Depressed synthesis of DNA in regenerating rat liver after spinal cord (C7) transection

Vaptzarova, K. I.; Popov, P.G.; Veselý, Jiří; Čihák, A.

doi:10.1007/bf01943883

Cited by 10 publications

(3 citation statements)

References 9 publications

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“…Thus 5-HT2 receptors can increase sympathetic nervous discharge [5]. Transection of the spinal cord above the area innervating the liver resulted in decreased DNA synthesis [6]. Altered brain neurotransmission was reported in different liver diseases [7][8][9] and a role for brain serotonin has been suggested in hepatic encephalopathy [10].…”

Section: Introductionmentioning

confidence: 99%

Increased 5-HT2C receptor binding in the brain stem and cerebral cortex during liver regeneration and hepatic neoplasia in rats

Sulaiman

Joseph

Paulose

2007

Journal of the Neurological Sciences

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In the present study, serotonin 2C (5-HT(2C)) receptor binding parameters in the brainstem and cerebral cortex were investigated during liver generation after partial hepatectomy (PH) and N-nitrosodiethylamine (NDEA) induced hepatic neoplasia in male Wistar rats. The serotonin content increased significantly (p<0.01) in the cerebral cortex after PH and in NDEA induced hepatic neoplasia. Brain stem serotonin content increased significantly (p<0.05) after PH and (p<0.001) in NDEA induced hepatic neoplasia. The number and affinity of the 5-HT(2C) receptors in the crude synaptic membrane preparations of the brain stem showed a significant (p<0.001) increase after PH and in NDEA induced hepatic neoplasia. The number and affinity of 5-HT(2C) receptors increased significantly (p<0.001) in NDEA induced hepatic neoplasia in the crude synaptic membrane preparations of the cerebral cortex. There was a significant (p<0.01) increase in plasma norepinephrine in PH and (p<0.001) in NDEA induced hepatic neoplasia, indicating sympathetic stimulation. Thus, our results suggest that during active hepatocyte proliferation 5-HT(2C) receptor in the brain stem and cerebral cortex are up-regulated which in turn induce hepatocyte proliferation mediated through sympathetic stimulation.

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Section: Introductionmentioning

confidence: 99%

Increased 5-HT2C receptor binding in the brain stem and cerebral cortex during liver regeneration and hepatic neoplasia in rats

Sulaiman

Joseph

Paulose

2007

Journal of the Neurological Sciences

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“…In our previous studies we were interested in the effect of different drugs and factors on the incorporation of thymidine into DNA in regenerating rat liver (cihaik & Vesely, 1972;Vaptzarova et al, 1973), and we now describe the marked depression of hepatic DNA synthesis by reserpine. The maximal effect in 24 hour regenerating liver was obtained when the drug was administered before the exponential increase of DNA synthesis, i.e.…”

Section: Resultsmentioning

confidence: 99%

Decreased synthesis of DNA in regenerating rat liver after the administration of reserpine

Čihák

Vaptzarova

1973

British J Pharmacology

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Summary1. Reserpine given to rats before the enhanced synthesis of DNA begins 14h after partial hepatectomy markedly depresses thymidine uptake into DNA at 24 hours. 2. At this time decreased activity of liver thymidine kinase but unchanged thymidine 5'-nucleotidase were observed. 3. Reserpine has no effect on DNA synthesis when administered simultaneously with the labelled thymidine 2 h before killing.4. With depressed DNA synthesis after reserpine administration there is no significant decrease of liver RNA synthesis.

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“…9 Extirpation of the brain cortex was shown to increase the rate of liver cell proliferation describing that the cortex exerts an inhibitory function on liver cell division, growth and also transection of the spinal cord above the area innervating the liver resulted in decreased DNA synthesis. 10 There are several reports highlighting the regulation of hepatic proliferation by the cerebral cortex, but the role of neurotransmitters and their receptors in mediating neuronal survival during neurotransmitter conjugated chitosan nanoparticle induced liver regeneration are not well studied.…”

Section: Introductionmentioning

confidence: 99%

Increased Cortical Neuronal Survival During Liver Injury: Effect of Gamma Aminobutyric Acid and 5-HT Chitosan Nanoparticles

Joy¹,

Anju²,

Ajayan³

et al. 2014

Journal of Biomedical Nanotechnology

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Use of nanoparticulate drug delivery system for an effective therapeutic outcome against diseases gains immense hope in the study of drug delivery to partially hepatectomised rats. In the present study, partially hepatectomised rats treated with Gamma aminobutyric acid (GABA) and serotonin (5-HT) chitosan nanoparticles, individually and in combination, were evaluated to analyse their role in GABAB, and 5-HT2A receptors functional regulation, interrelated neuronal survival mechanisms by nuclear factor kappa B (NF-kappaB), tumour necrosis factor-a (TNF-alpha), Akt-1 and the antioxidant enzyme superoxide dismutase (SOD) in the cerebral cortex. A significant decrease in GABA, and 5-HT2A receptor numbers and gene expressions denoted a homeostatic adjustment by the cerebral cortex to trigger the sympathetic innervations during elevated DNA synthesis in the liver. GABAB, and 5-HT2A signalling directly influenced the cyclic AMP response element binding protein (CREB) expression, neuronal survival and ROS mediated cell damage which was confirmed from the gene expression of NF-kappaB, TNF-alpha, Akt-1 and SOD. In addition to enhanced hepatocyte proliferation, GABA and 5-HT chitosan nanoparticle treatment protected the neurons from ROS mediated cell damage and promoted their survival in the cerebral cortex. This has application in liver based diseases and treatments with nanosized active compounds.

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Depressed synthesis of DNA in regenerating rat liver after spinal cord (C7) transection

Cited by 10 publications

References 9 publications

Increased 5-HT2C receptor binding in the brain stem and cerebral cortex during liver regeneration and hepatic neoplasia in rats

Increased 5-HT2C receptor binding in the brain stem and cerebral cortex during liver regeneration and hepatic neoplasia in rats

Decreased synthesis of DNA in regenerating rat liver after the administration of reserpine

Increased Cortical Neuronal Survival During Liver Injury: Effect of Gamma Aminobutyric Acid and 5-HT Chitosan Nanoparticles

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