Depot-specific characteristics of adipose tissue-derived stromal cells in thyroid-associated orbitopathy

Wong, Janus Siu Him; Chu, Wai Kit; Li, Benjamin Fuk-Loi; Pang, Chi‐Pui; Chong, Kelvin K L

doi:10.1136/bjophthalmol-2017-311339

Cited by 5 publications

(2 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As to alcian blue staining, the chondrogenic hMSCs were washed with DPBS three times before the addition of Alcian Blue Cartilage Stain Solution, pH = 1.0 (Solarbio, Beijing, China), the cultures were scanned under a phase contrast microscope (NA 0.75, 20×) after 30 min of incubating and three times of washing. As to Nile red fluorescent staining, the adipogenic induced cells were washed three times and stained with 1 µM Nile red (Solarbio, Beijing, China) with Hoechst 33342 (Solarbio, Beijing, China) for 5 min in room temperature [ 21 ]. After three times of washing, a fluorescence microscope (NA 0.9, 40×, Olympus, Tokyo, Japan) was used for scanning and photography.…”

Section: Methodsmentioning

confidence: 99%

Changes in the mechanical properties of human mesenchymal stem cells during differentiation

Yang

Liu

et al. 2023

R. Soc. open sci.

View full text Add to dashboard Cite

A thorough understanding of the changes in mechanical property behind intracellular biophysical and biochemical processes during differentiation of human mesenchymal stem cells (hMSCs) is helpful to direct and enhance the commitment of cells to a particular lineage. In this study, displacement creep of the mesenchymal cell lineages (osteogenic, chondrogenic and adipogenic hMSCs) were determined by using atomic force microscopy, which was then used to determine their mechanical properties. We found that at any stages of differentiation, the mesenchymal cell lineages are linear viscoelastic materials and well matched with a simple power-law creep compliance. In addition, the viscoelasticity of mesenchymal cell lineages showed different trends during differentiation. The adipogenic hMSCs showed continuous softening at all stages. The osteogenic and chondrogenic hMSCs only continuously soften and become more fluid-like in the early stage of differentiation, and get stiffened and less fluid-like in the later stage. These findings will help more accurately imitate cellular biomechanics in the microenvironment, and provided an important reference in the biophysics biomimetic design of stem cell differentiation.

show abstract

Section: Methodsmentioning

confidence: 99%

Changes in the mechanical properties of human mesenchymal stem cells during differentiation

Yang

Liu

et al. 2023

R. Soc. open sci.

View full text Add to dashboard Cite

show abstract

“…Studies have reported that ADSC therapy could be beneficial in thyroid-associated orbitopathy [117]. It has also been suggested that insulin-like growth factor 1 has proadipogenic and pro-proliferative effects on ADSCs extracted from thyroid-associated ophthalmopathy patients via in vitro studies [118].…”

Section: Potential Applications Of Adipose Stem Cells To Periorbital ...mentioning

confidence: 99%

Adipose Stem Cells in Modern-Day Ophthalmology

Musa

Zeppieri

Enaholo³

et al. 2023

Clinics and Practice

View full text Add to dashboard Cite

Stem cells (SCs) have evolved as an interesting and viable factor in ophthalmologic patient care in the past decades. SCs have been classified as either embryonic, mesenchymal, tissue-specific, or induced pluripotent cells. Multiple novel management techniques and clinical trials have been established to date. While available publications are predominantly animal-model-based, significant material is derived from human studies and case-selected scenarios. This possibility of explanting cells from viable tissue to regenerate/repair damaged tissue points to an exciting future of therapeutic options in all fields of medicine, and ophthalmology is surely not left out. Adipose tissue obtained from lipo-aspirates has been shown to produce mesenchymal SCs that are potentially useful in different body parts, including the oculo-visual system. An overview of the anatomy, physiology, and extraction process for adipose-tissue-derived stem cells (ADSC) is important for better understanding the potential therapeutic benefits. This review examines published data on ADSCs in immune-modulatory, therapeutic, and regenerative treatments. We also look at the future of ADSC applications for ophthalmic patient care. The adverse effects of this relatively novel therapy are also discussed.

show abstract

The Relevance and Potential Role of Orbital Fat in Inflammatory Orbital Diseases: Implications for Diagnosis and Treatment

Dadson,

Ngum,

Juarez-Orozco

et al. 2024

Ophthalmol Ther

View full text Add to dashboard Cite

Depot-specific characteristics of adipose tissue-derived stromal cells in thyroid-associated orbitopathy

Cited by 5 publications

References 24 publications

Changes in the mechanical properties of human mesenchymal stem cells during differentiation

Changes in the mechanical properties of human mesenchymal stem cells during differentiation

Adipose Stem Cells in Modern-Day Ophthalmology

The Relevance and Potential Role of Orbital Fat in Inflammatory Orbital Diseases: Implications for Diagnosis and Treatment

Contact Info

Product

Resources

About