Deposition of DNA Nanostructures on Highly Oriented Pyrolytic Graphite

Abstract

We report the deposition of DNA origami nanostructures on highly oriented pyrolytic graphite (HOPG). The DNA origami goes through a structural rearrangement and the DNA base is exposed to interact with the graphite surface. Exposure to ambient air, which is known to result in a hydrophilic-to-hydrophobic wetting transition of HOPG, does not significantly impact the deposition yield or the shape deformation of DNA nanostructures. The deposited DNA nanostructures maintain their morphology for at least a week and promote site-selective chemical vapor deposition of SiO. This process is potentially useful for a range of applications that include but are not limited to nanostructure fabrication, sensing, and electronic and surface engineering.

DNA‐Based Strategies for Site‐Specific Doping

“…We and others have reported protocols to achieve high resolution pattern transfer (10 nm resolution demonstrated in some cases) from DNA templates to SiO 2 , Si, graphene, polymer, and SAMs. [19][20][21][22][23][24][25][26] Here, we show the programmability Site-specific doping is an essential component in the manufacturing of almost all semiconductor devices, such as transistors, [1,2] sensors, [3] and photovoltaics. [4] The doping type (n or p), concentration, and spatial profile of the dopant atoms impact many, if not all, operation characteristics of the device.…”

“…We and others have reported protocols to achieve high resolution pattern transfer (10 nm resolution demonstrated in some cases) from DNA templates to SiO 2 , Si, graphene, polymer, and SAMs. [ 19–26 ] Here, we show the programmability of the DNA nanostructure also enables site‐specific doping of Si wafer.…”

Revealing DNA Structure at Liquid/Solid Interfaces by AFM-Based High-Resolution Imaging and Molecular Spectroscopy

“…High-resolution AFM imaging requires surface fixation of studied analyte. So far, various surfaces have been used for DNA imaging, for example, cationic lipid bilayers [5], gold [6,7], highly oriented pyrolytic graphite (HOPG) [8][9][10][11][12][13][14][15], and mica [16][17][18][19]. Due to the atomic flatness, mica provides favorable conditions for achieving a high contrast, which supports obtaining high-resolution AFM images.…”

Area-Selective Atomic Layer Deposition of Metal Oxides on DNA Nanostructures and Its Applications

“…DNA nanostructures also adhere to π systems ( e.g. , highly oriented pyrolytic graphite) via hydrophobic and/or π–π stacking interactions . Inspired by the work of Jin et al , we hypothesized that amphiphilic molecules such as 1-pyrenemethylamine hydrochloride (PMA) could serve as a bridge to anchor DNA nanostructures onto hydrophobic polymer surfaces.…”

“…27 DNA nanostructures also adhere to π systems (e.g., highly oriented pyrolytic graphite) via hydrophobic and/or π−π stacking interactions. 28 Inspired by the work of Jin et al, 29 we hypothesized that amphiphilic molecules such as 1-pyrenemethylamine hydrochloride (PMA) could serve as a bridge to anchor DNA nanostructures onto hydrophobic polymer surfaces. The aromatic basal plane of PMA could interact favorably with hydrophobic polymers such as polystyrene (PS), while its amine group with a positive charge could attract DNA nanostructures via electrostatic interactions.…”