Depth enhancement of 3D microscopic living‐cell image using incoherent fluorescent digital holography

Bang, Le Thanh; Wu, Huiying; Zhao, Yu; Kim, E.G.; Kim, N.

doi:10.1111/jmi.12510

Cited by 4 publications

(1 citation statement)

References 20 publications

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“…Enhanced phase contrast of DHM would likely reveal more subtle morphological distinctions between cells cultured on different substrates and at different phases. This could be achieved in the future through improvements to reconstruction algorithms and DHM optical systems , including using super‐resolution set‐ups .…”

Section: Discussionmentioning

confidence: 99%

Quantitative assessment of cancer cell morphology and motility using telecentric digital holographic microscopy and machine learning

et al. 2017

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The noninvasive, fast acquisition of quantitative phase maps using digital holographic microscopy (DHM) allows tracking of rapid cellular motility on transparent substrates. On two-dimensional surfaces in vitro, MDA-MB-231 cancer cells assume several morphologies related to the mode of migration and substrate stiffness, relevant to mechanisms of cancer invasiveness in vivo. The quantitative phase information from DHM may accurately classify adhesive cancer cell subpopulations with clinical relevance. To test this, cells from the invasive breast cancer MDA-MB-231 cell line were cultured on glass, tissue-culture treated polystyrene, and collagen hydrogels, and imaged with DHM followed by epifluorescence microscopy after staining F-actin and nuclei. Trends in cell phase parameters were tracked on the different substrates, during cell division, and during matrix adhesion, relating them to F-actin features. Support vector machine learning algorithms were trained and tested using parameters from holographic phase reconstructions and cell geometric features from conventional phase images, and used to distinguish between elongated and rounded cell morphologies. DHM was able to distinguish between elongated and rounded morphologies of MDA-MB-231 cells with 94% accuracy, compared to 83% accuracy using cell geometric features from conventional brightfield microscopy. This finding indicates the potential of DHM to detect and monitor cancer cell morphologies relevant to cell cycle phase status, substrate adhesion, and motility. © 2017 International Society for Advancement of Cytometry.

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Section: Discussionmentioning

confidence: 99%

Quantitative assessment of cancer cell morphology and motility using telecentric digital holographic microscopy and machine learning

et al. 2017

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All-dielectric bifocal isotropic metalens for a single-shot hologram generation device

Zhou

Huang

et al. 2020

Opt. Express

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Planar metalenses are regarded as promising functional nanodevices because of their lightweight, nano-resolution properties, and, therefore, they can serve as versatile platforms for imaging and Fourier transforming. Here, we demonstrate a meta-device that functions as an isotropic bifocal all-dielectric Huygens' metalens to realize nanoscale real-time coaxial digital hologram generation. We design an isotropic bifocal metalens for micro/nano hologram recording, and the metalens utilizes the complete region compared to a previously reported interleaved multifocal metalens scheme. In addition, the hologram generation does not depend on complex polarization conversion, thereby improving the practical efficiency. For high-fidelity reconstruction, compressive reconstruction is utilized to remove twin-image and zero-order items and to suppress noise. Such concept would be extended to white-light achromatic meta-holography and three-dimensional micro/nano in vivo incoherent super-resolution imaging under subwavelength modulation.

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3D fluorescence imaging through scattering medium using transport of intensity equation and iterative phase retrieval

Matsuda,

Shoda,

Yoneda

et al. 2024

Opt. Express

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In this paper, we have proposed a method of three-dimensional (3D) fluorescence imaging through a scattering medium. The proposed method combines the numerical digital phase conjugation propagation after measurement of the complex amplitude distribution of scattered light waves by the transport of intensity equation (TIE) with followed iterative phase retrieval to achieve 3D fluorescence imaging through a scattering medium. In the experiment, we present the quantitative evaluation of the depth position of fluorescent beads. In addition, for time-lapse measurement, cell division of tobacco-cultured cells was observed. Numerical results presented the effective range of the phase amount in the scattering medium. From these results, the proposed method is capable of recovering images degraded by a thin scattering phase object beyond a small phase change approximation.

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Depth enhancement of 3D microscopic living‐cell image using incoherent fluorescent digital holography

Cited by 4 publications

References 20 publications

Quantitative assessment of cancer cell morphology and motility using telecentric digital holographic microscopy and machine learning

Quantitative assessment of cancer cell morphology and motility using telecentric digital holographic microscopy and machine learning

All-dielectric bifocal isotropic metalens for a single-shot hologram generation device

3D fluorescence imaging through scattering medium using transport of intensity equation and iterative phase retrieval

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