Der Einflu� von Glukagon und Insulin auf den Stoffwechsel der nicht veresterten Fetts�uren am isolierten Fettgewebe der Ratte in vitro

Weinges, K. F.

doi:10.1007/bf01482738

Cited by 101 publications

(5 citation statements)

References 23 publications

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“…Under these conditions, we can observe a suppression of the glucagon-stimulated effect, i. e. a drop in plasma FFA. Our in vivo results are similar to those obtained in vitro on rat adipose tissue by Weinges [28] who showed that FFA release can be inhibited both by high concentrations of glucose in the absence of added insulin, or by addition of insulin at normal glucose concentrations.…”

Section: The Effect Of Pancreatectomy On Plasma Ffasupporting

confidence: 90%

Roles of insulin and glucose in the regulation of plasma FFA in the duck

Gross¹,

Mialhe²

1978

Diabetologia

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The present study was undertaken to determine how free fatty acid (FFA) release by adipose tissue can occur in the absence of pancreatic glucagon, the most potent lipolytic hormone in the duck. Two possible explanations have successively been investigated. Replacement therapy experiments showed that glucose uptake was implicated in the phenomenon; indeed, when pre-operative insulin and glucose levels were restored after total pancreatectomy, plasma FFA progressively fell to a level significantly lower than the pre-operative value. The same effect was observed when the ducks were kept in a continuous state of hyperglycaemia, whereas "normal" levels of insulin or glucose alone were shown to be ineffective. On the other hand a compensatory mechanism for the lack of glucagon, by other lipolytic agents [with the intestine or the anterior pituitary as possible sources] has been excluded by associating the removal of these two organs with pancreatectomy. These experiments suggest that the lack of pancreatic glucagon induced by total pancreatectomy is essentially masked by a failure in glucose uptake due to the operation.

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Section: The Effect Of Pancreatectomy On Plasma Ffasupporting

confidence: 90%

Roles of insulin and glucose in the regulation of plasma FFA in the duck

Gross¹,

Mialhe²

1978

Diabetologia

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“…In vitro, glucagon increases free fatty acid release from rat adipose tissue (STEINBEI~G et al, 1959; HA-GEN, 1960;WEINGES, 1961). This effect results from increased lipolysis (WEINOES and L6F]~L]~R, 1965) with concomitant increased re~sterification as shown by VAVGHAN and STEI~BEaG (1963) with their "non isotopic balance method", and by ourselves (LEFEBV~E, 1966).…”

Section: Effet Physiologique Du Glucagon Sur La Mobilisation Des Lipimentioning

confidence: 93%

“…Die intraportale Infusion von physiologisehen Dosen von Glucagon, das mit Cystein behandelt ist (0.002 #g/kg/min) erhSht bei normalen Hunden unter Pentobarbital-Anaesthesie eindeutig die Plasmaspiegel der freien Fetts~uren im peripheren Blur. Dieset Effekt kann als Hinweis auf die physiologische Bedeutung des Glucagons im Fettstoffwechsel angesehen werden.In vitro, glucagon increases free fatty acid release from rat adipose tissue (STEINBEI~G et al, 1959; HA-GEN, 1960;WEINGES, 1961). This effect results from increased lipolysis (WEINOES and L6F]~L]~R, 1965) with concomitant increased re~sterification as shown by VAVGHAN and STEI~BEaG (1963) with their "non isotopic balance method", and by ourselves (LEFEBV~E, 1966).…”

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The physiological effect of glucagon on fat-mobilisation

Lefèbvre

1966

Diabetologia

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Summary. In normal dogs under pentobarbital anaesthesia, intraportal infusion of physiological doses (0.002 /~g/kg/min) of cystein-treated glucagon increases markedly the plasma FFA levels in the peripheral blood. This can be considered as an argument for a possible role of glucagon as a physiological regulator of lipid metabolism. Effet physiologique du glucagon sur la mobilisation des lipides.Rgsumd. La perfusion portMe de doses physiologiques (0.002 #g/kg/min) de glucagon trait6 par la cystdine augmente de fagon trbs signifieative le taux sanguin des aeides gras libres du plasma pgriphdrique. Cette observation constitue un argument en faveur d'un r61e physiologique du glucagon dans le m6tabolisme des lipides.Der physiologischc Effe/ct yon Glucagon auf die ~ett-mobilisation.Zusammenfassung. Die intraportale Infusion von physiologisehen Dosen von Glucagon, das mit Cystein behandelt ist (0.002 #g/kg/min) erhSht bei normalen Hunden unter Pentobarbital-Anaesthesie eindeutig die Plasmaspiegel der freien Fetts~uren im peripheren Blur. Dieset Effekt kann als Hinweis auf die physiologische Bedeutung des Glucagons im Fettstoffwechsel angesehen werden.In vitro, glucagon increases free fatty acid release from rat adipose tissue (STEINBEI~G et al., 1959; HA-GEN, 1960;WEINGES, 1961). This effect results from increased lipolysis (WEINOES and L6F]~L]~R, 1965) with concomitant increased re~sterification as shown by VAVGHAN and STEI~BEaG (1963) with their "non isotopic balance method", and by ourselves (LEFEBV~E, 1966).An effect on FFA release has been detected by WEING]SS (1961) with concentrations of glucagon of 0.004 #g/ml and by LV.FEBV~E (1966) with concentrations of 0.002/~g/ml, which are in the range of the physiological levels evaluated in plasma by U~GER et al. (1962).In vivo, the effects of glucagon on plasma FFA have led to conflicting results: a fall in plasma FFA in man was observed by LrSSETT et al. (1960), PENNICK and tIINKLE (1960), EY~ER et al. (1961 and D~EmI~G et al. (1962), andin dog by STEr~BERG et al. (1959); an early rise by WEIZCGES (1961) in man, and by LEVARLET (1962) in dog; an initial fall and a secondary rise, in man, byFELBER and van ITALLIE (1958) and by LIPSETT et al. (1960). We recently reported (LE]~V~BV~E, 1965) that, in man, glucagon at the dose of 1 mg/m ~, induces an initial fall of plasma FFA followed by a marked secondary rise, which can be observed between the 2nd and 5th hour after the injection. This initial fall can be reproduced by an hyperglycemia of the same magnitude as the one caused by glueagon, and induced by i. v. glucose infusion. The secondary rise is much more important after glucagon than the small rebound-effect observed after glucose. This secondary rise could correspond to the fat-mobilizing effect of the hormone.Nevertheless the physiological signification of these experiments has not been established. In the experiments reported here, we studied the effects on plasma FFA of physiological doses of glucagon perfused in the physiological site of secretion of the ho...

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“…Such differences observed in the effects of glucagon have yielded conflicting interpretations about the mechanisms involved in in vivo effect of glucagon. Moreover, there has been no report of glucagon effect on blood FFA in rats, although a lipolytic action of glucagon has been shown mainly in rat adipose tissue in vitro (HAGEN, 1961;STEINBERG et al, 1959;VAUGHAN and STEINBERG, 1963;WEINIGES, 1961;WEINIGES and LOEFFLER, 1965). The explanation for this fact might be in part found in a difficulty in succesive sampling of blood under non-stress conditions from such a small animal as rat.…”

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confidence: 99%