Depth-resolved chromophore analysis of bovine retina and pigment epithelium by photoacoustic spectroscopy

Boucher, F.; Leblanc, Roger M.; Savage, Sylvie; Beaulieu, Bruno

doi:10.1364/ao.25.000515

Cited by 12 publications

(3 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Zeaxanthin was obtained from Hoffmann-La Roche Co. formed with a Perkin-Elmer 553 spectrophotometer. For the photoacoustic determinations, a home-made photoacoustic spectrometer (39)(40)(41)(42)(43) provided with Challenger I1 or Apple 11 computer was utilized.…”

Section: Methodsmentioning

confidence: 99%

Absorption and photoacoustic spectroscopies of lutein and zeaxanthin Langmuir–Blodgett films in connection with the Haidinger's brushes

N’soukpoé‐Kossi¹,

Leblanc²

1988

Can. J. Chem.

View full text Add to dashboard Cite

The molecular state of a single monolayer of lutein, zeaxanthin, and of a mixture of lutein and L-a-phosphatidylcholine-P-oleoyl-y-stearoyl has been investigated using absorption and photoacoustic spectroscopies. Both methods, confirmatively, reveal that the two carotenoids are mostly in a monomer state coexisting with carotenoid crystals. We observe that the absorption as well as the photoacoustic bands are red-shifted relative to the bands in solution. The absorption bands are found to peak at mean values of 440.0, 464.8, and 503.8 -t 0.5 nm for lutein, and at 452.7, 475.9, and 513.7 -t 0.5 nm for zeaxanthin. The photoacoustic peaks are situated at 444.0, 467.2, and 502.4 5 0.5 nm for lutein, and 455.4, 473.8, and 509.0 2 0.5 nm for zeaxanthin. Diluting lutein monolayer with the phospholipid results into a blue-shift of the absorption bands compared with those of the pure carotenoid monolayer and the macular pigment spectra. The results are discussed in connection with the Haidinger's brushis. It is concluded that lutein molecules might not be mixed with membrane lipids in the macular tissue, but form a monolayer protruding from the Henle fiber membranes to form the Haidinger's brushes in the macula. zeaxanthine. Lors de la dilution de la monocouche de luttine avec le phospholipide, les bandes observtes sont dtplactes vers le bleu par rapport a celles obtenues dans le spectre de la monocouche de carottno'ide pur ainsi dans celui du pigment maculeux. On discute des rtsultats en fonction des brosses de Haidinger. On en conclut que les moltcules de luttine ne sont peut Ctre pas milangees avec les lipides membranaires dans le tissu maculeux; toutefois, elles forment une rnonocouche faisant saillie par rapport aux fibres membranaires de Henle et elles conduisent a la formation des brosses de Haidinger dans la macule.[Traduit par la revue]

show abstract

Section: Methodsmentioning

confidence: 99%

Absorption and photoacoustic spectroscopies of lutein and zeaxanthin Langmuir–Blodgett films in connection with the Haidinger's brushes

N’soukpoé‐Kossi¹,

Leblanc²

1988

Can. J. Chem.

View full text Add to dashboard Cite

show abstract

“…Photoacoustic (PA) measurements were performed on a homemade PA spectrometer (Ducharme et al, 1979; Boucher and Leblanc, 1985; Boucher et al, 1986) consisting of a 1000-W xenon lamp (Schoeffel Instrument Corp., Westwood, NJ) and a monochromator driven by a variable-speed motor. The monochromatic beam was modulated by a mechanical chopper at a frequency that could be varied from 20 to 1000 Hz, and it was focussed and reflected into the PA cell.…”

Section: Methodsmentioning

confidence: 99%

Kinetic study of Maillard reactions in milk powder by photoacoustic spectroscopy

N’soukpoé‐Kossi¹,

Martel²,

Leblanc³

et al. 1988

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

“…Furthermore, the conven-tionaI technique can not be used for the direct measurement of thermal decay of excited molecules, being related to the investigation of the energy storage. These technical limitations can be overcome by using the photoacoustic spectroscopic technique as previously proved successful for the studies on the absorption properties of the cattle retina (Boucher and Leblanc, 1981a;Boucher et al, 1986) and the confirmation of energy storage in the primary photoreaction of rhodopsin (Boucher and Leblanc, 1985).…”

Section: Introductionmentioning

confidence: 99%

LOW‐TEMPERATURE PHOTOACOUSTIC SPECTROSCOPY OF PORPHYROPSIN IN THE INTACT RETINA OF THE CREEK CHUB, Semotilus atromaculatus

Yoon

Leblanc

Ali

et al. 1988

Photochem & Photobiology

Self Cite

View full text Add to dashboard Cite

Abstract— The photoacoustic spectra of porphyropsin in the creek chub (Semotilus atromaculatus) have been successfully measured on single retinae of small size (< 0.5 cm in diameter) at low temperatures. The relative intensities of the spectra, corresponding to the thermal release, are strong enough to be comparable to those for the big cattle retina. The photoacoustic spectrum of porphyropsin at – 167°C exhibits a principal maximum at 540 nm with a small shoulder near 580 nm. A sequence of porphyropsin bleaching resulted from thermal reaction has been clearly observed above ‐90°C.

show abstract

Depth-resolved chromophore analysis of bovine retina and pigment epithelium by photoacoustic spectroscopy

Cited by 12 publications

References 12 publications

Absorption and photoacoustic spectroscopies of lutein and zeaxanthin Langmuir–Blodgett films in connection with the Haidinger's brushes

Absorption and photoacoustic spectroscopies of lutein and zeaxanthin Langmuir–Blodgett films in connection with the Haidinger's brushes

Kinetic study of Maillard reactions in milk powder by photoacoustic spectroscopy

LOW‐TEMPERATURE PHOTOACOUSTIC SPECTROSCOPY OF PORPHYROPSIN IN THE INTACT RETINA OF THE CREEK CHUB, Semotilus atromaculatus

Contact Info

Product

Resources

About