Der Blutmonocyt

Murine leukocytes with ring-shaped nuclei include granulocytes, monocytes, and their precursors

“…In contrast, other authors have suggested that macrophages (1) may derive from more than one progenitor cell or lineage and (2) may additionally derive from cells other than the typical kidney-shaped blood monocyte, e.g., from proliferating local macrophages [72][73][74]. Leder found cells in BM and PB with strong activity of both unspecific esterase and chloroacetate esterase and concluded that they may present an intermediate form between the granulocytic (promyelocyte) and monocytic (monocyte) lineage [74,75]. Metcalf also had evidence that murine macrophages in vitro could arise by transformation of granulocyte precursors [69,70].…”

“…Although sorter analysis revealed that some of the double-positive cells were GR-1 low and not GR-1 high as granulocytes, it remains a fact that some cells with closed and especially with ring-shaped nuclei cannot be clearly assigned to the granulocytic or monocytic lineage by conventional methods. These cells could present so-called bipotential cells with common characteristics of monocytes and granulocytes beyond the stage of the GM-CFU myelomonocytic stem cell [69,70,74,75].…”

Infectious Lymphocytes in Lymphocytic Choriomeningitis Virus Carrier Mice

“…Smears of cytocentrifuge preparations (' Cytospin', Shandon Labortechnik GmbH, Frankfurt, Federal Republic of Germany) were stained with May-Grfinwald-Giemsa. Acid phosphatase and non-specific esterase were demonstrated histochemically using standard procedures (Leder, 1967).…”

Isolation and properties of cell lines from the metastasising rat mammary tumour SMT-2A

“…The Rama 950 adherent feeder cells are non-neoplastic host cells, and the presence of intermediate filaments, microvilli and basement membrane proteins in the majority and the lack of staining with anti-factor VIII serum (Kiyoshi et al, 1980) or for non-specific esterase (Leder, 1967) distinguishes them from fibroblasts, endothelial cells or macrophages. However, the epithelial-like features of Rama 950 cells are more akin to those of mesothelial cells.…”