Murine leukocytes with ring-shaped nuclei include granulocytes, monocytes, and their precursors

Biermann, Heike; Pietz, Barbara; Dreier, Rita; Schmid, Kurt Werner; Sorg, Clemens; Sunderkötter, Cord

doi:10.1002/jlb.65.2.217

Cited by 107 publications

(78 citation statements)

References 46 publications

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“…The other population (Ly-6C hi ) shows slight positivity with anti-Ly-6G mAbs with certain anti-Ly-6C and anti-Ly-6G fluorochrome combinations. This population is comprised of macrophage and dendritic cell precursors (unpublished observations), and cells of the monocytic lineage have previously been reported to express low levels of Ly-6G (10,11). Thus, this slight shift could represent detection of true, low-level Ly-6G expression on this population that is apparent with certain fluorochromemAb conjugates.…”

Section: Discussionmentioning

confidence: 84%

“…Ly-6G, another member of the Ly-6 family, is expressed on granulocytes, a subset of eosinophils, and transiently during developmental stages of monocytes (7)(8)(9)(10)(11). Its expression has been studied primarily using the monoclonal antibody (mAb) RB6-8C5 (anti Gr-1), but other monoclonal antibodies (mAbs) have also been developed against this antigen (Ag), including NIMPR-14 (9).…”

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confidence: 99%

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Absence of cross‐reactivity between murine Ly‐6C and Ly‐6G

Nagendra

Schlueter

2004

Cytometry Pt A

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BackgroundThe Ly‐6 family has many members, including Ly‐6C and Ly‐6G. A previous study suggested that the anti‐Ly‐6G antibody, RB6‐8C5, may react with Ly‐6Chi murine bone marrow (BM) cells. This finding has been interpreted as cross‐reactivity of RB6‐8C5 with the Ly‐6C antigen, and has been generalized to many hematopoietic cell types, using the terminology Ly‐6G/C. The present study was undertaken to determine whether anti‐Ly‐6G antibodies truly cross‐react with the Ly‐6C antigen on multiple hematopoietic cell types.MethodsSplenocytes, thymocytes, and BM cells obtained from Ly‐6.1 and Ly‐6.2 strains of mice were stained with a variety of antibodies to Ly‐6C and Ly‐6G. Flow cytometric analysis was performed on these populations.ResultsEvaluation of anti‐Ly‐6C and anti‐Ly‐6G staining showed only Ly‐6C expression and no Ly‐6G expression on subsets of splenic T and B cells and thymocytes from Ly‐6.1 and Ly‐6.2 mice. Bone marrow cells were identified that express both Ly‐6G and Ly‐6C; no Ly‐6G+Ly‐6C− populations were seen.ConclusionsMultiple Ly‐6C+ hematopoietic cell populations were identified that do not stain with anti‐Ly‐6G antibodies. This calls into question the use of the Ly‐6G/C nomenclature and suggests that epitopes recognized by anti‐Ly‐6G antibodies should simply be designated Ly‐6G. © 2004 Wiley‐Liss, Inc.

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Section: Discussionmentioning

confidence: 84%

mentioning

confidence: 99%

Absence of cross‐reactivity between murine Ly‐6C and Ly‐6G

Nagendra

Schlueter

2004

Cytometry Pt A

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“…Similar cells are found in the bone marrow and have a cytoplasmic center that is smaller than the width of the ring (8). The presence of these cells suggests that immature myeloid precursor cells originating in the bone marrow may have been recruited to the peritoneal cavity (8).…”

Section: Morphologic Characterization Of Cells Following Mbsa-inducedmentioning

confidence: 74%

The Phenotype of Inflammatory Macrophages Is Stimulus Dependent: Implications for the Nature of the Inflammatory Response

Cook

Braine

Hamilton

2003

The Journal of Immunology

129

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Many diseases are characterized by inflammatory reactions involving both the innate and adaptive arms of the immune system. Thioglycolate medium (TM) injection into the peritoneal cavity has long been used as a stimulus for eliciting inflammatory macrophages for study and for determining the importance of a particular mediator in inflammation. However, the response to this irritant may not be relevant to many inflammatory diseases. Therefore, we have developed an Ag-specific peritonitis model using methylated BSA (mBSA) as the stimulus. Priming mice intradermally with mBSA in adjuvant and boosting 14 days later, followed by an i.p. challenge with mBSA after an additional 7 days, led to an inflammatory reaction equivalent in magnitude to that induced with TM as judged by the number of exudate cells. The inflammatory macrophages elicited by the mBSA protocol differed, being smaller and less vacuolated than TM-elicited macrophages. Also, macrophages from 4-day mBSA-induced exudates expressed more MHC class II than TM-induced exudates, were able to stimulate allogeneic T lymphocytes, and upon in vitro stimulation with LPS secreted greater levels of IL-6 and IL-1β. Macrophages from 4-day TM-induced exudates, on the other hand, expressed Ly6C and ER-MP58, immature myeloid markers. The inflammatory response elicited using the Ag mBSA may be more relevant for studying the inflammatory responses in many diseases, such as those of autoimmune origin and those involving an acquired immune response.

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“…Why is the mouse granulocyte nucleus ring-shaped; [53] whereas the LBR-deficient granulocytic form is ovoid? We have speculated [32] that the ring-shape may be related to the frequently described "rosette" arrangement of chromosomes at the metaphase plate.…”

Section: Discussionmentioning

confidence: 99%

Granulocytic nuclear differentiation of lamin B receptor–deficient mouse EPRO cells

Zwerger

Herrmann

Gaines

et al. 2008

Experimental Hematology

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Objective-Lamin B receptor (LBR) is an integral protein of the inner nuclear membrane. Recent studies have demonstrated that genetic deficiency of LBR during granulopoiesis results in hypolobulation of the mature neutrophil nucleus, as observed in human Pelger-Huët anomaly (PHA) and mouse ichthyosis (ic). In this study we have utilized differentiated promyelocytes (EPRO cells) that were derived from the bone marrow of homozygous and heterozygous ichthyosis mice to examine changes to the expression of nuclear envelope proteins and heterochromatin structure that result from deficient LBR expression.Materials and Methods-Wildtype (+/+), heterozygous (+/ic) and homozygous (ic/ic) granulocytic forms of EPRO cells were analyzed for the expression of multiple lamins and inner nuclear envelope proteins by immunostaining and immunoblotting techniques. The heterochromatin architecture was also examined by immunostaining for histone lysine methylation.Results-Wildtype (+/+) and heterozygous (+/ic) granulocytic forms revealed ring-shaped nuclei and contained LBR within the nuclear envelope; ic/ic granulocytes exhibited smaller ovoid nuclei devoid of LBR. The pericentric heterochromatin of undifferentiated and granulocytic ic/ic cells was condensed into larger spots and shifted away from the nuclear envelope, compared to +/+ and +/ic cell forms. Lamin A/C, which is normally not present in mature granulocytes, was significantly elevated in LBR-deficient EPRO cells.Conclusions-Our observations suggest roles for LBR during granulopoiesis which may involve augmenting nuclear membrane growth, facilitating compartmentalization of heterochromatin and promoting down-regulation of lamin A/C expression. Category Granulopoiesis

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Murine leukocytes with ring-shaped nuclei include granulocytes, monocytes, and their precursors

Cited by 107 publications

References 46 publications

Absence of cross‐reactivity between murine Ly‐6C and Ly‐6G

Absence of cross‐reactivity between murine Ly‐6C and Ly‐6G

The Phenotype of Inflammatory Macrophages Is Stimulus Dependent: Implications for the Nature of the Inflammatory Response

Granulocytic nuclear differentiation of lamin B receptor–deficient mouse EPRO cells

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