabDiet 5001 is Abolished by Maintenance on Teklad 2920x Rodent Diet

Maphis

Huffman

Linsenbardt

2022

Preprint

View full text Add to dashboard Cite

Excessive alcohol (ethanol) consumption, such as binge-drinking, is extremely commonplace and represents a major health concern. Through modeling excessive drinking in rodents, we are beginning to uncover the neurobiological and neurobehavioral causes and consequences of this pattern of ethanol intake. One important factor for modeling binge drinking in mice is that subjects reliably drink to blood ethanol concentrations (BECs) of 80 mg/dl or higher. Drinking-in-the-dark (DID) is a commonly used mouse model of binge drinking, and we have shown these methods reliably result in robust ethanol front-loading and binge-level BECs in C57BL/6J (B6) mice as well as other ethanol-preferring mouse strains/lines. However, establishing the DID model in a new vivarium space forced us to consider the use of rodent diet formulations we had not previously used. The current set of experiments were designed to investigate the role of two standard rodent diet formulations on binge drinking and the development of ethanol front-loading using DID. We found that BECs in animals maintained on LabDiet 5001 (LD01) were double those found in mice maintained on Teklad 2920x (TL20). Interestingly, this effect was paralleled by differences in the degree of front-loading, such that LD01-fed mice consumed approximately twice as much ethanol in the first 15 minutes of the 2-hour DID sessions compared to TL20-fed mice. Surprisingly however, mice that developed front-loading during maintenance on the LD01 diet continued to display front-loading behavior after being switched to the TL20 diet. These data emphasize the importance of choosing and reporting diet formulations when conducting voluntary drinking studies and support the need for further investigation into the mechanisms behind diet-induced differences in binge drinking, particularly front-loading.

show abstract

Section: Discussionmentioning

confidence: 99%

The Development, but not Expression, of Alcohol Front-loading in C57BL/6J Mice Maintained on LabDiet 5001 is Abolished by Maintenance on Teklad 2920x Rodent Diet

Maphis

Huffman

Linsenbardt

2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Accordingly, a recent work (Chu et al, 2019) shows that Abx-treated mice show impaired extinction of a cued fear memory. Both of these fear paradigms are dependent on the functional interactions between the hippocampus and amygdala (Çalışkan et al, 2019) and amygdalar activity appears to be recruited during SW events (Girardeau et al, 2017). Thus, increased SW incidence might partially predispose for re-consolidation of original fear memories and impair their extinction in Abx-treated mice.…”

Section: Discussionmentioning

confidence: 99%

“…Slice electrophysiology was performed as described previously (Caliskan et al, 2015; Çalışkan et al, 2019). After 6 weeks of chronic antibiotic treatment, decapitation of mice and brain extraction were performed under deep isoflurane anesthesia.…”

Section: Methodsmentioning

confidence: 99%

Antibiotic treatment induces activation of microglia and impairment of cholinergic gamma oscillations in the hippocampus

Çalışkan

French

Heimesaat

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Antibiotics are widely applied for the treatment of bacterial infections, but their long-term use may lead to gut flora dysbiosis and detrimental effects on brain physiology, behavior as well as cognitive performance. Still, a striking lack of knowledge exists concerning electrophysiological correlates of antibiotic-induced changes in gut microbiota and behavior. Here, we investigated changes in the synaptic transmission and plasticity together with behaviorally-relevant network activities from the hippocampus of antibiotic-treated mice. The prolonged antibiotic treatment led to a strong reduction of myeloid cell pools in bone marrow, circulation and those surveilling the brain. Upon antibiotic treatment, circulating Ly6Chi inflammatory monocytes adopted a proinflammatory phenotype with increased expression of CD40 and MHC II. In the central nervous system, microglia displayed typical signs of activation with elevated CD40 and MHC II expression, as well as increased IL-6 and TNF production. Concomitantly, we detected a substantial reduction in the synaptic transmission in the hippocampal CA1 after antibiotic treatment. In line, carbachol-induced cholinergic gamma oscillation were reduced upon antibiotic treatment while the incidence of hippocampal sharp waves was elevated. These changes were associated with the global changes in the expression of neurotrophin nerve growth factor and inducible nitric oxide synthase, both of which have been shown to influence cholinergic functions. Overall, our study demonstrates that antibiotic-induced changes in the gut microbiome and immune cell function are associated with a drastic reduction in the synaptic transmission and gamma oscillations in the hippocampus, a brain region that is critically involved in mediation of innate and cognitive behavior.

show abstract

“…This diet induced a profound decrease in long-term potentiation (LTP) in the ventral hippocampus, altered territorial marking behavior, a reduction of intermale aggression, and a general disturbance of social behavior. Additionally, acute perfusion of equol was able to rescue this LTP deficit, demonstrating a possible modulation by phytoestrogen of the hippocampus plasticity, as well as memory function [ 55 ].…”

Section: Neuroprotective Effects Of Selected Phytoestrogensmentioning

confidence: 99%

The Potential Effects of Phytoestrogens: The Role in Neuroprotection

2021

View full text Add to dashboard Cite

Phytoestrogens are naturally occurring non-steroidal phenolic plant compounds. Their structure is similar to 17-β-estradiol, the main female sex hormone. This review offers a concise summary of the current literature on several potential health benefits of phytoestrogens, mainly their neuroprotective effect. Phytoestrogens lower the risk of menopausal symptoms and osteoporosis, as well as cardiovascular disease. They also reduce the risk of brain disease. The effects of phytoestrogens and their derivatives on cancer are mainly due to the inhibition of estrogen synthesis and metabolism, leading to antiangiogenic, antimetastatic, and epigenetic effects. The brain controls the secretion of estrogen (hypothalamus-pituitary-gonads axis). However, it has not been unequivocally established whether estrogen therapy has a neuroprotective effect on brain function. The neuroprotective effects of phytoestrogens seem to be related to both their antioxidant properties and interaction with the estrogen receptor. The possible effects of phytoestrogens on the thyroid cause some concern; nevertheless, generally, no serious side effects have been reported, and these compounds can be recommended as health-promoting food components or supplements.

show abstract

Depletion of dietary phytoestrogens reduces hippocampal plasticity and contextual fear memory stability in adult male mouse

Cited by 11 publications

References 72 publications

The Development, but not Expression, of Alcohol Front-loading in C57BL/6J Mice Maintained on LabDiet 5001 is Abolished by Maintenance on Teklad 2920x Rodent Diet

The Development, but not Expression, of Alcohol Front-loading in C57BL/6J Mice Maintained on LabDiet 5001 is Abolished by Maintenance on Teklad 2920x Rodent Diet

Antibiotic treatment induces activation of microglia and impairment of cholinergic gamma oscillations in the hippocampus

The Potential Effects of Phytoestrogens: The Role in Neuroprotection

Contact Info

Product

Resources

About