Depression of bone blood flow after blunt trauma:A fracture study in the adult rabbit

Triffitt, Paul D.; Gregg, P. J.

doi:10.3109/17453679408995433

Cited by 15 publications

(9 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At least with this method, diaphyseal ostectomy therefore does not seem to have any measurable influence on the intramedullary blood circulation of areas distant from the ostectomy. This contradicts the results of Triffitt et al (1993) as well as Triffitt and Gregg (1994). Using the microsphere method in diaphyseal osteomized rabbit tibiae, they detected an initially reduced flow in the distal part of the tibia, which did not rise until after one or two weeks.…”

Section: Discussion Of the Resultscontrasting

confidence: 42%

Continual measurement of intramedullary blood perfusion with laser Doppler flowmetry in intact and ostectomized tibiae of rabbits

2008

View full text Add to dashboard Cite

SummaryBlood flow is important for the healing of bone fractures. Until now, however, there have been no publications on the daily, continual measurement of intramedullary blood perfusion using laser Doppler flowmetry (LDF) in the conscious animal. In this study, a model for the daily, continual measurement of intramedullary blood perfusion by LDF and the temperature near the cortex both in intact and ostectomized tibiae in the conscious rabbit is described. The probes for blood perfusion and temperature measurement were implanted permanently at three different localizations into the right tibia of 10 adult New Zealand White rabbits. The probes were held in place by a bilateral, single-plane external fixator. In five of these animals, a midshaft tibial ostectomy was created in order to simulate a fracture. Intramedullary blood perfusion and temperature were measured daily over 49 days.While in intact tibiae no significant (P . 0.05) differences were found in blood perfusion readings taken at various time points, for mean values or for blood perfusion over time, in ostectomized tibiae the differences were significant: various time points (P ¼ 0.0056), mean values (P ¼ 0.0034) and blood perfusion over time (P ¼ 0.0337). Blood perfusion readings at the centre probe were elevated compared with those at the proximal and distal probes.Thus, a revascularization in the ostectomy gap during the fracture healing was proven by means of the LDF. No influence of the blood perfusion on the temperature in the ostectomy area could be determined during healing of the ostectomy. The described model seems suitable for the continual measurement of intramedullary blood perfusion both in intact and ostectomized tibiae in the conscious rabbit.

show abstract

Section: Discussion Of the Resultscontrasting

confidence: 42%

Continual measurement of intramedullary blood perfusion with laser Doppler flowmetry in intact and ostectomized tibiae of rabbits

2008

View full text Add to dashboard Cite

show abstract

“…Bone pain at sites of haemopoiesis is a consistent side-e¡ect following cytokine administration in a signi¢cant proportion of patients (Abels & Rudnick 1991, Metcalf & Nicola 1995, Gudi et al 1995. This might be the result of enhanced marrow blood £ow causing distension of intramedullary vessels and increased transport of £uid to the interstitial space, increasing its hydrostatic pressure (Iversen et al1992b(Iversen et al , 1993a.There is also some evidence that marrow blood £ow is reduced following fracture of the encasing bone (Tri¤tt & Gregg 1994), but any subsequent e¡ect on marrow function is unknown.…”

Section: Clinical Implications and Future Directionsmentioning

confidence: 99%

Blood flow to the haemopoietic bone marrow

Iversen

1997

Acta Physiologica Scandinavica

View full text Add to dashboard Cite

In order to maintain an adequate haemopoiesis, the bone marrow is dependent on a sufficient blood supply. Our understanding of the regulation of marrow perfusion remained patchy for several decades, mainly because of technological difficulties. Recent advances in methodology have permitted more detailed analyses of bone marrow perfusion. Enhanced stimulation of erythropoietic or leucopoietic activity leads to increased blood flow to the bone marrow. There is evidence that this vasodilatory effect is mediated by the cytokines erythropoietin and granulocyte colony-stimulating factor; however, a direct link has yet to be firmly established. The presence of receptors for several other cytokines on vascular endothelial and smooth muscle cells suggests that these may also have a regulatory role. The vasodilating factor nitric oxide (NO) regulates bone marrow blood flow both under normal conditions and during accelerated haemopoiesis, and NO is possibly induced via activation of cytokine receptors. There are conflicting reports as to how catecholamines affect marrow vascular tone. Although there is firm evidence that the bone marrow vasculature is innervated, no definite role of this innervation in vasoregulation has been documented. Advances in bone marrow/blood stem cell transplantation technology and the development of other therapeutic strategies for bone marrow failure may in part be dependent on optimization of the blood supply to the haemopoietic bone marrow.

show abstract

“…Briiiker and Bailey [4] showed in a clinical study that vascular injury on a macroscopic scale increases the incidence of delayed union 01-non-union. Delayed union of long bone fractures is coni~nonly ascribed to deficient vascularity [24]. Arany et al [2] reported that arterioles were only present in a sniall number in atrophic non-unions.…”

Section: Introductionmentioning

confidence: 99%

Human atrophic fracture non‐unions are not avascular

Reed

Joyner

Brownlow

et al. 2002

Journal Orthopaedic Research

132

View full text Add to dashboard Cite

A sinall proportion of fractures progress to non-union. Non-uiiioiis are routinely classified into two groups either hypertrophic or atrophic according to their radiological appearance. It is a coiiimon preconception that non-unions with a hypertrophic appearance on X-ray are biologically active and vascular with potential to heal given the correct stable environment. Atrophic nonunions are considered to be avascular aiid inert and will not heal even under the correct stable environment. Non-unions are either infected or aseptic. I n the present study, we tested the liypothesis that aseptic atrophic tion-uiiioiis are less vascular than aseptic hypertrophic lion-unions aiid healing fractures. Biopsies were taken from the fracture gap of patients with healing fractures. hypertrophic lion-unions aiid atrophic non-unions. A dual labelling technique was used with antibodies against CD3 1 (JC70) and Collagen IV. Blood vessels were quantified using a Chalkley point eyepiece graticule. There was no statistically significant difference in the median vessel count between the three fracture groups. These findings do not support tlie hypothesis that established atrophic fracture non-union are less vascular than hypertrophic non-unions or healing fractures.

show abstract

Depression of bone blood flow after blunt trauma:A fracture study in the adult rabbit

Cited by 15 publications

References 11 publications

Continual measurement of intramedullary blood perfusion with laser Doppler flowmetry in intact and ostectomized tibiae of rabbits

Continual measurement of intramedullary blood perfusion with laser Doppler flowmetry in intact and ostectomized tibiae of rabbits

Blood flow to the haemopoietic bone marrow

Human atrophic fracture non‐unions are not avascular

Contact Info

Product

Resources

About